Experiment: Formate N-free with 2mM Adenosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08905 and MMJJ_RS08910 are separated by 22 nucleotides MMJJ_RS08910 and MMJJ_RS08915 are separated by 131 nucleotides MMJJ_RS08915 and MMJJ_RS08920 are separated by 34 nucleotides MMJJ_RS08920 and MMJJ_RS08925 are separated by 46 nucleotides
MMJJ_RS08905: MMJJ_RS08905 - YcaO-related McrA-glycine thioamidation protein, at 1,642,889 to 1,644,091
_RS08905
MMJJ_RS08910: MMJJ_RS08910 - DUF167 domain-containing protein, at 1,644,114 to 1,644,419
_RS08910
MMJJ_RS08915: MMJJ_RS08915 - CoB--CoM heterodisulfide reductase subunit C, at 1,644,551 to 1,645,105
_RS08915
MMJJ_RS08920: MMJJ_RS08920 - CoB--CoM heterodisulfide reductase subunit B, at 1,645,140 to 1,646,021
_RS08920
MMJJ_RS08925: MMJJ_RS08925 - winged helix-turn-helix transcriptional regulator, at 1,646,068 to 1,646,862
_RS08925
Position (kb)
1644
1645
1646 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1643.687 kb on + strand, within MMJJ_RS08905 at 1643.687 kb on + strand, within MMJJ_RS08905 at 1643.687 kb on + strand, within MMJJ_RS08905 at 1643.719 kb on - strand, within MMJJ_RS08905 at 1643.750 kb on - strand, within MMJJ_RS08905 at 1643.750 kb on - strand, within MMJJ_RS08905 at 1643.751 kb on + strand, within MMJJ_RS08905 at 1643.792 kb on - strand, within MMJJ_RS08905 at 1643.898 kb on - strand, within MMJJ_RS08905 at 1643.898 kb on - strand, within MMJJ_RS08905 at 1643.898 kb on - strand, within MMJJ_RS08905 at 1643.898 kb on - strand, within MMJJ_RS08905 at 1643.899 kb on + strand, within MMJJ_RS08905 at 1643.899 kb on + strand, within MMJJ_RS08905 at 1643.966 kb on - strand, within MMJJ_RS08905 at 1643.966 kb on - strand, within MMJJ_RS08905 at 1643.966 kb on - strand, within MMJJ_RS08905 at 1643.967 kb on + strand, within MMJJ_RS08905 at 1644.008 kb on - strand at 1644.009 kb on + strand at 1644.011 kb on - strand at 1644.055 kb on - strand at 1644.055 kb on - strand at 1644.055 kb on - strand at 1644.055 kb on - strand at 1644.056 kb on + strand at 1644.056 kb on + strand at 1644.056 kb on + strand at 1644.058 kb on + strand at 1644.072 kb on - strand at 1644.072 kb on - strand at 1644.076 kb on - strand at 1644.076 kb on - strand at 1644.076 kb on - strand at 1644.077 kb on + strand at 1644.077 kb on + strand at 1644.102 kb on - strand at 1644.103 kb on + strand at 1644.125 kb on - strand at 1644.126 kb on + strand at 1644.126 kb on + strand at 1644.147 kb on - strand, within MMJJ_RS08910 at 1644.167 kb on - strand, within MMJJ_RS08910 at 1644.176 kb on - strand, within MMJJ_RS08910 at 1644.176 kb on - strand, within MMJJ_RS08910 at 1644.176 kb on - strand, within MMJJ_RS08910 at 1644.176 kb on - strand, within MMJJ_RS08910 at 1644.176 kb on - strand, within MMJJ_RS08910 at 1644.176 kb on - strand, within MMJJ_RS08910 at 1644.176 kb on - strand, within MMJJ_RS08910 at 1644.177 kb on + strand, within