Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1423 and Shew_1424 are separated by 159 nucleotides Shew_1424 and Shew_1425 are separated by 62 nucleotides Shew_1425 and Shew_1426 are separated by 177 nucleotides
Shew_1423: Shew_1423 - sulfate adenylyltransferase, large subunit (RefSeq), at 1,637,085 to 1,638,542
_1423
Shew_1424: Shew_1424 - TrkA domain-containing protein (RefSeq), at 1,638,702 to 1,640,429
_1424
Shew_1425: Shew_1425 - adenylyl-sulfate kinase (RefSeq), at 1,640,492 to 1,641,121
_1425
Shew_1426: Shew_1426 - hypothetical protein (RefSeq), at 1,641,299 to 1,642,765
_1426
Position (kb)
1638
1639
1640
1641 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1637.845 kb on - strand, within Shew_1423 at 1638.222 kb on - strand, within Shew_1423 at 1638.857 kb on + strand at 1638.885 kb on - strand, within Shew_1424 at 1639.123 kb on + strand, within Shew_1424 at 1639.158 kb on - strand, within Shew_1424 at 1639.299 kb on - strand, within Shew_1424 at 1639.420 kb on - strand, within Shew_1424 at 1639.727 kb on - strand, within Shew_1424 at 1640.035 kb on + strand, within Shew_1424 at 1640.035 kb on + strand, within Shew_1424 at 1640.318 kb on + strand at 1641.027 kb on + strand, within Shew_1425 at 1641.042 kb on - strand, within Shew_1425
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM remove 1,637,845 - Shew_1423 0.52 -2.3 1,638,222 - Shew_1423 0.78 -1.7 1,638,857 + -1.8 1,638,885 - Shew_1424 0.11 -2.8 1,639,123 + Shew_1424 0.24 -2.2 1,639,158 - Shew_1424 0.26 -5.5 1,639,299 - Shew_1424 0.35 -1.1 1,639,420 - Shew_1424 0.42 -5.2 1,639,727 - Shew_1424 0.59 -3.3 1,640,035 + Shew_1424 0.77 -3.8 1,640,035 + Shew_1424 0.77 -1.9 1,640,318 + -1.8 1,641,027 + Shew_1425 0.85 +3.4 1,641,042 - Shew_1425 0.87 +3.3
Or see this region's nucleotide sequence