Experiment: Recovery broth with Isovaleric 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS11180 and BBR_RS11185 are separated by 103 nucleotides BBR_RS11185 and BBR_RS11190 are separated by 318 nucleotides BBR_RS11190 and BBR_RS11195 overlap by 4 nucleotides
BBR_RS11180: BBR_RS11180 - phosphatase PAP2 family protein, at 251,394 to 252,572
_RS11180
BBR_RS11185: BBR_RS11185 - type I DNA topoisomerase, at 252,676 to 255,726
_RS11185
BBR_RS11190: BBR_RS11190 - dTMP kinase, at 256,045 to 256,665
_RS11190
BBR_RS11195: BBR_RS11195 - DNA polymerase III subunit delta', at 256,662 to 257,813
_RS11195
Position (kb) 252
253
254
255
256 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 251.689 kb on + strand, within BBR_RS11180 at 251.689 kb on + strand, within BBR_RS11180 at 251.691 kb on + strand, within BBR_RS11180 at 251.692 kb on - strand, within BBR_RS11180 at 251.697 kb on - strand, within BBR_RS11180 at 251.728 kb on - strand, within BBR_RS11180 at 251.743 kb on + strand, within BBR_RS11180 at 251.751 kb on - strand, within BBR_RS11180 at 251.751 kb on - strand, within BBR_RS11180 at 251.783 kb on + strand, within BBR_RS11180 at 251.805 kb on - strand, within BBR_RS11180 at 251.844 kb on - strand, within BBR_RS11180 at 251.856 kb on + strand, within BBR_RS11180 at 251.874 kb on + strand, within BBR_RS11180 at 251.874 kb on + strand, within BBR_RS11180 at 251.919 kb on + strand, within BBR_RS11180 at 251.938 kb on + strand, within BBR_RS11180 at 251.943 kb on - strand, within BBR_RS11180 at 251.943 kb on - strand, within BBR_RS11180 at 251.969 kb on - strand, within BBR_RS11180 at 251.969 kb on - strand, within BBR_RS11180 at 251.974 kb on - strand, within BBR_RS11180 at 251.987 kb on + strand, within BBR_RS11180 at 251.987 kb on + strand, within BBR_RS11180 at 251.987 kb on + strand, within BBR_RS11180 at 251.995 kb on - strand, within BBR_RS11180 at 251.995 kb on - strand, within BBR_RS11180 at 251.995 kb on - strand, within BBR_RS11180 at 251.995 kb on - strand, within BBR_RS11180 at 251.995 kb on - strand, within BBR_RS11180 at 251.995 kb on - strand, within BBR_RS11180 at 251.997 kb on + strand, within BBR_RS11180 at 252.017 kb on + strand, within BBR_RS11180 at 252.025 kb on - strand, within BBR_RS11180 at 252.025 kb on - strand, within BBR_RS11180 at 252.025 kb on - strand, within BBR_RS11180 at 252.039 kb on + strand, within BBR_RS11180 at 252.057 kb on + strand, within BBR_RS11180 at 252.057 kb on + strand, within BBR_RS11180 at 252.066 kb on + strand, within BBR_RS11180 at 252.070 kb on - strand, within BBR_RS11180 at 252.074 kb on - strand, within BBR_RS11180 at 252.082 kb on + strand, within BBR_RS11180 at 252.107 kb on + strand, within BBR_RS11180 at 252.109 kb on + strand, within BBR_RS11180 at 252.109 kb on + strand, within