Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1226 and Shew_1227 are separated by 198 nucleotides Shew_1227 and Shew_1228 are separated by 2 nucleotides Shew_1228 and Shew_1229 are separated by 98 nucleotides
Shew_1226: Shew_1226 - peptidase M16 domain-containing protein (RefSeq), at 1,423,434 to 1,426,268
_1226
Shew_1227: Shew_1227 - glutamate--cysteine ligase (RefSeq), at 1,426,467 to 1,428,038
_1227
Shew_1228: Shew_1228 - hypothetical protein (RefSeq), at 1,428,041 to 1,428,481
_1228
Shew_1229: Shew_1229 - hypothetical protein (RefSeq), at 1,428,580 to 1,429,197
_1229
Position (kb)
1426
1427
1428
1429 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1425.544 kb on + strand, within Shew_1226 at 1425.562 kb on - strand, within Shew_1226 at 1425.677 kb on + strand, within Shew_1226 at 1425.766 kb on + strand, within Shew_1226 at 1425.824 kb on + strand, within Shew_1226 at 1425.886 kb on - strand, within Shew_1226 at 1425.944 kb on + strand, within Shew_1226 at 1426.008 kb on - strand at 1426.153 kb on - strand at 1426.241 kb on - strand at 1426.602 kb on + strand at 1426.706 kb on + strand, within Shew_1227 at 1427.665 kb on - strand, within Shew_1227 at 1428.879 kb on + strand, within Shew_1229
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM remove 1,425,544 + Shew_1226 0.74 +0.9 1,425,562 - Shew_1226 0.75 +0.4 1,425,677 + Shew_1226 0.79 -2.0 1,425,766 + Shew_1226 0.82 +0.5 1,425,824 + Shew_1226 0.84 +0.0 1,425,886 - Shew_1226 0.86 +0.0 1,425,944 + Shew_1226 0.89 +0.8 1,426,008 - +0.1 1,426,153 - -0.4 1,426,241 - -1.0 1,426,602 + -4.3 1,426,706 + Shew_1227 0.15 -3.3 1,427,665 - Shew_1227 0.76 -3.3 1,428,879 + Shew_1229 0.48 -2.5
Or see this region's nucleotide sequence