Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1451 and Shew_1452 are separated by 242 nucleotides Shew_1452 and Shew_1453 are separated by 65 nucleotides Shew_1453 and Shew_1454 are separated by 114 nucleotides
Shew_1451: Shew_1451 - ABC transporter-related protein (RefSeq), at 1,671,372 to 1,673,201
_1451
Shew_1452: Shew_1452 - AraC family transcriptional regulator (RefSeq), at 1,673,444 to 1,674,955
_1452
Shew_1453: Shew_1453 - methylated-DNA--protein-cysteinemethyltransferase (RefSeq), at 1,675,021 to 1,675,608
_1453
Shew_1454: Shew_1454 - DEAD/DEAH box helicase domain-containing protein (RefSeq), at 1,675,723 to 1,677,048
_1454
Position (kb)
1673
1674
1675 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1672.485 kb on + strand, within Shew_1451 at 1672.644 kb on - strand, within Shew_1451 at 1672.746 kb on - strand, within Shew_1451 at 1672.758 kb on + strand, within Shew_1451 at 1673.050 kb on - strand at 1673.472 kb on - strand at 1673.663 kb on + strand, within Shew_1452 at 1673.778 kb on - strand, within Shew_1452 at 1673.922 kb on + strand, within Shew_1452 at 1674.090 kb on + strand, within Shew_1452 at 1674.339 kb on + strand, within Shew_1452 at 1674.366 kb on - strand, within Shew_1452 at 1674.366 kb on - strand, within Shew_1452 at 1674.795 kb on - strand, within Shew_1452 at 1674.945 kb on - strand at 1675.256 kb on - strand, within Shew_1453 at 1675.321 kb on - strand, within Shew_1453 at 1675.414 kb on - strand, within Shew_1453 at 1675.761 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM remove 1,672,485 + Shew_1451 0.61 -0.1 1,672,644 - Shew_1451 0.70 +0.1 1,672,746 - Shew_1451 0.75 +0.8 1,672,758 + Shew_1451 0.76 +0.3 1,673,050 - -0.3 1,673,472 - +0.0 1,673,663 + Shew_1452 0.14 +0.3 1,673,778 - Shew_1452 0.22 -0.1 1,673,922 + Shew_1452 0.32 -0.7 1,674,090 + Shew_1452 0.43 -0.1 1,674,339 + Shew_1452 0.59 -0.0 1,674,366 - Shew_1452 0.61 -0.3 1,674,366 - Shew_1452 0.61 -0.4 1,674,795 - Shew_1452 0.89 -2.9 1,674,945 - +0.1 1,675,256 - Shew_1453 0.40 -0.7 1,675,321 - Shew_1453 0.51 -0.3 1,675,414 - Shew_1453 0.67 +1.0 1,675,761 - +1.3
Or see this region's nucleotide sequence