BBR_RS11180 at 252.152 kb on + strand, within BBR_RS11180 at 252.160 kb on - strand, within BBR_RS11180 at 252.160 kb on - strand, within BBR_RS11180 at 252.160 kb on - strand, within BBR_RS11180 at 252.205 kb on - strand, within BBR_RS11180 at 252.212 kb on + strand, within BBR_RS11180 at 252.214 kb on + strand, within BBR_RS11180 at 252.222 kb on - strand, within BBR_RS11180 at 252.222 kb on - strand, within BBR_RS11180 at 252.222 kb on - strand, within BBR_RS11180 at 252.222 kb on - strand, within BBR_RS11180 at 252.224 kb on + strand, within BBR_RS11180 at 252.234 kb on + strand, within BBR_RS11180 at 252.235 kb on + strand, within BBR_RS11180 at 252.235 kb on + strand, within BBR_RS11180 at 252.243 kb on - strand, within BBR_RS11180 at 252.255 kb on + strand, within BBR_RS11180 at 252.273 kb on + strand, within BBR_RS11180 at 252.274 kb on + strand, within BBR_RS11180 at 252.280 kb on - strand, within BBR_RS11180 at 252.286 kb on - strand, within BBR_RS11180 at 252.287 kb on - strand, within BBR_RS11180 at 252.292 kb on + strand, within BBR_RS11180 at 252.296 kb on + strand, within BBR_RS11180 at 252.298 kb on + strand, within BBR_RS11180 at 252.304 kb on - strand, within BBR_RS11180 at 252.330 kb on + strand, within BBR_RS11180 at 252.330 kb on + strand, within BBR_RS11180 at 252.336 kb on - strand, within BBR_RS11180 at 252.346 kb on + strand, within BBR_RS11180 at 252.356 kb on - strand, within BBR_RS11180 at 252.358 kb on - strand, within BBR_RS11180 at 252.375 kb on - strand, within BBR_RS11180 at 252.375 kb on - strand, within BBR_RS11180 at 252.384 kb on - strand, within BBR_RS11180 at 252.431 kb on - strand, within BBR_RS11180 at 252.432 kb on + strand, within BBR_RS11180 at 252.433 kb on - strand, within BBR_RS11180 at 252.433 kb on - strand, within BBR_RS11180 at 252.447 kb on + strand, within BBR_RS11180 at 252.457 kb on + strand at 252.478 kb on + strand at 252.478 kb on + strand at 252.478 kb on + strand at 252.486 kb on - strand at 252.486 kb on - strand at 252.486 kb on - strand at 252.486 kb on - strand at 252.488 kb on + strand at 252.503 kb on + strand at 252.511 kb on - strand at 252.524 kb on + strand at 252.524 kb on + strand at 252.527 kb on - strand at 252.527 kb on - strand at 252.534 kb on + strand at 252.534 kb on + strand at 252.557 kb on - strand at 252.558 kb on + strand at 252.571 kb on + strand at 252.602 kb on - strand at 252.623 kb on - strand at 252.623 kb on - strand at 252.660 kb on + strand at 252.667 kb on + strand at 252.667 kb on + strand at 252.667 kb on + strand at 254.714 kb on - strand, within BBR_RS11185 at 255.225 kb on + strand, within BBR_RS11185 at 255.233 kb on - strand, within BBR_RS11185 at 255.265 kb on - strand, within BBR_RS11185 at 255.306 kb on + strand, within BBR_RS11185 