Experiment: acetate 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1901 and Shew_1902 are separated by 111 nucleotides Shew_1902 and Shew_1903 are separated by 17 nucleotides Shew_1903 and Shew_1904 are separated by 127 nucleotides
Shew_1901: Shew_1901 - hypothetical protein (RefSeq), at 2,196,652 to 2,196,987
_1901
Shew_1902: Shew_1902 - NUDIX hydrolase (RefSeq), at 2,197,099 to 2,197,668
_1902
Shew_1903: Shew_1903 - para-aminobenzoate synthase, subunit I (RefSeq), at 2,197,686 to 2,199,119
_1903
Shew_1904: Shew_1904 - tartrate/fumarate subfamily Fe-S type hydro-lyase alpha subunit (RefSeq), at 2,199,247 to 2,200,770
_1904
Position (kb)
2197
2198
2199
2200 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2196.752 kb on - strand, within Shew_1901 at 2196.841 kb on + strand, within Shew_1901 at 2197.052 kb on - strand at 2197.052 kb on - strand at 2197.758 kb on - strand at 2198.410 kb on - strand, within Shew_1903 at 2198.630 kb on - strand, within Shew_1903 at 2199.025 kb on - strand at 2199.524 kb on - strand, within Shew_1904 at 2199.929 kb on - strand, within Shew_1904
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 25 mM remove 2,196,752 - Shew_1901 0.30 -0.2 2,196,841 + Shew_1901 0.56 -0.0 2,197,052 - +0.4 2,197,052 - +0.3 2,197,758 - +0.0 2,198,410 - Shew_1903 0.50 -0.1 2,198,630 - Shew_1903 0.66 -1.1 2,199,025 - +0.2 2,199,524 - Shew_1904 0.18 -3.1 2,199,929 - Shew_1904 0.45 -1.0
Or see this region's nucleotide sequence