Experiment: Polymyxin B sulfate 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ga0059261_3942 and Ga0059261_3943 overlap by 4 nucleotides Ga0059261_3943 and Ga0059261_3944 overlap by 10 nucleotides Ga0059261_3944 and Ga0059261_3945 overlap by 7 nucleotides Ga0059261_3945 and Ga0059261_3946 are separated by 11 nucleotides Ga0059261_3946 and Ga0059261_3947 overlap by 4 nucleotides
Ga0059261_3942: Ga0059261_3942 - hypothetical protein, at 4,059,217 to 4,059,729
_3942
Ga0059261_3943: Ga0059261_3943 - Type II secretion system (T2SS), protein M subtype b, at 4,059,726 to 4,060,193
_3943
Ga0059261_3944: Ga0059261_3944 - hypothetical protein, at 4,060,184 to 4,060,636
_3944
Ga0059261_3945: Ga0059261_3945 - type II secretion system protein D, at 4,060,630 to 4,062,690
_3945
Ga0059261_3946: Ga0059261_3946 - Type IV pilus biogenesis/PDZ domain, at 4,062,702 to 4,063,469
_3946
Ga0059261_3947: Ga0059261_3947 - type II secretion system protein I (GspI), at 4,063,466 to 4,063,945
_3947
Position (kb)
4060
4061
4062
4063 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4059.958 kb on + strand, within Ga0059261_3943 at 4060.260 kb on + strand, within Ga0059261_3944 at 4060.260 kb on + strand, within Ga0059261_3944 at 4060.260 kb on + strand, within Ga0059261_3944 at 4060.260 kb on + strand, within Ga0059261_3944 at 4060.349 kb on + strand, within Ga0059261_3944 at 4060.349 kb on + strand, within Ga0059261_3944 at 4060.349 kb on + strand, within Ga0059261_3944 at 4060.349 kb on + strand, within Ga0059261_3944 at 4060.349 kb on + strand, within Ga0059261_3944 at 4060.350 kb on - strand, within Ga0059261_3944 at 4060.350 kb on - strand, within Ga0059261_3944 at 4060.733 kb on + strand at 4060.733 kb on + strand at 4060.733 kb on + strand at 4060.733 kb on + strand at 4060.733 kb on + strand at 4060.734 kb on - strand at 4060.734 kb on - strand at 4060.734 kb on - strand at 4060.734 kb on - strand at 4060.806 kb on + strand at 4060.806 kb on + strand at 4060.806 kb on + strand at 4060.807 kb on - strand at 4060.898 kb on + strand, within Ga0059261_3945 at 4060.899 kb on - strand, within Ga0059261_3945 at 4060.899 kb on - strand at 4061.123 kb on + strand, within Ga0059261_3945 at 4061.123 kb on + strand, within Ga0059261_3945 at 4061.123 kb on + strand, within Ga0059261_3945 at 4061.308 kb on - strand, within Ga0059261_3945 at 4061.786 kb on + strand, within Ga0059261_3945 at 4061.786 kb on + strand, within Ga0059261_3945 at 4061.786 kb on + strand, within Ga0059261_3945 at 4061.787 kb on - strand, within Ga0059261_3945 at 4061.787 kb on - strand, within Ga0059261_3945 at 4061.891 kb on + strand, within Ga0059261_3945 at 4061.891 kb on + strand, within Ga0059261_3945 at 4061.891 kb on + strand, within Ga0059261_3945 at 4061.892 kb on - strand, within Ga0059261_3945 at 4061.892 kb on - strand, within Ga0059261_3945 at 4061.892 kb on - strand, within Ga0059261_3945 at 4062.188 kb on + strand, within Ga0059261_3945 at 4062.188 kb on + strand, within Ga0059261_3945 at 4062.188 kb on + strand, within Ga0059261_3945 at 4062.189 kb on - strand, within Ga0059261_3945 at 4062.189 kb on - strand, within Ga0059261_3945 at 4062.189 kb on - strand, within Ga0059261_3945 at 4062.416 kb on + strand, within Ga0059261_3945 at 4062.416 kb on + strand, within Ga0059261_3945 at 4062.416 kb on + strand, within Ga0059261_3945 at 4062.416 kb on + strand, within Ga0059261_3945 at 4062.417 kb on - strand, within Ga0059261_3945 at 4062.417 kb on - strand, within Ga0059261_3945 at 4062.417 kb on - strand, within Ga0059261_3945 at 4062.537 kb on + strand at 4062.699 kb on + strand at 4062.700 kb on - strand at 4062.703 kb on + strand at 4062.704 kb on - strand at 4063.039 kb on + strand, within Ga0059261_3946 at 4063.039 kb on + strand, within Ga0059261_3946 at 4063.039 kb on + strand, within Ga0059261_3946 at 4063.040 kb on - strand, within Ga0059261_3946 at 4063.040 kb on - strand, within Ga0059261_3946 at 4063.157 kb on + strand, within Ga0059261_3946 at 4063.159 kb on + strand, within