Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS00045

Experiment: Formate N-free with 2mM Adenosine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS00035 and MMJJ_RS00040 are separated by 9 nucleotidesMMJJ_RS00040 and MMJJ_RS00045 are separated by 11 nucleotidesMMJJ_RS00045 and MMJJ_RS00050 are separated by 10 nucleotidesMMJJ_RS00050 and MMJJ_RS00055 are separated by 14 nucleotides MMJJ_RS00035: MMJJ_RS00035 - CO dehydrogenase/acetyl-CoA synthase complex subunit alpha, at 6,409 to 8,751 _RS00035 MMJJ_RS00040: MMJJ_RS00040 - CO dehydrogenase/acetyl-CoA synthase complex subunit epsilon, at 8,761 to 9,279 _RS00040 MMJJ_RS00045: MMJJ_RS00045 - CO dehydrogenase/CO-methylating acetyl-CoA synthase complex subunit beta, at 9,291 to 10,664 _RS00045 MMJJ_RS00050: MMJJ_RS00050 - AAA family ATPase, at 10,675 to 11,427 _RS00050 MMJJ_RS00055: MMJJ_RS00055 - CO dehydrogenase/acetyl-CoA synthase subunit delta, at 11,442 to 12,596 _RS00055 Position (kb) 9 10 11Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 8.335 kb on + strand, within MMJJ_RS00035at 8.380 kb on - strand, within MMJJ_RS00035at 8.380 kb on - strand, within MMJJ_RS00035at 8.426 kb on - strand, within MMJJ_RS00035at 8.507 kb on - strand, within MMJJ_RS00035at 8.507 kb on - strand, within MMJJ_RS00035at 8.508 kb on + strand, within MMJJ_RS00035at 8.528 kb on - strandat 8.547 kb on - strandat 8.562 kb on + strandat 8.579 kb on - strandat 8.579 kb on - strandat 8.579 kb on - strandat 8.580 kb on + strandat 8.585 kb on - strandat 8.596 kb on - strandat 8.622 kb on + strandat 8.627 kb on - strandat 8.628 kb on + strandat 8.749 kb on - strandat 8.772 kb on - strandat 8.773 kb on + strandat 8.799 kb on + strandat 8.799 kb on + strandat 8.829 kb on + strand, within MMJJ_RS00040at 8.897 kb on - strand, within MMJJ_RS00040at 8.954 kb on - strand, within MMJJ_RS00040at 8.955 kb on + strand, within MMJJ_RS00040at 9.038 kb on - strand, within MMJJ_RS00040at 9.039 kb on + strand, within MMJJ_RS00040at 9.118 kb on - strand, within MMJJ_RS00040at 9.118 kb on - strand, within MMJJ_RS00040at 9.118 kb on - strand, within MMJJ_RS00040at 9.119 kb on + strand, within MMJJ_RS00040at 9.119 kb on + strand, within MMJJ_RS00040at 9.121 kb on - strand, within MMJJ_RS00040at 9.122 kb on + strand, within MMJJ_RS00040at 9.122 kb on + strand, within MMJJ_RS00040at 9.122 kb on + strand, within MMJJ_RS00040at 9.194 kb on + strand, within MMJJ_RS00040at 9.194 kb on + strand, within MMJJ_RS00040at 9.228 kb on - strandat 9.228 kb on - strandat 9.229 kb on + strandat 9.229 kb on + strandat 