MMJJ_RS08910 at 1644.177 kb on + strand, within MMJJ_RS08910 at 1644.177 kb on + strand, within MMJJ_RS08910 at 1644.177 kb on + strand, within MMJJ_RS08910 at 1644.180 kb on - strand, within MMJJ_RS08910 at 1644.181 kb on + strand, within MMJJ_RS08910 at 1644.219 kb on - strand, within MMJJ_RS08910 at 1644.219 kb on - strand, within MMJJ_RS08910 at 1644.220 kb on + strand, within MMJJ_RS08910 at 1644.253 kb on + strand, within MMJJ_RS08910 at 1644.293 kb on - strand, within MMJJ_RS08910 at 1644.301 kb on + strand, within MMJJ_RS08910 at 1644.306 kb on - strand, within MMJJ_RS08910 at 1644.359 kb on - strand, within MMJJ_RS08910 at 1644.360 kb on + strand, within MMJJ_RS08910 at 1644.360 kb on + strand, within MMJJ_RS08910 at 1644.362 kb on - strand, within MMJJ_RS08910 at 1644.379 kb on + strand, within MMJJ_RS08910 at 1644.433 kb on - strand at 1644.434 kb on + strand at 1644.438 kb on + strand at 1644.440 kb on - strand at 1644.455 kb on - strand at 1644.456 kb on + strand at 1644.456 kb on + strand at 1644.495 kb on + strand at 1644.507 kb on - strand at 1644.511 kb on - strand at 1644.511 kb on - strand at 1644.511 kb on - strand at 1644.511 kb on - strand at 1644.511 kb on - strand at 1644.511 kb on - strand at 1644.512 kb on + strand at 1644.512 kb on + strand at 1644.513 kb on - strand at 1644.516 kb on - strand at 1644.516 kb on - strand at 1644.516 kb on - strand at 1644.516 kb on - strand at 1644.516 kb on - strand at 1644.517 kb on + strand at 1644.518 kb on - strand at 1644.518 kb on - strand at 1644.518 kb on - strand at 1644.518 kb on - strand at 1644.518 kb on - strand at 1644.519 kb on + strand at 1644.519 kb on + strand at 1644.519 kb on + strand at 1644.520 kb on - strand at 1644.520 kb on - strand at 1644.521 kb on + strand at 1644.521 kb on + strand at 1644.521 kb on + strand at 1644.670 kb on - strand, within MMJJ_RS08915 at 1644.723 kb on - strand, within MMJJ_RS08915 at 1644.723 kb on - strand, within MMJJ_RS08915 at 1644.724 kb on + strand, within MMJJ_RS08915 at 1644.781 kb on - strand, within MMJJ_RS08915 at 1644.906 kb on - strand, within MMJJ_RS08915 at 1644.906 kb on - strand, within MMJJ_RS08915 at 1644.907 kb on + strand, within MMJJ_RS08915 at 1644.907 kb on + strand, within MMJJ_RS08915 at 1644.907 kb on + strand, within MMJJ_RS08915 at 1644.907 kb on + strand, within MMJJ_RS08915 at 1644.908 kb on - strand, within MMJJ_RS08915 at 1644.908 kb on - strand, within MMJJ_RS08915 at 1644.909 kb on + strand, within MMJJ_RS08915 at 1644.916 kb on + strand, within MMJJ_RS08915 at 1644.916 kb on + strand, within MMJJ_RS08915 at 1645.108 kb on - strand at 1645.109 kb on + strand at 1645.124 kb on - strand at 1645.178 kb on - strand at 1645.179 kb on + strand at 1645.179 kb on + strand at 1645.203 kb on + strand at 1645.241 kb on + strand, within MMJJ_RS08920 at 1645.359 kb on + strand, within MMJJ_RS08920 at 1645.359 kb