Experiment: R2A with Chloride 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS10845 and LRK54_RS10850 are separated by 10 nucleotides LRK54_RS10850 and LRK54_RS10855 are separated by 296 nucleotides LRK54_RS10855 and LRK54_RS10860 are separated by 9 nucleotides
LRK54_RS10845: LRK54_RS10845 - 50S ribosomal protein L33, at 2,399,355 to 2,399,522
_RS10845
LRK54_RS10850: LRK54_RS10850 - 50S ribosomal protein L28, at 2,399,533 to 2,399,769
_RS10850
LRK54_RS10855: LRK54_RS10855 - protein-export chaperone SecB, at 2,400,066 to 2,400,569
_RS10855
LRK54_RS10860: LRK54_RS10860 - NAD(P)-dependent glycerol-3-phosphate dehydrogenase, at 2,400,579 to 2,401,604
_RS10860
Position (kb)
2400
2401 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2399.070 kb on + strand at 2399.070 kb on + strand at 2399.070 kb on + strand at 2399.070 kb on + strand at 2399.070 kb on + strand at 2399.070 kb on + strand at 2399.070 kb on + strand at 2399.070 kb on + strand at 2399.070 kb on + strand at 2399.071 kb on - strand at 2399.071 kb on - strand at 2399.071 kb on - strand at 2399.071 kb on - strand at 2399.071 kb on - strand at 2399.251 kb on + strand at 2399.252 kb on - strand at 2399.377 kb on + strand, within LRK54_RS10845 at 2399.377 kb on + strand, within LRK54_RS10845 at 2399.377 kb on + strand, within LRK54_RS10845 at 2399.377 kb on + strand, within LRK54_RS10845 at 2399.377 kb on + strand, within LRK54_RS10845 at 2399.377 kb on + strand, within LRK54_RS10845 at 2399.377 kb on + strand, within LRK54_RS10845 at 2399.377 kb on + strand, within LRK54_RS10845 at 2399.377 kb on + strand, within LRK54_RS10845 at 2399.377 kb on + strand, within LRK54_RS10845 at 2399.378 kb on - strand, within LRK54_RS10845 at 2399.378 kb on - strand, within LRK54_RS10845 at 2399.378 kb on - strand, within LRK54_RS10845 at 2399.378 kb on - strand, within LRK54_RS10845 at 2399.378 kb on - strand, within LRK54_RS10845 at 2399.378 kb on - strand, within LRK54_RS10845 at 2399.378 kb on - strand, within LRK54_RS10845 at 2399.378 kb on - strand, within LRK54_RS10845 at 2399.378 kb on - strand, within LRK54_RS10845 at 2399.407 kb on + strand, within LRK54_RS10845 at 2399.407 kb on + strand, within LRK54_RS10845 at 2399.407 kb on + strand, within LRK54_RS10845 at 2399.407 kb on + strand, within LRK54_RS10845 at 2399.407 kb on + strand, within LRK54_RS10845 at 2399.407 kb on + strand, within LRK54_RS10845 at 2399.407 kb on + strand, within LRK54_RS10845 at 2399.408 kb on - strand, within LRK54_RS10845 at 2399.408 kb on - strand, within LRK54_RS10845 at 2399.408 kb on - strand, within LRK54_RS10845 at 2399.408 kb on - strand, within LRK54_RS10845 at 2399.408 kb on - strand, within LRK54_RS10845 at 2399.408 kb on - strand, within LRK54_RS10845 at 2399.408 kb on - strand, within LRK54_RS10845 at 2399.461 kb on + strand, within LRK54_RS10845 at 2399.461 kb on + strand, within LRK54_RS10845 at 2399.461 kb on + strand, within LRK54_RS10845 at 2399.461 kb on + strand, within LRK54_RS10845 at 2399.461 kb on + strand, within LRK54_RS10845 at 2399.461 kb on + strand, within LRK54_RS10845 at 2399.461 kb on + strand, within LRK54_RS10845 at 2399.461 