at 255.371 kb on + strand, within BBR_RS11185 at 255.455 kb on + strand at 255.495 kb on + strand at 255.495 kb on + strand at 255.503 kb on - strand at 255.511 kb on + strand at 255.537 kb on + strand at 255.540 kb on - strand at 255.572 kb on + strand at 255.572 kb on + strand at 255.605 kb on + strand at 255.605 kb on + strand at 255.605 kb on + strand at 255.613 kb on - strand at 255.613 kb on - strand at 255.615 kb on + strand at 255.638 kb on - strand at 255.640 kb on + strand at 255.661 kb on + strand at 255.671 kb on + strand at 255.697 kb on - strand at 255.704 kb on - strand at 255.794 kb on + strand at 255.799 kb on + strand at 255.799 kb on + strand at 255.810 kb on + strand at 255.812 kb on + strand at 255.823 kb on + strand at 255.823 kb on + strand at 255.831 kb on - strand at 255.832 kb on - strand at 255.838 kb on + strand at 255.865 kb on - strand at 255.867 kb on + strand at 255.867 kb on + strand at 255.875 kb on - strand at 255.899 kb on + strand at 255.899 kb on + strand at 255.918 kb on + strand at 255.923 kb on + strand at 255.926 kb on - strand at 255.926 kb on - strand at 255.982 kb on + strand at 255.991 kb on - strand at 256.011 kb on + strand at 256.011 kb on + strand at 256.017 kb on - strand at 256.028 kb on + strand at 256.029 kb on + strand at 256.037 kb on - strand at 256.659 kb on + strand at 256.673 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 100 mM remove 251,689 + BBR_RS11180 0.25 +0.9 251,689 + BBR_RS11180 0.25 -1.6 251,691 + BBR_RS11180 0.25 -0.6 251,692 - BBR_RS11180 0.25 +0.1 251,697 - BBR_RS11180 0.26 -1.0 251,728 - BBR_RS11180 0.28 +0.4 251,743 + BBR_RS11180 0.30 -0.1 251,751 - BBR_RS11180 0.30 +0.3 251,751 - BBR_RS11180 0.30 -0.8 251,783 + BBR_RS11180 0.33 -0.3 251,805 - BBR_RS11180 0.35 -2.2 251,844 - BBR_RS11180 0.38 -1.4 251,856 + BBR_RS11180 0.39 -0.3 251,874 + BBR_RS11180 0.41 -1.5 251,874 + BBR_RS11180 0.41 -0.0 251,919 + BBR_RS11180 0.45 -0.3 251,938 + BBR_RS11180 0.46 -0.7 251,943 - BBR_RS11180 0.47 -1.2 251,943 - BBR_RS11180 0.47 -0.5 251,969 - BBR_RS11180 0.49 -1.0 251,969 - BBR_RS11180 0.49 -0.6 251,974 - BBR_RS11180 0.49 -1.0 251,987 + BBR_RS11180 0.50 -0.8 251,987 + BBR_RS11180 0.50 -0.3 251,987 + BBR_RS11180 0.50 -1.8 251,995 - BBR_RS11180 0.51 +0.8 251,995 - BBR_RS11180 0.51 -1.1 251,995 - BBR_RS11180 0.51 -0.4 251,995 - BBR_RS11180 0.51 -0.5 251,995 - BBR_RS11180 0.51 -0.3 251,995 - BBR_RS11180 0.51 -2.2 251,997 + BBR_RS11180 0.51 +0.0 252,017 + BBR_RS11180 0.53 -1.2 252,025 - BBR_RS11180 0.54 +0.2 252,025 - BBR_RS11180 0.54 -0.7 252,025 - BBR_RS11180 0.54 -0.3 252,039 + BBR_RS11180 0.55 -0.3 252,057 + BBR_RS11180 0.56 +0.8 252,057 + BBR_RS11180 0.56 -0.8 252,066 + BBR_RS11180 0.57 +0.5 252,070 - BBR_RS11180 0.57 -1.7 252,074 - BBR_RS11180 0.58 +0.1 252,082 + BBR_RS11180 