Experiment: Formate N-free with 2mM Adenosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06130 and MMJJ_RS06135 are separated by 115 nucleotides MMJJ_RS06135 and MMJJ_RS06140 are separated by 27 nucleotides MMJJ_RS06140 and MMJJ_RS06145 are separated by 30 nucleotides MMJJ_RS06145 and MMJJ_RS06150 are separated by 15 nucleotides
MMJJ_RS06130: MMJJ_RS06130 - hypothetical protein, at 1,138,628 to 1,138,855
_RS06130
MMJJ_RS06135: MMJJ_RS06135 - 50S ribosomal protein L11 methyltransferase, at 1,138,971 to 1,139,753
_RS06135
MMJJ_RS06140: MMJJ_RS06140 - RimK family alpha-L-glutamate ligase, at 1,139,781 to 1,140,587
_RS06140
MMJJ_RS06145: MMJJ_RS06145 - TrkA family potassium uptake protein, at 1,140,618 to 1,141,283
_RS06145
MMJJ_RS06150: MMJJ_RS06150 - DUF483 domain-containing protein, at 1,141,299 to 1,141,853
_RS06150
Position (kb)
1139
1140
1141 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 1138.812 kb on + strand, within MMJJ_RS06130 at 1138.822 kb on - strand, within MMJJ_RS06130 at 1138.823 kb on + strand, within MMJJ_RS06130 at 1138.823 kb on + strand, within MMJJ_RS06130 at 1138.835 kb on - strand at 1138.838 kb on - strand at 1138.838 kb on - strand at 1138.839 kb on + strand at 1138.839 kb on + strand at 1138.920 kb on - strand at 1138.920 kb on - strand at 1138.920 kb on - strand at 1138.921 kb on + strand at 1138.921 kb on + strand at 1138.923 kb on - strand at 1138.923 kb on - strand at 1138.923 kb on - strand at 1138.923 kb on - strand at 1138.924 kb on + strand at 1138.924 kb on + strand at 1138.924 kb on + strand at 1138.925 kb on - strand at 1138.925 kb on - strand at 1138.925 kb on - strand at 1138.925 kb on - strand at 1138.925 kb on - strand at 1138.925 kb on - strand at 1138.926 kb on + strand at 1138.926 kb on + strand at 1138.926 kb on + strand at 1138.927 kb on - strand at 1138.927 kb on - strand at 1138.927 kb on - strand at 1138.927 kb on - strand at 1138.928 kb on + strand at 1138.928 kb on + strand at 1138.928 kb on + strand at 1138.928 kb on + strand at 1138.928 kb on + strand at 1138.928 kb on + strand at 1138.928 kb on + strand at 1138.928 kb on + strand at 1138.931 kb on - strand at 1138.931 kb on - strand at 1138.937 kb on - strand at 1138.938 kb on + strand at 1138.966 kb on - strand at 1138.969 kb on - strand at 1138.970 kb on + strand at 1138.970 kb on + strand at 1138.978 kb on - strand at 1138.990 kb on - strand at 1138.991 kb on + strand at 1139.038 kb on - strand at 1139.083 kb on - strand, within MMJJ_RS06135 at 1139.084 kb on + strand, within MMJJ_RS06135 at 1139.084 kb on + strand, within MMJJ_RS06135 