on + strand, within MMJJ_RS08920 at 1645.370 kb on + strand, within MMJJ_RS08920 at 1645.370 kb on + strand, within MMJJ_RS08920 at 1645.370 kb on + strand, within MMJJ_RS08920 at 1645.414 kb on - strand, within MMJJ_RS08920 at 1645.495 kb on - strand, within MMJJ_RS08920 at 1645.495 kb on - strand, within MMJJ_RS08920 at 1645.559 kb on + strand, within MMJJ_RS08920 at 1645.567 kb on - strand, within MMJJ_RS08920 at 1645.606 kb on - strand, within MMJJ_RS08920 at 1645.685 kb on - strand, within MMJJ_RS08920 at 1645.686 kb on + strand, within MMJJ_RS08920 at 1645.693 kb on - strand, within MMJJ_RS08920 at 1645.698 kb on - strand, within MMJJ_RS08920 at 1645.698 kb on - strand, within MMJJ_RS08920 at 1645.699 kb on + strand, within MMJJ_RS08920 at 1645.699 kb on + strand, within MMJJ_RS08920 at 1645.699 kb on + strand, within MMJJ_RS08920 at 1645.706 kb on - strand, within MMJJ_RS08920 at 1645.707 kb on + strand, within MMJJ_RS08920 at 1645.739 kb on + strand, within MMJJ_RS08920 at 1645.739 kb on + strand, within MMJJ_RS08920 at 1645.739 kb on + strand, within MMJJ_RS08920 at 1645.746 kb on + strand, within MMJJ_RS08920 at 1645.804 kb on - strand, within MMJJ_RS08920 at 1645.804 kb on - strand, within MMJJ_RS08920 at 1645.828 kb on - strand, within MMJJ_RS08920 at 1645.828 kb on - strand, within MMJJ_RS08920 at 1645.828 kb on - strand, within MMJJ_RS08920 at 1645.828 kb on - strand, within MMJJ_RS08920 at 1645.828 kb on - strand, within MMJJ_RS08920 at 1645.829 kb on + strand, within MMJJ_RS08920 at 1645.829 kb on + strand, within MMJJ_RS08920 at 1645.829 kb on + strand, within MMJJ_RS08920 at 1645.829 kb on + strand, within MMJJ_RS08920 at 1645.829 kb on + strand, within MMJJ_RS08920 at 1645.846 kb on - strand, within MMJJ_RS08920 at 1645.847 kb on + strand, within MMJJ_RS08920 at 1645.847 kb on + strand, within MMJJ_RS08920 at 1645.886 kb on + strand, within MMJJ_RS08920 at 1645.896 kb on - strand, within MMJJ_RS08920 at 1645.912 kb on - strand, within MMJJ_RS08920 at 1645.912 kb on - strand, within MMJJ_RS08920 at 1645.912 kb on - strand, within MMJJ_RS08920 at 1645.913 kb on + strand, within MMJJ_RS08920 at 1645.913 kb on + strand, within MMJJ_RS08920 at 1645.913 kb on + strand, within MMJJ_RS08920 at 1645.913 kb on + strand, within MMJJ_RS08920 at 1645.913 kb on + strand, within MMJJ_RS08920 at 1645.918 kb on - strand, within MMJJ_RS08920 at 1645.918 kb on - strand, within MMJJ_RS08920 at 1645.918 kb on - strand, within MMJJ_RS08920 at 1645.919 kb on + strand, within MMJJ_RS08920 at 1645.931 kb on + strand, within MMJJ_RS08920 at 1645.976 kb on + strand at 1645.976 kb on + strand at 1645.982 kb on - strand at 1645.982 kb on - strand at 1645.999 kb on - strand at 1645.999 kb on - strand at 1646.016 kb on - strand at 1646.017 kb on + strand at 1646.019 kb on - strand at 1646.019 kb on - strand at 1646.062 kb on - strand at 1646.063 kb on + strand at 