Experiment: BHIS with Benzethonium chloride 0.002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1640 and BT1641 are separated by 199 nucleotides BT1641 and BT1642 are separated by 113 nucleotides BT1642 and BT1643 are separated by 93 nucleotides BT1643 and BT1644 are separated by 3 nucleotides BT1644 and BT1645 are separated by 28 nucleotides
BT1640: BT1640 - hypothetical protein (NCBI ptt file), at 2,030,958 to 2,031,458
BT1640
BT1641: BT1641 - N-acetylmuramoyl-L-alanine amidase (NCBI ptt file), at 2,031,658 to 2,032,071
BT1641
BT1642: BT1642 - conserved hypothetical protein (NCBI ptt file), at 2,032,185 to 2,033,750
BT1642
BT1643: BT1643 - hypothetical protein (NCBI ptt file), at 2,033,844 to 2,034,029
BT1643
BT1644: BT1644 - putative CPS biosynthesis glycosyltransferase (NCBI ptt file), at 2,034,033 to 2,034,659
BT1644
BT1645: BT1645 - putative glycosyltransferase (NCBI ptt file), at 2,034,688 to 2,035,497
BT1645
Position (kb)
2032
2033
2034 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2031.266 kb on + strand, within BT1640 at 2031.266 kb on + strand, within BT1640 at 2031.414 kb on - strand at 2031.638 kb on - strand at 2031.924 kb on + strand, within BT1641 at 2031.924 kb on + strand, within BT1641 at 2031.925 kb on - strand, within BT1641 at 2031.956 kb on + strand, within BT1641 at 2032.049 kb on + strand at 2032.050 kb on - strand at 2032.057 kb on + strand at 2032.069 kb on + strand at 2032.070 kb on - strand at 2032.217 kb on + strand at 2032.218 kb on - strand at 2032.218 kb on - strand at 2032.352 kb on + strand, within BT1642 at 2032.354 kb on + strand, within BT1642 at 2032.362 kb on - strand, within BT1642 at 2032.407 kb on + strand, within BT1642 at 2032.408 kb on - strand, within BT1642 at 2032.424 kb on - strand, within BT1642 at 2032.443 kb on + strand, within BT1642 at 2032.444 kb on - strand, within BT1642 at 2032.449 kb on - strand, within BT1642 at 2032.451 kb on - strand, within BT1642 at 2032.455 kb on + strand, within BT1642 at 2032.612 kb on - strand, within BT1642 at 2032.616 kb on - strand, within BT1642 at 2032.676 kb on + strand, within BT1642 at 2032.745 kb on + strand, within BT1642 at 2032.746 kb on - strand, within BT1642 at 2032.819 kb on - strand, within BT1642 at 2032.819 kb on - strand, within BT1642 at 2032.966 kb on + strand, within BT1642 at 2032.990 kb on - strand, within BT1642 at 2033.052 kb on - strand, within BT1642 at 2033.163 kb on - strand, within BT1642 at 2033.333 kb on - strand, within BT1642 at 2033.333 kb on - strand, within BT1642 at 2033.399 kb on + strand, within BT1642 at 2033.427 kb on + strand, within BT1642 at 2033.445 kb on - strand, within BT1642 at 2033.500 kb on - strand, within BT1642 at 2033.572 kb on - strand, within BT1642 at 2033.583 kb on - strand at 2033.624 kb on + strand at 2033.625 kb on - strand at 2033.626 kb on + strand at 2033.627 kb on - strand at 2033.627 kb on - strand at 2033.659 