kb on + strand, within LRK54_RS10845 at 2399.461 kb on + strand, within LRK54_RS10845 at 2399.461 kb on + strand, within LRK54_RS10845 at 2399.461 kb on + strand, within LRK54_RS10845 at 2399.462 kb on - strand, within LRK54_RS10845 at 2399.462 kb on - strand, within LRK54_RS10845 at 2399.462 kb on - strand, within LRK54_RS10845 at 2399.462 kb on - strand, within LRK54_RS10845 at 2399.462 kb on - strand, within LRK54_RS10845 at 2399.462 kb on - strand, within LRK54_RS10845 at 2399.462 kb on - strand, within LRK54_RS10845 at 2399.462 kb on - strand, within LRK54_RS10845 at 2399.462 kb on - strand, within LRK54_RS10845 at 2399.462 kb on - strand, within LRK54_RS10845 at 2399.462 kb on - strand, within LRK54_RS10845 at 2399.462 kb on - strand, within LRK54_RS10845 at 2399.517 kb on - strand at 2399.517 kb on - strand at 2399.792 kb on - strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.013 kb on + strand at 2400.014 kb on - strand at 2400.014 kb on - strand at 2400.036 kb on + strand at 2400.036 kb on + strand at 2400.036 kb on + strand at 2400.036 kb on + strand at 2400.036 kb on + strand at 2400.138 kb on + strand, within LRK54_RS10855 at 2400.138 kb on + strand, within LRK54_RS10855 at 2400.138 kb on + strand, within LRK54_RS10855 at 2400.138 kb on + strand, within LRK54_RS10855 at 2400.264 kb on + strand, within LRK54_RS10855 at 2400.264 kb on + strand, within LRK54_RS10855 at 2400.264 kb on + strand, within LRK54_RS10855 at 2400.264 kb on + strand, within LRK54_RS10855 at 2400.264 kb on + strand, within LRK54_RS10855 at 2400.264 kb on + strand, within LRK54_RS10855 at 2400.318 kb on + strand, within LRK54_RS10855 at 2400.318 kb on + strand, within LRK54_RS10855 at 2400.399 kb on + strand, within LRK54_RS10855 at 2400.399 kb on + strand, within LRK54_RS10855 at 2400.399 kb on + strand, within LRK54_RS10855 at 2400.399 kb on + strand, within LRK54_RS10855 at 2400.399 kb on + strand, within LRK54_RS10855 at 2400.423 kb on + strand, within LRK54_RS10855
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 250 mM remove 2,399,070 + -0.2 2,399,070 + +0.2 2,399,070 + +0.5 2,399,070 + -0.6 2,399,070 + +1.8 2,399,070 + -0.2 2,399,070 + +0.2 2,399,070 + +0.4 2,399,070 + -0.6 2,399,071 - -0.1 2,399,071 - -0.5 2,399,071 - -1.5 2,399,071 - -0.4 2,399,071 - -0.8 2,399,251 + -0.1 2,399,252 - -0.7 2,399,377 + LRK54_RS10845 0.13 +1.8 2,399,377 + LRK54_RS10845 0.13 +0.1 2,399,377 + LRK54_RS10845 0.13 -0.1 2,399,377 + LRK54_RS10845 0.13 +0.1 2,399,377 + LRK54_RS10845 0.13 -0.5 2,399,377 + LRK54_RS10845 0.13 -0.5 2,399,377 + LRK54_RS10845 0.13 -0.1 2,399,377 + LRK54_RS10845 0.13 +0.9 2,399,377 + LRK54_RS10845 0.13 -1.0 2,399,377 + LRK54_RS10845 0.13 -1.1 2,399,378 - LRK54_RS10845 0.14 +0.7 2,399,378 - LRK54_RS10845 0.14 +0.4 2,399,378 - LRK54_RS10845 0.14 -0.8 2,399,378 - LRK54_RS10845 0.14 +2.2 2,399,378 - LRK54_RS10845 0.14 +1.0 2,399,378 - LRK54_RS10845 0.14 -0.6 2,399,378 - LRK54_RS10845 0.14 +1.6 2,399,378 - LRK54_RS10845 0.14 -1.2 2,399,378 - LRK54_RS10845 0.14 +1.0 2,399,407 + LRK54_RS10845 0.31 +1.8 2,399,407 + LRK54_RS10845 0.31 -0.2 2,399,407 + LRK54_RS10845 0.31 -0.5 2,399,407 + LRK54_RS10845 