at 1139.084 kb on + strand, within MMJJ_RS06135 at 1139.182 kb on + strand, within MMJJ_RS06135 at 1139.184 kb on + strand, within MMJJ_RS06135 at 1139.197 kb on - strand, within MMJJ_RS06135 at 1139.197 kb on - strand, within MMJJ_RS06135 at 1139.198 kb on + strand, within MMJJ_RS06135 at 1139.247 kb on - strand, within MMJJ_RS06135 at 1139.247 kb on - strand, within MMJJ_RS06135 at 1139.247 kb on - strand, within MMJJ_RS06135 at 1139.247 kb on - strand, within MMJJ_RS06135 at 1139.247 kb on - strand, within MMJJ_RS06135 at 1139.247 kb on - strand, within MMJJ_RS06135 at 1139.247 kb on - strand, within MMJJ_RS06135 at 1139.248 kb on + strand, within MMJJ_RS06135 at 1139.248 kb on + strand, within MMJJ_RS06135 at 1139.250 kb on - strand, within MMJJ_RS06135 at 1139.251 kb on + strand, within MMJJ_RS06135 at 1139.305 kb on - strand, within MMJJ_RS06135 at 1139.305 kb on - strand, within MMJJ_RS06135 at 1139.306 kb on + strand, within MMJJ_RS06135 at 1139.332 kb on - strand, within MMJJ_RS06135 at 1139.359 kb on - strand, within MMJJ_RS06135 at 1139.377 kb on - strand, within MMJJ_RS06135 at 1139.394 kb on - strand, within MMJJ_RS06135 at 1139.394 kb on - strand, within MMJJ_RS06135 at 1139.394 kb on - strand, within MMJJ_RS06135 at 1139.396 kb on - strand, within MMJJ_RS06135 at 1139.411 kb on + strand, within MMJJ_RS06135 at 1139.413 kb on - strand, within MMJJ_RS06135 at 1139.414 kb on + strand, within MMJJ_RS06135 at 1139.436 kb on - strand, within MMJJ_RS06135 at 1139.437 kb on + strand, within MMJJ_RS06135 at 1139.437 kb on + strand, within MMJJ_RS06135 at 1139.439 kb on - strand, within MMJJ_RS06135 at 1139.489 kb on - strand, within MMJJ_RS06135 at 1139.489 kb on - strand, within MMJJ_RS06135 at 1139.490 kb on + strand, within MMJJ_RS06135 at 1139.533 kb on - strand, within MMJJ_RS06135 at 1139.560 kb on - strand, within MMJJ_RS06135 at 1139.589 kb on - strand, within MMJJ_RS06135 at 1139.589 kb on - strand, within MMJJ_RS06135 at 1139.589 kb on - strand, within MMJJ_RS06135 at 1139.590 kb on + strand, within MMJJ_RS06135 at 1139.590 kb on + strand, within MMJJ_RS06135 at 1139.591 kb on - strand, within MMJJ_RS06135 at 1139.594 kb on - strand, within MMJJ_RS06135 at 1139.594 kb on - strand, within MMJJ_RS06135 at 1139.595 kb on + strand, within MMJJ_RS06135 at 1139.651 kb on + strand, within MMJJ_RS06135 at 1139.651 kb on + strand, within MMJJ_RS06135 at 1139.671 kb on - strand, within MMJJ_RS06135 at 1139.693 kb on + strand at 1139.703 kb on - strand at 1139.703 kb on - strand at 1139.707 kb on - strand at 1139.708 kb on + strand at 1139.794 