9.229 kb on + strandat 9.229 kb on + strandat 9.229 kb on + strandat 9.253 kb on - strandat 9.304 kb on - strandat 9.305 kb on + strandat 9.316 kb on - strandat 9.317 kb on + strandat 9.327 kb on - strandat 9.327 kb on - strandat 9.328 kb on + strandat 9.407 kb on + strandat 9.422 kb on + strandat 9.545 kb on + strand, within MMJJ_RS00045at 9.576 kb on - strand, within MMJJ_RS00045at 9.576 kb on - strand, within MMJJ_RS00045at 9.576 kb on - strand, within MMJJ_RS00045at 9.591 kb on + strand, within MMJJ_RS00045at 9.598 kb on - strand, within MMJJ_RS00045at 9.599 kb on + strand, within MMJJ_RS00045at 9.599 kb on + strand, within MMJJ_RS00045at 9.661 kb on - strand, within MMJJ_RS00045at 9.736 kb on - strand, within MMJJ_RS00045at 9.736 kb on - strand, within MMJJ_RS00045at 9.757 kb on - strand, within MMJJ_RS00045at 9.807 kb on + strand, within MMJJ_RS00045at 9.842 kb on + strand, within MMJJ_RS00045at 9.846 kb on - strand, within MMJJ_RS00045at 9.847 kb on + strand, within MMJJ_RS00045at 9.855 kb on + strand, within MMJJ_RS00045at 9.892 kb on - strand, within MMJJ_RS00045at 9.944 kb on - strand, within MMJJ_RS00045at 10.027 kb on + strand, within MMJJ_RS00045at 10.031 kb on - strand, within MMJJ_RS00045at 10.031 kb on - strand, within MMJJ_RS00045at 10.032 kb on + strand, within MMJJ_RS00045at 10.055 kb on - strand, within MMJJ_RS00045at 10.067 kb on - strand, within MMJJ_RS00045at 10.068 kb on + strand, within MMJJ_RS00045at 10.082 kb on + strand, within MMJJ_RS00045at 10.084 kb on + strand, within MMJJ_RS00045at 10.087 kb on - strand, within MMJJ_RS00045at 10.088 kb on + strand, within MMJJ_RS00045at 10.138 kb on - strand, within MMJJ_RS00045at 10.146 kb on - strand, within MMJJ_RS00045at 10.199 kb on - strand, within MMJJ_RS00045at 10.200 kb on + strand, within MMJJ_RS00045at 10.281 kb on + strand, within MMJJ_RS00045at 10.281 kb on + strand, within MMJJ_RS00045at 10.287 kb on - strand, within MMJJ_RS00045at 10.287 kb on - strand, within MMJJ_RS00045at 10.288 kb on + strand, within MMJJ_RS00045at 10.288 kb on + strand, within MMJJ_RS00045at 10.288 kb on + strand, within MMJJ_RS00045at 10.289 kb on - strand, within MMJJ_RS00045at 10.290 kb on + strand, within MMJJ_RS00045at 10.358 kb on + strand, within MMJJ_RS00045at 10.365 kb on + strand, within MMJJ_RS00045at 10.393 kb on - strand, within MMJJ_RS00045at 10.393 kb on - strand, within MMJJ_RS00045at 10.393 kb on - strand, within MMJJ_RS00045at 10.611 kb on + strandat 10.612 kb on - strandat 10.613 kb on + strandat 10.652 kb on + strandat 10.652 kb on + strandat 10.731 