Experiment: Formate N-free with 2mM Adenosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03650 and MMJJ_RS03655 are separated by 9 nucleotides MMJJ_RS03655 and MMJJ_RS03660 are separated by 0 nucleotides MMJJ_RS03660 and MMJJ_RS03665 are separated by 132 nucleotides
MMJJ_RS03650: MMJJ_RS03650 - class I SAM-dependent methyltransferase family protein, at 680,985 to 682,013
_RS03650
MMJJ_RS03655: MMJJ_RS03655 - replication factor C large subunit, at 682,023 to 683,483
_RS03655
MMJJ_RS03660: MMJJ_RS03660 - DUF447 family protein, at 683,484 to 684,029
_RS03660
MMJJ_RS03665: MMJJ_RS03665 - shikimate kinase, at 684,162 to 685,013
_RS03665
Position (kb) 682
683
684 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 681.055 kb on - strand at 681.056 kb on + strand at 681.059 kb on - strand at 681.062 kb on - strand at 681.099 kb on - strand, within MMJJ_RS03650 at 681.286 kb on + strand, within MMJJ_RS03650 at 681.476 kb on - strand, within MMJJ_RS03650 at 681.477 kb on + strand, within MMJJ_RS03650 at 681.483 kb on - strand, within MMJJ_RS03650 at 681.483 kb on - strand, within MMJJ_RS03650 at 681.484 kb on + strand, within MMJJ_RS03650 at 681.484 kb on + strand, within MMJJ_RS03650 at 681.528 kb on - strand, within MMJJ_RS03650 at 681.577 kb on - strand, within MMJJ_RS03650 at 681.578 kb on + strand, within MMJJ_RS03650 at 681.595 kb on - strand, within MMJJ_RS03650 at 681.595 kb on - strand, within MMJJ_RS03650 at 681.596 kb on + strand, within MMJJ_RS03650 at 681.629 kb on - strand, within MMJJ_RS03650 at 681.630 kb on + strand, within MMJJ_RS03650 at 681.712 kb on - strand, within MMJJ_RS03650 at 681.713 kb on + strand, within MMJJ_RS03650 at 681.788 kb on + strand, within MMJJ_RS03650 at 681.865 kb on + strand, within MMJJ_RS03650 at 681.868 kb on + strand, within MMJJ_RS03650 at 681.958 kb on + strand at 681.976 kb on - strand at 681.976 kb on - strand at 681.976 kb on - strand at 681.977 kb on + strand at 681.977 kb on + strand at 681.983 kb on + strand at 681.997 kb on - strand at 681.998 kb on + strand at 682.001 kb on + strand at 682.012 kb on + strand at 682.017 kb on + strand at 682.161 kb on + strand at 682.186 kb on + strand, within MMJJ_RS03655 at 682.186 kb on + strand, within MMJJ_RS03655 at 682.242 kb on + strand, within MMJJ_RS03655 at 682.260 kb on - strand, within MMJJ_RS03655 at 682.311 kb on + strand, within MMJJ_RS03655 at 682.512 kb on + strand, within MMJJ_RS03655 at 682.732 kb on + strand, within MMJJ_RS03655 at 683.067 kb on - strand at 683.297 kb on + strand at 683.485 kb on - strand at 683.494 kb on + strand at 683.608 kb on + strand, within MMJJ_RS03660 at 683.647 kb on - strand, within MMJJ_RS03660 at 683.712 kb on + strand, within MMJJ_RS03660 at 683.869 kb on - strand, within MMJJ_RS03660 at 683.882 kb on - strand, within MMJJ_RS03660 at 683.887 kb on + strand, within MMJJ_RS03660 at 683.893 kb on - strand, within MMJJ_RS03660 at 683.893 kb on - strand, within MMJJ_RS03660 at 683.894 kb on + strand at 683.938 kb on - strand, within MMJJ_RS03660 at 684.030 kb on + strand at 684.030 kb on + strand at 684.043 kb on + strand at 684.056 kb on - strand at 684.056 kb on - strand at 