kb on - strand at 1139.867 kb on - strand, within MMJJ_RS06140 at 1139.879 kb on + strand, within MMJJ_RS06140 at 1139.968 kb on - strand, within MMJJ_RS06140 at 1139.968 kb on - strand, within MMJJ_RS06140 at 1140.036 kb on - strand, within MMJJ_RS06140 at 1140.037 kb on + strand, within MMJJ_RS06140 at 1140.041 kb on - strand, within MMJJ_RS06140 at 1140.041 kb on - strand, within MMJJ_RS06140 at 1140.041 kb on - strand, within MMJJ_RS06140 at 1140.042 kb on + strand, within MMJJ_RS06140 at 1140.045 kb on - strand, within MMJJ_RS06140 at 1140.128 kb on - strand, within MMJJ_RS06140 at 1140.152 kb on + strand, within MMJJ_RS06140 at 1140.194 kb on + strand, within MMJJ_RS06140 at 1140.270 kb on - strand, within MMJJ_RS06140 at 1140.270 kb on - strand, within MMJJ_RS06140 at 1140.271 kb on + strand, within MMJJ_RS06140 at 1140.271 kb on + strand, within MMJJ_RS06140 at 1140.271 kb on + strand, within MMJJ_RS06140 at 1140.271 kb on + strand, within MMJJ_RS06140 at 1140.272 kb on - strand, within MMJJ_RS06140 at 1140.272 kb on - strand, within MMJJ_RS06140 at 1140.272 kb on - strand, within MMJJ_RS06140 at 1140.272 kb on - strand, within MMJJ_RS06140 at 1140.273 kb on + strand, within MMJJ_RS06140 at 1140.273 kb on + strand, within MMJJ_RS06140 at 1140.283 kb on - strand, within MMJJ_RS06140 at 1140.283 kb on - strand, within MMJJ_RS06140 at 1140.357 kb on - strand, within MMJJ_RS06140 at 1140.358 kb on + strand, within MMJJ_RS06140 at 1140.387 kb on + strand, within MMJJ_RS06140 at 1140.391 kb on - strand, within MMJJ_RS06140 at 1140.463 kb on - strand, within MMJJ_RS06140 at 1140.466 kb on - strand, within MMJJ_RS06140 at 1140.466 kb on - strand, within MMJJ_RS06140 at 1140.487 kb on + strand, within MMJJ_RS06140 at 1140.494 kb on + strand, within MMJJ_RS06140 at 1140.538 kb on - strand at 1140.564 kb on - strand at 1140.565 kb on + strand at 1140.572 kb on - strand at 1140.572 kb on - strand at 1140.572 kb on - strand at 1140.573 kb on + strand at 1140.573 kb on + strand at 1140.573 kb on + strand at 1140.573 kb on + strand at 1140.573 kb on + strand at 1140.573 kb on + strand at 1140.573 kb on + strand at 1140.573 kb on + strand at 1140.576 kb on - strand at 1140.577 kb on + strand at 1140.580 kb on - strand at 1140.580 kb on - strand at 1140.580 kb on - strand at 1140.585 kb on - strand at 1140.643 kb on + strand at 1140.762 kb on + strand, within MMJJ_RS06145 at 1140.940 kb on + strand, within MMJJ_RS06145 at 1141.207 kb on + strand, within MMJJ_RS06145 at 1141.399 kb on - strand, within MMJJ_RS06150 at 1141.495 kb on - strand, within MMJJ_RS06150 at 1141.495 kb on - strand, within MMJJ_RS06150 at 1141.548 kb on - strand, within MMJJ_RS06150 at 1141.555 kb on - strand, within MMJJ_RS06150
