Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0085 and Shew_0086 are separated by 260 nucleotides Shew_0086 and moaA are separated by 315 nucleotides
Shew_0085: Shew_0085 - methyl-accepting chemotaxis sensory transducer (RefSeq), at 97,414 to 99,330
_0085
Shew_0086: Shew_0086 - electron-transferring-flavoproteindehydrogenase (RefSeq), at 99,591 to 101,240
_0086
Shew_0087: moaA - molybdenum cofactor biosynthesis protein A (RefSeq), at 101,556 to 102,569
moaA
Position (kb) 99
100
101
102 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 98.628 kb on + strand, within Shew_0085 at 98.834 kb on - strand, within Shew_0085 at 99.029 kb on + strand, within Shew_0085 at 99.037 kb on - strand, within Shew_0085 at 99.117 kb on + strand, within Shew_0085 at 99.135 kb on + strand, within Shew_0085 at 99.179 kb on + strand at 99.179 kb on - strand at 99.179 kb on + strand at 99.210 kb on + strand at 99.293 kb on - strand at 99.352 kb on - strand at 99.352 kb on - strand at 99.352 kb on - strand at 99.363 kb on + strand at 99.365 kb on + strand at 99.417 kb on + strand at 99.640 kb on + strand at 99.757 kb on + strand, within Shew_0086 at 99.813 kb on - strand, within Shew_0086 at 99.814 kb on - strand, within Shew_0086 at 99.830 kb on + strand, within Shew_0086 at 100.019 kb on - strand, within Shew_0086 at 100.115 kb on + strand, within Shew_0086 at 100.145 kb on - strand, within Shew_0086 at 100.209 kb on + strand, within Shew_0086 at 100.228 kb on + strand, within Shew_0086 at 100.245 kb on + strand, within Shew_0086 at 100.253 kb on - strand, within Shew_0086 at 100.294 kb on + strand, within Shew_0086 at 100.326 kb on - strand, within Shew_0086 at 100.326 kb on - strand, within Shew_0086 at 100.427 kb on - strand, within Shew_0086 at 100.546 kb on + strand, within Shew_0086 at 100.546 kb on + strand, within Shew_0086 at 100.729 kb on - strand, within Shew_0086 at 100.740 kb on - strand, within Shew_0086 at 100.846 kb on + strand, within Shew_0086 at 100.972 kb on - strand, within Shew_0086 at 101.020 kb on - strand, within Shew_0086 at 101.178 kb on + strand at 101.564 kb on + strand at 101.755 kb on + strand, within moaA at 101.864 kb on - strand, within moaA at 101.956 kb on + strand, within moaA at 101.956 kb on + strand, within moaA at 101.956 kb on + strand, within moaA at 101.964 kb on - strand, within moaA at 101.964 kb on - strand, within moaA at 102.046 kb on - strand, within moaA at 102.118 kb on + strand, within moaA at 102.126 kb on - strand, within moaA
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM remove 98,628 + Shew_0085 0.63 -0.8 98,834 - Shew_0085 0.74 -0.1 99,029 + Shew_0085 0.84 -0.3 99,037 - Shew_0085 0.85 -0.2 99,117 + Shew_0085 0.89 -0.9 99,135 + Shew_0085 0.90 -0.1 99,179 + +0.7 99,179 - +0.4 99,179 + -1.3 99,210 + +0.6 99,293 - -0.2 99,352 - -0.3 99,352 - +0.1 99,352 - +0.3 99,363 + -0.1 99,365 + +0.5 99,417 + -0.4 99,640 + +3.3 99,757 + Shew_0086 0.10 +3.3 99,813 - Shew_0086 0.13 +2.2 99,814 - Shew_0086 0.14 +3.0 99,830 + Shew_0086 0.14 +2.7 100,019 - Shew_0086 0.26 +3.2 100,115 + Shew_0086 0.32 +3.0 100,145 - Shew_0086 0.34 +3.0 100,209 + Shew_0086 0.37 +2.8 100,228 + Shew_0086 0.39 +2.8 100,245 + Shew_0086 0.40 +3.0 100,253 - Shew_0086 0.40 +3.5 100,294 + Shew_0086 0.43 +3.0 100,326 - Shew_0086 0.45 +3.1 100,326 - Shew_0086 0.45 +3.0 100,427 - Shew_0086 0.51 +3.3 100,546 + Shew_0086 0.58 +3.1 100,546 + Shew_0086 0.58 +2.8 100,729 - Shew_0086 0.69 +1.8 100,740 - Shew_0086 0.70 +3.7 100,846 + Shew_0086 0.76 +3.3 100,972 - Shew_0086 0.84 +3.0 101,020 - Shew_0086 0.87 +3.2 101,178 + +3.5 101,564 + +0.5 101,755 + moaA Shew_0087 0.20 +0.9 101,864 - moaA Shew_0087 0.30 +0.4 101,956 + moaA Shew_0087 0.39 +0.9 101,956 + moaA Shew_0087 0.39 -1.8 101,956 + moaA Shew_0087 0.39 -0.3 101,964 - moaA Shew_0087 0.40 -0.1 101,964 - moaA Shew_0087 0.40 +1.2 102,046 - moaA Shew_0087 0.48 -0.4 102,118 + moaA Shew_0087 0.55 -3.3 102,126 - moaA Shew_0087 0.56 +0.8
Or see this region's nucleotide sequence