684.057 kb on + strand at 684.057 kb on + strand at 684.060 kb on + strand at 684.070 kb on + strand at 684.070 kb on + strand at 684.071 kb on - strand at 684.071 kb on - strand at 684.071 kb on - strand at 684.074 kb on - strand at 684.075 kb on + strand at 684.077 kb on - strand at 684.077 kb on - strand at 684.077 kb on - strand at 684.077 kb on - strand at 684.078 kb on + strand at 684.078 kb on + strand at 684.079 kb on - strand at 684.082 kb on - strand at 684.082 kb on - strand at 684.082 kb on - strand at 684.082 kb on - strand at 684.083 kb on + strand at 684.085 kb on - strand at 684.085 kb on - strand at 684.085 kb on - strand at 684.086 kb on + strand at 684.086 kb on + strand at 684.086 kb on + strand at 684.086 kb on + strand at 684.086 kb on + strand at 684.086 kb on + strand at 684.098 kb on - strand at 684.099 kb on + strand at 684.099 kb on + strand at 684.103 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate N-free with 2mM Adenosine remove 681,055 - -1.7 681,056 + -1.0 681,059 - -0.7 681,062 - -2.7 681,099 - MMJJ_RS03650 0.11 -2.3 681,286 + MMJJ_RS03650 0.29 -2.1 681,476 - MMJJ_RS03650 0.48 -0.9 681,477 + MMJJ_RS03650 0.48 -1.1 681,483 - MMJJ_RS03650 0.48 +0.0 681,483 - MMJJ_RS03650 0.48 -2.9 681,484 + MMJJ_RS03650 0.48 -0.6 681,484 + MMJJ_RS03650 0.48 -1.4 681,528 - MMJJ_RS03650 0.53 -3.2 681,577 - MMJJ_RS03650 0.58 -2.6 681,578 + MMJJ_RS03650 0.58 -2.9 681,595 - MMJJ_RS03650 0.59 -2.8 681,595 - MMJJ_RS03650 0.59 -2.0 681,596 + MMJJ_RS03650 0.59 -2.2 681,629 - MMJJ_RS03650 0.63 -1.2 681,630 + MMJJ_RS03650 0.63 -2.1 681,712 - MMJJ_RS03650 0.71 -0.2 681,713 + MMJJ_RS03650 0.71 -0.7 681,788 + MMJJ_RS03650 0.78 -1.1 681,865 + MMJJ_RS03650 0.86 -1.6 681,868 + MMJJ_RS03650 0.86 -2.0 681,958 + -2.4 681,976 - -1.6 681,976 - +0.6 681,976 - -1.6 681,977 + +0.0 681,977 + -0.8 681,983 + -1.2 681,997 - +1.7 681,998 + -3.9 682,001 + +3.7 682,012 + -3.4 682,017 + +1.1 682,161 + -3.0 682,186 + MMJJ_RS03655 0.11 -2.0 682,186 + MMJJ_RS03655 0.11 -3.5 682,242 + MMJJ_RS03655 0.15 -0.6 682,260 - MMJJ_RS03655 0.16 -3.9 682,311 + MMJJ_RS03655 0.20 -1.2 682,512 + MMJJ_RS03655 0.33 -1.6 682,732 + MMJJ_RS03655 0.49 -2.2 683,067 - +0.6 683,297 + +0.6 683,485 - -0.4 683,494 + -2.6 683,608 + MMJJ_RS03660 0.23 -1.2 683,647 - MMJJ_RS03660 0.30 -2.0 683,712 + MMJJ_RS03660 0.42 -2.2 683,869 - MMJJ_RS03660 0.71 -1.3 683,882 - MMJJ_RS03660 0.73 -1.2 683,887 + MMJJ_RS03660 0.74 -1.6 683,893 - MMJJ_RS03660 0.75 -1.7 683,893 - MMJJ_RS03660 0.75 -2.3 683,894 + -0.7 683,938 - MMJJ_RS03660 0.83 -1.7 684,030 + -1.0 684,030 + -1.0 684,043 + -2.8 684,056 - -2.5 684,056 - -0.7 684,057 + -1.7 684,057 + -0.3 684,060 + -0.6 684,070 + -1.3 684,070 + +1.0 684,071 - -1.3 684,071 - -0.3 684,071 - +1.2 684,074 - -2.5 684,075 + -1.4 684,077 - -0.6 684,077 - +0.0 684,077 - +1.5 684,077 - -1.2 684,078 + -1.1 684,078 + -2.1 684,079 - -0.6 684,082 - -1.3 684,082 - -2.0 684,082 - +1.8 684,082 - -2.2 684,083 + +0.6 684,085 - +1.7 684,085 - +1.6 684,085 - -2.2 684,086 + -3.2 684,086 + -0.5 684,086 + -1.2 684,086 + +0.0 684,086 + -1.1 684,086 + +0.2 684,098 - -1.0 684,099 + -0.4 684,099 + -0.4 684,103 - -1.6
Or see this region's nucleotide sequence