Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0063 and Shew_0064 are separated by 20 nucleotides Shew_0064 and Shew_0065 are separated by 68 nucleotides Shew_0065 and Shew_0066 are separated by 274 nucleotides Shew_0066 and Shew_0067 are separated by 1 nucleotides
Shew_0063: Shew_0063 - molybdopterin oxidoreductase (RefSeq), at 75,104 to 77,959
_0063
Shew_0064: Shew_0064 - 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein (RefSeq), at 77,980 to 78,549
_0064
Shew_0065: Shew_0065 - formate dehydrogenase, gamma subunit (RefSeq), at 78,618 to 79,610
_0065
Shew_0066: Shew_0066 - hypothetical protein (RefSeq), at 79,885 to 80,250
_0066
Shew_0067: Shew_0067 - hypothetical protein (RefSeq), at 80,252 to 80,761
_0067
Position (kb) 78
79
80 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 77.621 kb on - strand, within Shew_0063 at 77.751 kb on - strand at 77.794 kb on - strand at 77.882 kb on + strand at 77.928 kb on + strand at 78.067 kb on + strand, within Shew_0064 at 78.075 kb on - strand, within Shew_0064 at 78.138 kb on - strand, within Shew_0064 at 78.265 kb on + strand, within Shew_0064 at 78.265 kb on + strand, within Shew_0064 at 78.372 kb on - strand, within Shew_0064 at 78.519 kb on - strand at 78.568 kb on - strand at 78.579 kb on - strand at 78.712 kb on + strand at 78.817 kb on + strand, within Shew_0065 at 78.853 kb on + strand, within Shew_0065 at 79.008 kb on - strand, within Shew_0065 at 79.008 kb on - strand, within Shew_0065 at 79.038 kb on + strand, within Shew_0065 at 79.106 kb on + strand, within Shew_0065 at 79.114 kb on + strand, within Shew_0065 at 79.259 kb on + strand, within Shew_0065 at 79.267 kb on - strand, within Shew_0065 at 79.414 kb on + strand, within Shew_0065 at 79.538 kb on - strand at 79.717 kb on - strand at 79.744 kb on + strand at 79.758 kb on - strand at 80.040 kb on + strand, within Shew_0066 at 80.040 kb on + strand, within Shew_0066 at 80.048 kb on - strand, within Shew_0066 at 80.110 kb on + strand, within Shew_0066 at 80.118 kb on - strand, within Shew_0066 at 80.118 kb on - strand, within Shew_0066 at 80.209 kb on - strand, within Shew_0066
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM remove 77,621 - Shew_0063 0.88 +0.0 77,751 - -0.0 77,794 - -0.1 77,882 + -1.6 77,928 + +0.1 78,067 + Shew_0064 0.15 +0.3 78,075 - Shew_0064 0.17 -0.5 78,138 - Shew_0064 0.28 -1.3 78,265 + Shew_0064 0.50 +0.2 78,265 + Shew_0064 0.50 -0.0 78,372 - Shew_0064 0.69 +0.1 78,519 - -0.8 78,568 - -0.2 78,579 - -0.5 78,712 + -0.5 78,817 + Shew_0065 0.20 -3.2 78,853 + Shew_0065 0.24 +0.2 79,008 - Shew_0065 0.39 +0.1 79,008 - Shew_0065 0.39 -0.5 79,038 + Shew_0065 0.42 -1.7 79,106 + Shew_0065 0.49 +1.1 79,114 + Shew_0065 0.50 -3.1 79,259 + Shew_0065 0.65 +0.7 79,267 - Shew_0065 0.65 +0.4 79,414 + Shew_0065 0.80 -0.0 79,538 - -3.8 79,717 - -1.0 79,744 + -0.2 79,758 - -2.4 80,040 + Shew_0066 0.42 +0.3 80,040 + Shew_0066 0.42 +0.1 80,048 - Shew_0066 0.45 +1.1 80,110 + Shew_0066 0.61 +1.1 80,118 - Shew_0066 0.64 +0.4 80,118 - Shew_0066 0.64 +1.1 80,209 - Shew_0066 0.89 -0.0
Or see this region's nucleotide sequence