Per-strain Table
Position Strand Gene LocusTag Fraction Formate N-free with 2mM Adenosine remove 1,138,812 + MMJJ_RS06130 0.81 -2.0 1,138,822 - MMJJ_RS06130 0.85 +2.5 1,138,823 + MMJJ_RS06130 0.86 +0.5 1,138,823 + MMJJ_RS06130 0.86 +0.6 1,138,835 - -0.2 1,138,838 - -0.8 1,138,838 - +0.9 1,138,839 + -1.1 1,138,839 + -1.2 1,138,920 - +1.2 1,138,920 - -0.1 1,138,920 - -0.7 1,138,921 + -0.6 1,138,921 + -0.3 1,138,923 - +3.6 1,138,923 - -0.5 1,138,923 - -0.9 1,138,923 - +2.4 1,138,924 + -3.4 1,138,924 + -1.7 1,138,924 + -1.8 1,138,925 - +5.0 1,138,925 - +4.4 1,138,925 - -1.7 1,138,925 - -0.4 1,138,925 - -0.2 1,138,925 - +1.8 1,138,926 + +2.5 1,138,926 + -1.3 1,138,926 + +4.7 1,138,927 - +1.0 1,138,927 - +1.6 1,138,927 - -0.8 1,138,927 - +4.2 1,138,928 + +2.4 1,138,928 + +2.4 1,138,928 + +0.2 1,138,928 + +3.6 1,138,928 + +3.3 1,138,928 + -0.1 1,138,928 + +0.5 1,138,928 + +3.5 1,138,931 - -2.1 1,138,931 - -0.1 1,138,937 - +1.6 1,138,938 + +3.1 1,138,966 - -3.0 1,138,969 - +3.6 1,138,970 + -2.4 1,138,970 + +4.1 1,138,978 - -0.9 1,138,990 - -0.1 1,138,991 + +4.8 1,139,038 - -0.3 1,139,083 - MMJJ_RS06135 0.14 +1.0 1,139,084 + MMJJ_RS06135 0.14 +0.5 1,139,084 + MMJJ_RS06135 0.14 +1.3 1,139,084 + MMJJ_RS06135 0.14 +1.8 1,139,182 + MMJJ_RS06135 0.27 +2.7 1,139,184 + MMJJ_RS06135 0.27 +2.8 1,139,197 - MMJJ_RS06135 0.29 -0.5 1,139,197 - MMJJ_RS06135 0.29 +2.2 1,139,198 + MMJJ_RS06135 0.29 +1.7 1,139,247 - MMJJ_RS06135 0.35 +2.5 1,139,247 - MMJJ_RS06135 0.35 -1.2 1,139,247 - MMJJ_RS06135 0.35 +2.2 1,139,247 - MMJJ_RS06135 0.35 +0.3 1,139,247 - MMJJ_RS06135 0.35 +1.6 1,139,247 - MMJJ_RS06135 0.35 +2.2 1,139,247 - MMJJ_RS06135 0.35 +4.6 1,139,248 + MMJJ_RS06135 0.35 +1.1 1,139,248 + MMJJ_RS06135 0.35 +0.2 1,139,250 - MMJJ_RS06135 0.36 +1.9 1,139,251 + MMJJ_RS06135 0.36 +2.4 1,139,305 - MMJJ_RS06135 0.43 +3.6 1,139,305 - MMJJ_RS06135 0.43 +1.3 1,139,306 + MMJJ_RS06135 0.43 +0.5 1,139,332 - MMJJ_RS06135 0.46 +4.4 1,139,359 - MMJJ_RS06135 0.50 +1.0 1,139,377 - MMJJ_RS06135 0.52 +4.3 1,139,394 - MMJJ_RS06135 0.54 +3.9 1,139,394 - MMJJ_RS06135 0.54 +3.8 1,139,394 - MMJJ_RS06135 0.54 +0.1 1,139,396 - MMJJ_RS06135 0.54 -2.7 1,139,411 + MMJJ_RS06135 0.56 +0.7 1,139,413 - MMJJ_RS06135 0.56 +2.3 1,139,414 + MMJJ_RS06135 0.57 +1.8 1,139,436 - MMJJ_RS06135 0.59 -2.4 1,139,437 + MMJJ_RS06135 0.60 +2.3 1,139,437 + MMJJ_RS06135 0.60 +3.7 1,139,439 - MMJJ_RS06135 0.60 +0.6 1,139,489 - MMJJ_RS06135 0.66 +2.0 1,139,489 - MMJJ_RS06135 0.66 -1.0 