1646.063 kb on + strand at 1646.065 kb on - strand at 1646.069 kb on - strand at 1646.069 kb on - strand at 1646.069 kb on - strand at 1646.101 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate N-free with 2mM Adenosine remove 1,643,687 + MMJJ_RS08905 0.66 +0.2 1,643,687 + MMJJ_RS08905 0.66 +2.9 1,643,687 + MMJJ_RS08905 0.66 -1.3 1,643,719 - MMJJ_RS08905 0.69 -2.2 1,643,750 - MMJJ_RS08905 0.72 +0.8 1,643,750 - MMJJ_RS08905 0.72 -1.5 1,643,751 + MMJJ_RS08905 0.72 -0.2 1,643,792 - MMJJ_RS08905 0.75 +2.6 1,643,898 - MMJJ_RS08905 0.84 +1.9 1,643,898 - MMJJ_RS08905 0.84 -0.2 1,643,898 - MMJJ_RS08905 0.84 -0.9 1,643,898 - MMJJ_RS08905 0.84 +0.8 1,643,899 + MMJJ_RS08905 0.84 -2.4 1,643,899 + MMJJ_RS08905 0.84 -0.9 1,643,966 - MMJJ_RS08905 0.90 -0.7 1,643,966 - MMJJ_RS08905 0.90 +0.6 1,643,966 - MMJJ_RS08905 0.90 +3.3 1,643,967 + MMJJ_RS08905 0.90 +4.2 1,644,008 - -1.8 1,644,009 + -0.3 1,644,011 - +0.3 1,644,055 - +0.7 1,644,055 - -1.2 1,644,055 - +1.8 1,644,055 - -0.7 1,644,056 + +2.6 1,644,056 + +0.7 1,644,056 + +1.6 1,644,058 + -0.1 1,644,072 - -1.2 1,644,072 - -2.7 1,644,076 - -0.2 1,644,076 - +1.6 1,644,076 - -0.5 1,644,077 + -1.2 1,644,077 + -0.6 1,644,102 - +2.6 1,644,103 + -1.4 1,644,125 - +2.8 1,644,126 + +2.1 1,644,126 + +0.4 1,644,147 - MMJJ_RS08910 0.11 -2.5 1,644,167 - MMJJ_RS08910 0.17 -0.1 1,644,176 - MMJJ_RS08910 0.20 +1.1 1,644,176 - MMJJ_RS08910 0.20 -0.6 1,644,176 - MMJJ_RS08910 0.20 +1.5 1,644,176 - MMJJ_RS08910 0.20 +2.1 1,644,176 - MMJJ_RS08910 0.20 +1.7 1,644,176 - MMJJ_RS08910 0.20 +5.4 1,644,176 - MMJJ_RS08910 0.20 +4.1 1,644,177 + MMJJ_RS08910 0.21 -1.2 1,644,177 + MMJJ_RS08910 0.21 +0.9 1,644,177 + MMJJ_RS08910 0.21 +0.3 1,644,177 + MMJJ_RS08910 0.21 -2.2 1,644,180 - MMJJ_RS08910 0.22 +0.6 1,644,181 + MMJJ_RS08910 0.22 +1.8 1,644,219 - MMJJ_RS08910 0.34 -1.0 1,644,219 - MMJJ_RS08910 0.34 -0.2 1,644,220 + MMJJ_RS08910 0.35 -1.5 1,644,253 + MMJJ_RS08910 0.45 -2.1 1,644,293 - MMJJ_RS08910 0.58 +1.9 1,644,301 + MMJJ_RS08910 0.61 +0.5 1,644,306 - MMJJ_RS08910 0.63 -0.3 1,644,359 - MMJJ_RS08910 0.80 +3.2 1,644,360 + MMJJ_RS08910 0.80 +1.2 1,644,360 + MMJJ_RS08910 0.80 +1.1 1,644,362 - MMJJ_RS08910 0.81 -0.9 1,644,379 + MMJJ_RS08910 0.87 -2.2 1,644,433 - +0.3 1,644,434 + -3.2 1,644,438 + +2.5 1,644,440 - +0.9 1,644,455 - -0.3 1,644,456 + +2.8 1,644,456 + +3.3 1,644,495 + -3.4 1,644,507 - -2.1 1,644,511 - -2.1 1,644,511 - +0.0 1,644,511 - -3.0 1,644,511 - -2.2 1,644,511 - -2.7 1,644,511 - +0.3 1,644,512 + +0.5 1,644,512 + -2.2 1,644,513 - -1.1 1,644,516 - -1.9 1,644,516 - -1.3 1,644,516 - -0.7 1,644,516 - -2.3 1,644,516 - -2.2 1,644,517 + -1.2 1,644,518 - -4.5 1,644,518 - -3.2 1,644,518 - -3.2 1,644,518 - -1.5 1,644,518 - +0.8 1,644,519 + -3.3 1,644,519 + -1.0 1,644,519 + -2.9 1,644,520 - -0.7 1,644,520 - -2.4 1,644,521 + +0.6 1,644,521 + -1.2 1,644,521 + -1.5 1,644,670 - MMJJ_RS08915 0.21 -1.2 1,644,723 - MMJJ_RS08915 0.31 -1.8 1,644,723 - MMJJ_RS08915 0.31 -1.9 1,644,724 + MMJJ_RS08915 0.31 -3.8 1,644,781 - MMJJ_RS08915 0.41 -2.1 1,644,906 - MMJJ_RS08915 0.64 -3.3 1,644,906 - MMJJ_RS08915 0.64 -3.7 1,644,907 + MMJJ_RS08915 0.64 -1.2 1,644,907 + MMJJ_RS08915 0.64 -3.2 1,644,907 + MMJJ_RS08915 0.64 -1.4 1,644,907 + MMJJ_RS08915 0.64 -2.5 1,644,908 - MMJJ_RS08915 0.64 -3.4 1,644,908 - MMJJ_RS08915 0.64 -0.7 1,644,909 + MMJJ_RS08915 0.65 -1.9 1,644,916 + MMJJ_RS08915 0.66 -3.3 1,644,916 + MMJJ_RS08915 0.66 -2.9 1,645,108 - -1.7 1,645,109 + -3.8 1,645,124 - -0.5 1,645,178 - -2.3 1,645,179 + -2.1 1,645,179 + -2.0 1,645,203 + -2.9 1,645,241 + MMJJ_RS08920 0.11 -1.9 1,645,359 + MMJJ_RS08920 0.25 -1.9 1,645,359 + MMJJ_RS08920 0.25 -1.2 1,645,370 + MMJJ_RS08920 0.26 -2.2 1,645,370 + MMJJ_RS08920 0.26 -2.7 1,645,370 + MMJJ_RS08920 0.26 -2.0 1,645,414 - MMJJ_RS08920 0.31 -3.6 1,645,495 - MMJJ_RS08920 0.40 -2.2 1,645,495 - MMJJ_RS08920 0.40 -0.2 1,645,559 + MMJJ_RS08920 0.48 -1.2 1,645,567 - MMJJ_RS08920 0.48 -2.9 1,645,606 - MMJJ_RS08920 0.53 -1.1 1,645,685 - MMJJ_RS08920 0.62 -1.4 1,645,686 + MMJJ_RS08920 0.62 +0.1 1,645,693 - MMJJ_RS08920 0.63 -0.0 1,645,698 - MMJJ_RS08920 0.63 -2.5 1,645,698 - MMJJ_RS08920 0.63 -3.5 1,645,699 + MMJJ_RS08920 0.63 -1.1 1,645,699 + MMJJ_RS08920 0.63 -2.5 1,645,699 + MMJJ_RS08920 0.63 -1.3 1,645,706 - MMJJ_RS08920 0.64 -2.3 1,645,707 + MMJJ_RS08920 0.64 -3.0 1,645,739 + MMJJ_RS08920 0.68 -1.6 1,645,739 + MMJJ_RS08920 0.68 +0.8 1,645,739 + MMJJ_RS08920 0.68 -3.3 1,645,746 + MMJJ_RS08920 0.69 -2.7 1,645,804 - MMJJ_RS08920 0.75 -3.1 1,645,804 - MMJJ_RS08920 0.75 -2.6 1,645,828 - MMJJ_RS08920 0.78 -3.2 1,645,828 - MMJJ_RS08920 0.78 -3.0 1,645,828 - MMJJ_RS08920 0.78 -3.5 1,645,828 - MMJJ_RS08920 0.78 -0.2 1,645,828 - MMJJ_RS08920 0.78 -1.0 1,645,829 + MMJJ_RS08920 0.78 -1.3 1,645,829 + MMJJ_RS08920 0.78 -2.5 1,645,829 + MMJJ_RS08920 0.78 -1.7 1,645,829 + MMJJ_RS08920 0.78 -2.5 1,645,829 + MMJJ_RS08920 0.78 -0.1 1,645,846 - MMJJ_RS08920 0.80 -0.6 1,645,847 + MMJJ_RS08920 0.80 -3.4 1,645,847 + MMJJ_RS08920 0.80 -1.4 1,645,886 + MMJJ_RS08920 0.85 -4.4 1,645,896 - MMJJ_RS08920 0.86 -1.7 1,645,912 - MMJJ_RS08920 0.88 -2.2 1,645,912 - MMJJ_RS08920 0.88 -2.0 1,645,912 - MMJJ_RS08920 0.88 -2.6 1,645,913 + MMJJ_RS08920 0.88 -3.7 1,645,913 + MMJJ_RS08920 0.88 -1.0 1,645,913 + MMJJ_RS08920 0.88 -1.5 1,645,913 + MMJJ_RS08920 0.88 -4.3 1,645,913 + MMJJ_RS08920 0.88 -2.9 1,645,918 - MMJJ_RS08920 0.88 -0.9 1,645,918 - MMJJ_RS08920 0.88 -3.7 1,645,918 - MMJJ_RS08920 0.88 -2.4 1,645,919 + MMJJ_RS08920 0.88 -1.1 1,645,931 + MMJJ_RS08920 0.90 -2.2 1,645,976 + +2.1 1,645,976 + -1.8 1,645,982 - -2.9 1,645,982 - +1.2 1,645,999 - +1.6 1,645,999 - -2.0 1,646,016 - +2.5 1,646,017 + +3.2 1,646,019 - -0.8 1,646,019 - +1.4 1,646,062 - +0.8 1,646,063 + +3.4 1,646,063 + +1.2 1,646,065 - +0.6 1,646,069 - -1.4 1,646,069 - +0.2 1,646,069 - +3.8 1,646,101 - -1.0
Or see this region's nucleotide sequence