kb on + strand at 2033.673 kb on + strand at 2033.731 kb on - strand at 2033.758 kb on - strand at 2033.825 kb on - strand at 2033.900 kb on + strand, within BT1643 at 2033.954 kb on + strand, within BT1643 at 2033.991 kb on - strand, within BT1643 at 2033.991 kb on - strand, within BT1643 at 2034.061 kb on - strand at 2034.158 kb on + strand, within BT1644 at 2034.159 kb on - strand, within BT1644 at 2034.182 kb on + strand, within BT1644 at 2034.182 kb on + strand, within BT1644 at 2034.188 kb on - strand, within BT1644 at 2034.255 kb on + strand, within BT1644 at 2034.297 kb on - strand, within BT1644 at 2034.396 kb on - strand, within BT1644 at 2034.396 kb on - strand, within BT1644 at 2034.530 kb on + strand, within BT1644 at 2034.531 kb on - strand, within BT1644 at 2034.532 kb on + strand, within BT1644 at 2034.659 kb on + strand at 2034.665 kb on - strand at 2034.718 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Benzethonium chloride 0.002 mM remove 2,031,266 + BT1640 0.61 +0.4 2,031,266 + BT1640 0.61 -0.1 2,031,414 - +0.0 2,031,638 - +0.9 2,031,924 + BT1641 0.64 +0.5 2,031,924 + BT1641 0.64 -1.0 2,031,925 - BT1641 0.64 -1.3 2,031,956 + BT1641 0.72 -0.2 2,032,049 + -1.8 2,032,050 - -1.0 2,032,057 + +0.1 2,032,069 + -1.5 2,032,070 - +1.0 2,032,217 + -1.6 2,032,218 - +0.4 2,032,218 - -0.0 2,032,352 + BT1642 0.11 -1.0 2,032,354 + BT1642 0.11 -1.3 2,032,362 - BT1642 0.11 -0.6 2,032,407 + BT1642 0.14 -1.4 2,032,408 - BT1642 0.14 +0.5 2,032,424 - BT1642 0.15 +0.6 2,032,443 + BT1642 0.16 +0.7 2,032,444 - BT1642 0.17 -2.0 2,032,449 - BT1642 0.17 -0.4 2,032,451 - BT1642 0.17 -1.0 2,032,455 + BT1642 0.17 +0.9 2,032,612 - BT1642 0.27 -0.7 2,032,616 - BT1642 0.28 -1.2 2,032,676 + BT1642 0.31 -1.0 2,032,745 + BT1642 0.36 +0.6 2,032,746 - BT1642 0.36 +0.1 2,032,819 - BT1642 0.40 -1.5 2,032,819 - BT1642 0.40 -0.4 2,032,966 + BT1642 0.50 -0.4 2,032,990 - BT1642 0.51 -0.0 2,033,052 - BT1642 0.55 -1.0 2,033,163 - BT1642 0.62 +2.1 2,033,333 - BT1642 0.73 -0.1 2,033,333 - BT1642 0.73 -1.8 2,033,399 + BT1642 0.78 -2.4 2,033,427 + BT1642 0.79 -0.4 2,033,445 - BT1642 0.80 -2.9 2,033,500 - BT1642 0.84 -0.3 2,033,572 - BT1642 0.89 -2.6 2,033,583 - +0.6 2,033,624 + -0.2 2,033,625 - -0.4 2,033,626 + -1.4 2,033,627 - -0.3 2,033,627 - +0.4 2,033,659 + +0.8 2,033,673 + -1.8 2,033,731 - -0.3 2,033,758 - -1.9 2,033,825 - -1.8 2,033,900 + BT1643 0.30 -0.4 2,033,954 + BT1643 0.59 -0.3 2,033,991 - BT1643 0.79 +1.0 2,033,991 - BT1643 0.79 -2.4 2,034,061 - -3.2 2,034,158 + BT1644 0.20 -0.4 2,034,159 - BT1644 0.20 -1.8 2,034,182 + BT1644 0.24 -0.6 2,034,182 + BT1644 0.24 -0.4 2,034,188 - BT1644 0.25 -0.4 2,034,255 + BT1644 0.35 +1.3 2,034,297 - BT1644 0.42 -0.7 2,034,396 - BT1644 0.58 -0.9 2,034,396 - BT1644 0.58 +1.0 2,034,530 + BT1644 0.79 -1.1 2,034,531 - BT1644 0.79 -0.2 2,034,532 + BT1644 0.80 +0.1 2,034,659 + +1.2 2,034,665 - +0.8 2,034,718 - -2.8
Or see this region's nucleotide sequence