Ga0059261_3946 at 4063.159 kb on + strand, within Ga0059261_3946 at 4063.159 kb on + strand, within Ga0059261_3946 at 4063.159 kb on + strand, within Ga0059261_3946 at 4063.160 kb on - strand, within Ga0059261_3946 at 4063.160 kb on - strand, within Ga0059261_3946 at 4063.160 kb on - strand, within Ga0059261_3946 at 4063.160 kb on - strand, within Ga0059261_3946 at 4063.160 kb on - strand, within Ga0059261_3946 at 4063.471 kb on + strand at 4063.471 kb on + strand at 4063.471 kb on + strand at 4063.471 kb on + strand at 4063.471 kb on + strand at 4063.471 kb on + strand at 4063.471 kb on + strand at 4063.471 kb on + strand at 4063.471 kb on + strand at 4063.472 kb on - strand at 4063.472 kb on - strand at 4063.472 kb on - strand at 4063.472 kb on - strand at 4063.472 kb on - strand at 4063.472 kb on - strand at 4063.472 kb on - strand at 4063.472 kb on - strand at 4063.472 kb on - strand at 4063.472 kb on - strand at 4063.472 kb on - strand at 4063.532 kb on + strand, within Ga0059261_3947 at 4063.533 kb on - strand, within Ga0059261_3947
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.05 mg/ml remove 4,059,958 + Ga0059261_3943 0.50 +1.1 4,060,260 + Ga0059261_3944 0.17 +0.4 4,060,260 + Ga0059261_3944 0.17 -0.8 4,060,260 + Ga0059261_3944 0.17 +0.3 4,060,260 + Ga0059261_3944 0.17 +0.1 4,060,349 + Ga0059261_3944 0.36 +0.3 4,060,349 + Ga0059261_3944 0.36 -0.0 4,060,349 + Ga0059261_3944 0.36 -0.1 4,060,349 + Ga0059261_3944 0.36 +0.1 4,060,349 + Ga0059261_3944 0.36 +0.3 4,060,350 - Ga0059261_3944 0.37 -0.7 4,060,350 - Ga0059261_3944 0.37 -0.7 4,060,733 + -0.9 4,060,733 + -0.1 4,060,733 + +0.0 4,060,733 + -0.3 4,060,733 + -1.6 4,060,734 - -0.5 4,060,734 - -0.9 4,060,734 - +0.9 4,060,734 - -2.1 4,060,806 + -1.3 4,060,806 + +0.1 4,060,806 + -0.1 4,060,807 - -1.1 4,060,898 + Ga0059261_3945 0.13 +0.3 4,060,899 - Ga0059261_3945 0.13 +0.9 4,060,899 - +0.9 4,061,123 + Ga0059261_3945 0.24 -0.6 4,061,123 + Ga0059261_3945 0.24 -0.1 4,061,123 + Ga0059261_3945 0.24 -1.4 4,061,308 - Ga0059261_3945 0.33 -1.6 4,061,786 + Ga0059261_3945 0.56 -0.4 4,061,786 + Ga0059261_3945 0.56 -1.1 4,061,786 + Ga0059261_3945 0.56 -0.3 4,061,787 - Ga0059261_3945 0.56 +0.6 4,061,787 - Ga0059261_3945 0.56 -0.5 4,061,891 + Ga0059261_3945 0.61 +0.1 4,061,891 + Ga0059261_3945 0.61 -0.1 4,061,891 + Ga0059261_3945 0.61 -0.2 4,061,892 - Ga0059261_3945 0.61 -1.0 4,061,892 - Ga0059261_3945 0.61 -1.0 4,061,892 - Ga0059261_3945 0.61 -0.6 4,062,188 + Ga0059261_3945 0.76 -0.0 4,062,188 + Ga0059261_3945 0.76 -0.8 4,062,188 + Ga0059261_3945 0.76 -1.1 4,062,189 - Ga0059261_3945 0.76 -0.3 4,062,189 - Ga0059261_3945 0.76 -0.9 4,062,189 - Ga0059261_3945 0.76 -0.5 4,062,416 + Ga0059261_3945 0.87 +0.3 4,062,416 + Ga0059261_3945 0.87 -0.3 4,062,416 + Ga0059261_3945 0.87 -0.6 4,062,416 + Ga0059261_3945 0.87 -0.7 4,062,417 - Ga0059261_3945 0.87 -0.4 4,062,417 - Ga0059261_3945 0.87 -1.3 4,062,417 - Ga0059261_3945 0.87 -1.1 4,062,537 + +0.3 4,062,699 + +0.5 4,062,700 - -0.2 4,062,703 + -1.1 4,062,704 - -0.5 4,063,039 + Ga0059261_3946 0.44 +0.1 4,063,039 + Ga0059261_3946 0.44 -0.8 4,063,039 + Ga0059261_3946 0.44 -1.1 4,063,040 - Ga0059261_3946 0.44 -0.3 4,063,040 - Ga0059261_3946 0.44 -0.2 4,063,157 + Ga0059261_3946 0.59 -0.0 4,063,159 + Ga0059261_3946 0.60 -1.2 4,063,159 + Ga0059261_3946 0.60 +0.6 4,063,159 + Ga0059261_3946 0.60 -0.7 4,063,159 + Ga0059261_3946 0.60 +1.9 4,063,160 - Ga0059261_3946 0.60 -0.7 4,063,160 - Ga0059261_3946 0.60 -0.5 4,063,160 - Ga0059261_3946 0.60 -0.3 4,063,160 - Ga0059261_3946 0.60 +0.1 4,063,160 - Ga0059261_3946 0.60 -0.1 4,063,471 + +0.2 4,063,471 + -0.7 4,063,471 + -0.7 4,063,471 + -0.5 4,063,471 + -0.1 4,063,471 + -0.0 4,063,471 + +1.7 4,063,471 + +1.9 4,063,471 + -0.4 4,063,472 - -0.9 4,063,472 - -0.1 4,063,472 - -0.9 4,063,472 - -0.8 4,063,472 - -1.1 4,063,472 - +1.3 4,063,472 - -0.0 4,063,472 - -0.5 4,063,472 - +0.9 4,063,472 - -0.2 4,063,472 - -1.1 4,063,532 + Ga0059261_3947 0.14 +1.0 4,063,533 - Ga0059261_3947 0.14 +0.6
Or see this region's nucleotide sequence