kb on + strandat 10.775 kb on - strand, within MMJJ_RS00050at 10.775 kb on - strand, within MMJJ_RS00050at 10.776 kb on + strand, within MMJJ_RS00050at 10.869 kb on - strand, within MMJJ_RS00050at 10.882 kb on + strand, within MMJJ_RS00050at 10.882 kb on + strand, within MMJJ_RS00050at 10.914 kb on + strand, within MMJJ_RS00050at 10.914 kb on + strand, within MMJJ_RS00050at 10.940 kb on - strand, within MMJJ_RS00050at 10.997 kb on + strand, within MMJJ_RS00050at 11.050 kb on - strand, within MMJJ_RS00050at 11.060 kb on - strand, within MMJJ_RS00050at 11.061 kb on + strand, within MMJJ_RS00050at 11.126 kb on - strand, within MMJJ_RS00050at 11.127 kb on + strand, within MMJJ_RS00050at 11.127 kb on + strand, within MMJJ_RS00050at 11.133 kb on + strand, within MMJJ_RS00050at 11.228 kb on - strand, within MMJJ_RS00050at 11.235 kb on - strand, within MMJJ_RS00050at 11.235 kb on - strand, within MMJJ_RS00050at 11.299 kb on + strand, within MMJJ_RS00050at 11.310 kb on + strand, within MMJJ_RS00050at 11.335 kb on - strand, within MMJJ_RS00050at 11.497 kb on - strandat 11.518 kb on - strandat 11.519 kb on + strandat 11.533 kb on - strandat 11.540 kb on + strandat 11.540 kb on + strandat 11.546 kb on - strandat 11.552 kb on + strandat 11.629 kb on + strand, within MMJJ_RS00055

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate N-free with 2mM Adenosine
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8,335 + MMJJ_RS00035 0.82 -3.0
8,380 - MMJJ_RS00035 0.84 -2.3
8,380 - MMJJ_RS00035 0.84 -2.0
8,426 - MMJJ_RS00035 0.86 -1.5
8,507 - MMJJ_RS00035 0.90 -3.9
8,507 - MMJJ_RS00035 0.90 -2.4
8,508 + MMJJ_RS00035 0.90 -3.2
8,528 - -1.0
8,547 - -1.5
8,562 + -3.5
8,579 - -3.7
8,579 - -2.3
8,579 - -3.4
8,580 + -3.5
8,585 - -3.2
8,596 - -2.6
8,622 + -3.4
8,627 - -1.6
8,628 + -4.1
8,749 - -2.0
8,772 - -0.1
8,773 + -1.2
8,799 + -2.8
8,799 + -2.0
8,829 + MMJJ_RS00040 0.13 -3.8
8,897 - MMJJ_RS00040 0.26 -3.1
8,954 - MMJJ_RS00040 0.37 -2.5
8,955 + MMJJ_RS00040 0.37 -3.2
9,038 - MMJJ_RS00040 0.53 -2.9
9,039 + MMJJ_RS00040 0.54 -1.5
9,118 - MMJJ_RS00040 0.69 -1.0
9,118 - MMJJ_RS00040 0.69 -2.9
9,118 - MMJJ_RS00040 0.69 -4.1
9,119 + MMJJ_RS00040 0.69 -3.0
9,119 + MMJJ_RS00040 0.69 -1.1
9,121 - MMJJ_RS00040 0.69 -2.7
9,122 + MMJJ_RS00040 0.70 +0.1
9,122 + MMJJ_RS00040 0.70 -0.7
9,122 + MMJJ_RS00040 0.70 -2.5
9,194 + MMJJ_RS00040 0.83 -4.6
9,194 + MMJJ_RS00040 0.83 -1.7
9,228 - -3.8
9,228 - -1.8
9,229 + -1.1
9,229 + -2.3
9,229 + -3.6
9,229 + -4.6
9,229 + -2.5
9,253 - -4.0
9,304 - -2.0
9,305 + -3.5
9,316 - -3.7
9,317 + -2.3
9,327 - -2.3
9,327 - -2.0