0.58 -1.4 252,107 + BBR_RS11180 0.60 +0.1 252,109 + BBR_RS11180 0.61 -1.2 252,109 + BBR_RS11180 0.61 -1.4 252,152 + BBR_RS11180 0.64 -0.4 252,160 - BBR_RS11180 0.65 +0.5 252,160 - BBR_RS11180 0.65 -1.0 252,160 - BBR_RS11180 0.65 -2.6 252,205 - BBR_RS11180 0.69 -1.7 252,212 + BBR_RS11180 0.69 +0.9 252,214 + BBR_RS11180 0.70 -0.8 252,222 - BBR_RS11180 0.70 -1.6 252,222 - BBR_RS11180 0.70 +0.3 252,222 - BBR_RS11180 0.70 -0.6 252,222 - BBR_RS11180 0.70 -0.7 252,224 + BBR_RS11180 0.70 +0.5 252,234 + BBR_RS11180 0.71 -0.2 252,235 + BBR_RS11180 0.71 -2.0 252,235 + BBR_RS11180 0.71 +0.0 252,243 - BBR_RS11180 0.72 -0.9 252,255 + BBR_RS11180 0.73 -0.5 252,273 + BBR_RS11180 0.75 -0.5 252,274 + BBR_RS11180 0.75 -2.1 252,280 - BBR_RS11180 0.75 -0.6 252,286 - BBR_RS11180 0.76 -0.2 252,287 - BBR_RS11180 0.76 -0.6 252,292 + BBR_RS11180 0.76 -0.9 252,296 + BBR_RS11180 0.77 -0.0 252,298 + BBR_RS11180 0.77 -1.3 252,304 - BBR_RS11180 0.77 -1.1 252,330 + BBR_RS11180 0.79 -2.1 252,330 + BBR_RS11180 0.79 -0.6 252,336 - BBR_RS11180 0.80 -0.1 252,346 + BBR_RS11180 0.81 -0.7 252,356 - BBR_RS11180 0.82 +0.3 252,358 - BBR_RS11180 0.82 +0.2 252,375 - BBR_RS11180 0.83 -0.6 252,375 - BBR_RS11180 0.83 -2.4 252,384 - BBR_RS11180 0.84 -0.1 252,431 - BBR_RS11180 0.88 -1.0 252,432 + BBR_RS11180 0.88 -0.5 252,433 - BBR_RS11180 0.88 -2.1 252,433 - BBR_RS11180 0.88 -0.3 252,447 + BBR_RS11180 0.89 -0.4 252,457 + +0.0 252,478 + +0.3 252,478 + +0.1 252,478 + -0.9 252,486 - -0.2 252,486 - -1.0 252,486 - +0.2 252,486 - -0.2 252,488 + -0.3 252,503 + -0.4 252,511 - -1.0 252,524 + -1.3 252,524 + -0.4 252,527 - -0.4 252,527 - -1.2 252,534 + -1.9 252,534 + -0.5 252,557 - -0.2 252,558 + -0.1 252,571 + -0.7 252,602 - -0.2 252,623 - -0.0 252,623 - -0.5 252,660 + +0.6 252,667 + -0.2 252,667 + +1.0 252,667 + +0.3 254,714 - BBR_RS11185 0.67 -1.3 255,225 + BBR_RS11185 0.84 -1.4 255,233 - BBR_RS11185 0.84 -3.2 255,265 - BBR_RS11185 0.85 +0.7 255,306 + BBR_RS11185 0.86 -3.0 255,371 + BBR_RS11185 0.88 -3.0 255,455 + +0.2 255,495 + -0.1 255,495 + -0.5 255,503 - -0.0 255,511 + +0.9 255,537 + +0.9 255,540 - -0.8 255,572 + +0.3 255,572 + +0.0 255,605 + -0.0 255,605 + +0.8 255,605 + -0.1 255,613 - -0.5 255,613 - -0.3 255,615 + -0.3 255,638 - -1.2 255,640 + +0.1 255,661 + -0.1 255,671 + -0.1 255,697 - -3.2 255,704 - +0.8 255,794 + -0.0 255,799 + +0.2 255,799 + -0.1 255,810 + +0.5 255,812 + +0.6 255,823 + +0.6 255,823 + -0.8 255,831 - -1.7 255,832 - -0.1 255,838 + +0.6 255,865 - +0.7 255,867 + +0.0 255,867 + -0.7 255,875 - -1.9 255,899 + +0.1 255,899 + -0.1 255,918 + -0.3 255,923 + -0.3 255,926 - +0.6 255,926 - +1.1 255,982 + -0.2 255,991 - +0.1 256,011 + -0.1 256,011 + -0.2 256,017 - +0.5 256,028 + -0.9 256,029 + -0.5 256,037 - -0.9 256,659 + -0.5 256,673 + +0.2
Or see this region's nucleotide sequence