Experiment: MoYLS4 with D-Cycloserine 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1090 and DvMF_1091 are separated by 817 nucleotides DvMF_1091 and DvMF_1092 are separated by 168 nucleotides DvMF_1092 and DvMF_1093 overlap by 10 nucleotides
DvMF_1090: DvMF_1090 - hypothetical protein (RefSeq), at 1,332,380 to 1,333,438
_1090
DvMF_1091: DvMF_1091 - single-strand binding protein/Primosomal replication protein n (RefSeq), at 1,334,256 to 1,334,786
_1091
DvMF_1092: DvMF_1092 - hypothetical protein (RefSeq), at 1,334,955 to 1,335,386
_1092
DvMF_1093: DvMF_1093 - hypothetical protein (RefSeq), at 1,335,377 to 1,335,685
_1093
Position (kb)
1334
1335 Strain fitness (log2 ratio)
-2
-1
0
1 at 1333.283 kb on - strand, within DvMF_1090 at 1333.687 kb on - strand at 1333.738 kb on - strand at 1333.782 kb on + strand at 1333.839 kb on - strand at 1333.895 kb on + strand at 1333.926 kb on - strand at 1334.261 kb on - strand at 1334.367 kb on - strand, within DvMF_1091 at 1334.437 kb on + strand, within DvMF_1091 at 1334.585 kb on + strand, within DvMF_1091 at 1334.762 kb on + strand at 1334.812 kb on + strand at 1334.985 kb on + strand at 1335.220 kb on + strand, within DvMF_1092 at 1335.256 kb on + strand, within DvMF_1092 at 1335.274 kb on + strand, within DvMF_1092 at 1335.564 kb on - strand, within DvMF_1093 at 1335.565 kb on + strand, within DvMF_1093
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with D-Cycloserine 0.25 mg/ml remove 1,333,283 - DvMF_1090 0.85 -0.3 1,333,687 - -0.4 1,333,738 - -1.8 1,333,782 + -0.1 1,333,839 - -0.6 1,333,895 + -0.4 1,333,926 - -1.1 1,334,261 - -0.7 1,334,367 - DvMF_1091 0.21 -0.1 1,334,437 + DvMF_1091 0.34 -0.6 1,334,585 + DvMF_1091 0.62 -0.6 1,334,762 + -2.2 1,334,812 + -0.6 1,334,985 + +0.7 1,335,220 + DvMF_1092 0.61 -1.7 1,335,256 + DvMF_1092 0.70 -1.1 1,335,274 + DvMF_1092 0.74 -0.3 1,335,564 - DvMF_1093 0.61 -0.6 1,335,565 + DvMF_1093 0.61 +0.1
Or see this region's nucleotide sequence