0.31 -0.5 2,399,407 + LRK54_RS10845 0.31 +1.5 2,399,407 + LRK54_RS10845 0.31 +1.7 2,399,407 + LRK54_RS10845 0.31 +0.2 2,399,408 - LRK54_RS10845 0.32 -0.1 2,399,408 - LRK54_RS10845 0.32 +0.9 2,399,408 - LRK54_RS10845 0.32 +1.2 2,399,408 - LRK54_RS10845 0.32 +1.5 2,399,408 - LRK54_RS10845 0.32 +3.2 2,399,408 - LRK54_RS10845 0.32 +1.0 2,399,408 - LRK54_RS10845 0.32 +0.8 2,399,461 + LRK54_RS10845 0.63 -0.2 2,399,461 + LRK54_RS10845 0.63 +0.3 2,399,461 + LRK54_RS10845 0.63 +0.5 2,399,461 + LRK54_RS10845 0.63 +2.0 2,399,461 + LRK54_RS10845 0.63 +2.6 2,399,461 + LRK54_RS10845 0.63 +0.0 2,399,461 + LRK54_RS10845 0.63 -0.0 2,399,461 + LRK54_RS10845 0.63 -0.5 2,399,461 + LRK54_RS10845 0.63 -0.5 2,399,461 + LRK54_RS10845 0.63 +0.2 2,399,461 + LRK54_RS10845 0.63 +2.0 2,399,462 - LRK54_RS10845 0.64 +2.1 2,399,462 - LRK54_RS10845 0.64 +1.2 2,399,462 - LRK54_RS10845 0.64 +0.2 2,399,462 - LRK54_RS10845 0.64 -0.5 2,399,462 - LRK54_RS10845 0.64 +1.6 2,399,462 - LRK54_RS10845 0.64 +1.8 2,399,462 - LRK54_RS10845 0.64 +0.8 2,399,462 - LRK54_RS10845 0.64 +1.3 2,399,462 - LRK54_RS10845 0.64 +0.9 2,399,462 - LRK54_RS10845 0.64 +2.2 2,399,462 - LRK54_RS10845 0.64 +2.2 2,399,462 - LRK54_RS10845 0.64 +1.3 2,399,517 - +0.4 2,399,517 - +2.5 2,399,792 - +0.8 2,400,013 + +1.8 2,400,013 + +1.6 2,400,013 + +0.7 2,400,013 + -2.0 2,400,013 + -0.5 2,400,013 + -0.2 2,400,013 + +0.1 2,400,013 + +1.9 2,400,013 + +0.0 2,400,013 + +1.2 2,400,013 + +1.8 2,400,013 + -1.0 2,400,013 + +0.1 2,400,013 + -0.5 2,400,013 + -1.8 2,400,013 + +0.8 2,400,013 + +0.2 2,400,013 + -1.0 2,400,013 + +0.8 2,400,013 + -1.8 2,400,013 + +0.8 2,400,013 + +0.3 2,400,013 + +0.5 2,400,013 + +0.2 2,400,013 + +2.2 2,400,013 + +1.6 2,400,013 + -0.5 2,400,013 + -0.5 2,400,013 + -0.2 2,400,013 + -0.5 2,400,013 + -0.5 2,400,013 + -0.7 2,400,013 + -2.4 2,400,013 + +0.2 2,400,013 + +0.1 2,400,013 + +0.2 2,400,013 + +0.5 2,400,013 + -1.5 2,400,013 + +1.4 2,400,013 + -1.2 2,400,013 + -0.5 2,400,013 + -0.2 2,400,013 + +0.2 2,400,013 + +0.5 2,400,013 + +0.8 2,400,013 + -0.6 2,400,013 + -0.8 2,400,013 + -1.2 2,400,013 + -0.1 2,400,013 + -0.8 2,400,013 + +0.3 2,400,013 + -0.8 2,400,013 + +0.3 2,400,013 + +2.8 2,400,013 + -0.3 2,400,013 + -0.7 2,400,013 + +0.1 2,400,013 + -1.0 2,400,013 + -0.3 2,400,013 + -1.0 2,400,013 + +0.6 2,400,013 + -0.2 2,400,013 + +1.8 2,400,013 + +0.2 2,400,014 - +1.8 2,400,014 - -0.5 2,400,036 + -2.3 2,400,036 + +0.3 2,400,036 + -0.8 2,400,036 + -1.2 2,400,036 + -1.0 2,400,138 + LRK54_RS10855 0.14 -0.8 2,400,138 + LRK54_RS10855 0.14 -3.1 2,400,138 + LRK54_RS10855 0.14 -1.9 2,400,138 + LRK54_RS10855 0.14 -0.8 2,400,264 + LRK54_RS10855 0.39 +0.2 2,400,264 + LRK54_RS10855 0.39 +0.8 2,400,264 + LRK54_RS10855 0.39 -1.2 2,400,264 + LRK54_RS10855 0.39 -0.5 2,400,264 + LRK54_RS10855 0.39 -2.0 2,400,264 + LRK54_RS10855 0.39 -0.2 2,400,318 + LRK54_RS10855 0.50 -0.8 2,400,318 + LRK54_RS10855 0.50 -2.3 2,400,399 + LRK54_RS10855 0.66 +1.8 2,400,399 + LRK54_RS10855 0.66 +0.8 2,400,399 + LRK54_RS10855 0.66 -1.3 2,400,399 + LRK54_RS10855 0.66 -0.8 2,400,399 + LRK54_RS10855 0.66 -1.0 2,400,423 + LRK54_RS10855 0.71 -1.2
Or see this region's nucleotide sequence