9,328 + -2.9
9,407 + -3.4
9,422 + -3.5
9,545 + MMJJ_RS00045 0.18 -4.0
9,576 - MMJJ_RS00045 0.21 -1.3
9,576 - MMJJ_RS00045 0.21 -2.0
9,576 - MMJJ_RS00045 0.21 -3.7
9,591 + MMJJ_RS00045 0.22 -2.7
9,598 - MMJJ_RS00045 0.22 -1.1
9,599 + MMJJ_RS00045 0.22 -3.7
9,599 + MMJJ_RS00045 0.22 -3.6
9,661 - MMJJ_RS00045 0.27 -2.5
9,736 - MMJJ_RS00045 0.32 -1.0
9,736 - MMJJ_RS00045 0.32 -3.4
9,757 - MMJJ_RS00045 0.34 -2.4
9,807 + MMJJ_RS00045 0.38 -0.3
9,842 + MMJJ_RS00045 0.40 -2.5
9,846 - MMJJ_RS00045 0.40 -1.5
9,847 + MMJJ_RS00045 0.40 +0.1
9,855 + MMJJ_RS00045 0.41 -3.1
9,892 - MMJJ_RS00045 0.44 -3.1
9,944 - MMJJ_RS00045 0.48 -2.4
10,027 + MMJJ_RS00045 0.54 -2.5
10,031 - MMJJ_RS00045 0.54 -3.0
10,031 - MMJJ_RS00045 0.54 -3.5
10,032 + MMJJ_RS00045 0.54 -3.6
10,055 - MMJJ_RS00045 0.56 -2.7
10,067 - MMJJ_RS00045 0.56 -3.1
10,068 + MMJJ_RS00045 0.57 -3.0
10,082 + MMJJ_RS00045 0.58 -2.1
10,084 + MMJJ_RS00045 0.58 -1.2
10,087 - MMJJ_RS00045 0.58 -3.6
10,088 + MMJJ_RS00045 0.58 -2.7
10,138 - MMJJ_RS00045 0.62 -2.7
10,146 - MMJJ_RS00045 0.62 -4.6
10,199 - MMJJ_RS00045 0.66 -4.0
10,200 + MMJJ_RS00045 0.66 -1.7
10,281 + MMJJ_RS00045 0.72 -1.1
10,281 + MMJJ_RS00045 0.72 -1.4
10,287 - MMJJ_RS00045 0.72 -3.7
10,287 - MMJJ_RS00045 0.72 -2.0
10,288 + MMJJ_RS00045 0.73 -3.6
10,288 + MMJJ_RS00045 0.73 -3.0
10,288 + MMJJ_RS00045 0.73 -2.5
10,289 - MMJJ_RS00045 0.73 -3.5
10,290 + MMJJ_RS00045 0.73 -1.8
10,358 + MMJJ_RS00045 0.78 -1.6
10,365 + MMJJ_RS00045 0.78 -2.8
10,393 - MMJJ_RS00045 0.80 -4.3
10,393 - MMJJ_RS00045 0.80 -3.5
10,393 - MMJJ_RS00045 0.80 -2.2
10,611 + -1.2
10,612 - +1.7
10,613 + -1.9
10,652 + -2.4
10,652 + -2.2
10,731 + -3.1
10,775 - MMJJ_RS00050 0.13 -3.0
10,775 - MMJJ_RS00050 0.13 -2.0
10,776 + MMJJ_RS00050 0.13 -3.1
10,869 - MMJJ_RS00050 0.26 -1.5
10,882 + MMJJ_RS00050 0.27 -3.0
10,882 + MMJJ_RS00050 0.27 -1.5
10,914 + MMJJ_RS00050 0.32 -2.5
10,914 + MMJJ_RS00050 0.32 -1.2
10,940 - MMJJ_RS00050 0.35 -3.1
10,997 + MMJJ_RS00050 0.43 -4.4
11,050 - MMJJ_RS00050 0.50 -3.8
11,060 - MMJJ_RS00050 0.51 -2.0
11,061 + MMJJ_RS00050 0.51 -2.2
11,126 - MMJJ_RS00050 0.60 -3.8
11,127 + MMJJ_RS00050 0.60 -3.9
11,127 + MMJJ_RS00050 0.60 -1.6
11,133 + MMJJ_RS00050 0.61 -2.9
11,228 - MMJJ_RS00050 0.73 -1.0
11,235 - MMJJ_RS00050 0.74 -1.5
11,235 - MMJJ_RS00050 0.74 -2.4
11,299 + MMJJ_RS00050 0.83 -3.7
11,310 + MMJJ_RS00050 0.84 -4.0
11,335 - MMJJ_RS00050 0.88 -3.0
11,497 - -3.0
11,518 - -1.5
11,519 + -3.4
11,533 - -2.1
11,540 + -1.2
11,540 + -1.3
11,546 - -1.8
11,552 + -3.3
11,629 + MMJJ_RS00055 0.16 -3.5

Or see this region's nucleotide sequence