Experiment: MoYLS4 with D-Cycloserine 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2268 and DvMF_2269 are separated by 207 nucleotides DvMF_2269 and DvMF_2270 are separated by 18 nucleotides DvMF_2270 and DvMF_2271 are separated by 128 nucleotides
DvMF_2268: DvMF_2268 - branched-chain amino acid aminotransferase (RefSeq), at 2,850,088 to 2,851,011
_2268
DvMF_2269: DvMF_2269 - DNA polymerase III, subunits gamma and tau (RefSeq), at 2,851,219 to 2,853,285
_2269
DvMF_2270: DvMF_2270 - hypothetical protein (RefSeq), at 2,853,304 to 2,853,615
_2270
DvMF_2271: DvMF_2271 - recombination protein RecR (RefSeq), at 2,853,744 to 2,854,349
_2271
Position (kb)
2851
2852
2853
2854 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2850.691 kb on - strand, within DvMF_2268 at 2850.846 kb on - strand, within DvMF_2268 at 2850.846 kb on - strand, within DvMF_2268 at 2851.005 kb on + strand at 2851.019 kb on - strand at 2853.726 kb on + strand at 2853.885 kb on + strand, within DvMF_2271 at 2853.947 kb on + strand, within DvMF_2271 at 2854.032 kb on - strand, within DvMF_2271 at 2854.050 kb on + strand, within DvMF_2271 at 2854.164 kb on + strand, within DvMF_2271 at 2854.172 kb on - strand, within DvMF_2271 at 2854.177 kb on + strand, within DvMF_2271 at 2854.262 kb on + strand, within DvMF_2271
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with D-Cycloserine 0.25 mg/ml remove 2,850,691 - DvMF_2268 0.65 -0.6 2,850,846 - DvMF_2268 0.82 -1.2 2,850,846 - DvMF_2268 0.82 -1.0 2,851,005 + -0.1 2,851,019 - -0.7 2,853,726 + -0.6 2,853,885 + DvMF_2271 0.23 -4.6 2,853,947 + DvMF_2271 0.33 -5.3 2,854,032 - DvMF_2271 0.48 -4.5 2,854,050 + DvMF_2271 0.50 -4.1 2,854,164 + DvMF_2271 0.69 -4.9 2,854,172 - DvMF_2271 0.71 -3.6 2,854,177 + DvMF_2271 0.71 -3.6 2,854,262 + DvMF_2271 0.85 -3.2
Or see this region's nucleotide sequence