1,139,490 + MMJJ_RS06135 0.66 +0.1 1,139,533 - MMJJ_RS06135 0.72 -0.7 1,139,560 - MMJJ_RS06135 0.75 +4.3 1,139,589 - MMJJ_RS06135 0.79 -0.6 1,139,589 - MMJJ_RS06135 0.79 +7.2 1,139,589 - MMJJ_RS06135 0.79 -1.6 1,139,590 + MMJJ_RS06135 0.79 +3.2 1,139,590 + MMJJ_RS06135 0.79 +1.1 1,139,591 - MMJJ_RS06135 0.79 +0.4 1,139,594 - MMJJ_RS06135 0.80 +0.6 1,139,594 - MMJJ_RS06135 0.80 -1.1 1,139,595 + MMJJ_RS06135 0.80 +3.8 1,139,651 + MMJJ_RS06135 0.87 +0.7 1,139,651 + MMJJ_RS06135 0.87 +4.4 1,139,671 - MMJJ_RS06135 0.89 +2.6 1,139,693 + -1.2 1,139,703 - +1.9 1,139,703 - +1.2 1,139,707 - +1.3 1,139,708 + -1.9 1,139,794 - +0.1 1,139,867 - MMJJ_RS06140 0.11 -4.6 1,139,879 + MMJJ_RS06140 0.12 -1.1 1,139,968 - MMJJ_RS06140 0.23 -2.5 1,139,968 - MMJJ_RS06140 0.23 -0.5 1,140,036 - MMJJ_RS06140 0.32 +0.3 1,140,037 + MMJJ_RS06140 0.32 -0.6 1,140,041 - MMJJ_RS06140 0.32 -0.1 1,140,041 - MMJJ_RS06140 0.32 +0.0 1,140,041 - MMJJ_RS06140 0.32 -1.9 1,140,042 + MMJJ_RS06140 0.32 -3.1 1,140,045 - MMJJ_RS06140 0.33 -0.3 1,140,128 - MMJJ_RS06140 0.43 +0.7 1,140,152 + MMJJ_RS06140 0.46 -2.3 1,140,194 + MMJJ_RS06140 0.51 +0.1 1,140,270 - MMJJ_RS06140 0.61 -0.5 1,140,270 - MMJJ_RS06140 0.61 -0.8 1,140,271 + MMJJ_RS06140 0.61 -2.0 1,140,271 + MMJJ_RS06140 0.61 -1.3 1,140,271 + MMJJ_RS06140 0.61 -3.5 1,140,271 + MMJJ_RS06140 0.61 +0.4 1,140,272 - MMJJ_RS06140 0.61 -1.2 1,140,272 - MMJJ_RS06140 0.61 -0.3 1,140,272 - MMJJ_RS06140 0.61 -0.8 1,140,272 - MMJJ_RS06140 0.61 -0.5 1,140,273 + MMJJ_RS06140 0.61 -2.7 1,140,273 + MMJJ_RS06140 0.61 -2.9 1,140,283 - MMJJ_RS06140 0.62 -1.8 1,140,283 - MMJJ_RS06140 0.62 +1.8 1,140,357 - MMJJ_RS06140 0.71 -2.0 1,140,358 + MMJJ_RS06140 0.71 -2.0 1,140,387 + MMJJ_RS06140 0.75 -3.5 1,140,391 - MMJJ_RS06140 0.76 -0.9 1,140,463 - MMJJ_RS06140 0.85 -2.4 1,140,466 - MMJJ_RS06140 0.85 -0.9 1,140,466 - MMJJ_RS06140 0.85 -0.3 1,140,487 + MMJJ_RS06140 0.87 -2.8 1,140,494 + MMJJ_RS06140 0.88 -1.7 1,140,538 - -0.7 1,140,564 - +0.4 1,140,565 + -0.9 1,140,572 - +0.1 1,140,572 - +0.0 1,140,572 - -1.7 1,140,573 + -1.4 1,140,573 + +2.0 1,140,573 + +3.5 1,140,573 + -0.3 1,140,573 + +0.3 1,140,573 + -1.8 1,140,573 + +3.0 1,140,573 + +1.6 1,140,576 - +1.0 1,140,577 + -2.1 1,140,580 - -1.3 1,140,580 - -0.1 1,140,580 - +1.6 1,140,585 - +3.1 1,140,643 + -0.1 1,140,762 + MMJJ_RS06145 0.22 -0.9 1,140,940 + MMJJ_RS06145 0.48 -0.3 1,141,207 + MMJJ_RS06145 0.88 -0.7 1,141,399 - MMJJ_RS06150 0.18 -0.1 1,141,495 - MMJJ_RS06150 0.35 -2.1 1,141,495 - MMJJ_RS06150 0.35 +0.3 1,141,548 - MMJJ_RS06150 0.45 -1.2 1,141,555 - MMJJ_RS06150 0.46 -1.1
Or see this region's nucleotide sequence