Experiment: R2A with Chloride 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS02455 and LRK54_RS02460 overlap by 4 nucleotides LRK54_RS02460 and LRK54_RS02465 overlap by 4 nucleotides LRK54_RS02465 and LRK54_RS02470 are separated by 92 nucleotides LRK54_RS02470 and LRK54_RS02475 are separated by 14 nucleotides
LRK54_RS02455: LRK54_RS02455 - squalene/phytoene synthase family protein, at 533,489 to 534,343
_RS02455
LRK54_RS02460: LRK54_RS02460 - phosphoglycolate phosphatase, at 534,340 to 535,020
_RS02460
LRK54_RS02465: LRK54_RS02465 - bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG, at 535,017 to 535,721
_RS02465
LRK54_RS02470: LRK54_RS02470 - YciI family protein, at 535,814 to 536,068
_RS02470
LRK54_RS02475: LRK54_RS02475 - TRZ/ATZ family hydrolase, at 536,083 to 537,411
_RS02475
Position (kb) 535
536 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 534.020 kb on + strand, within LRK54_RS02455 at 534.440 kb on + strand, within LRK54_RS02460 at 534.441 kb on - strand, within LRK54_RS02460 at 534.441 kb on - strand, within LRK54_RS02460 at 534.441 kb on - strand, within LRK54_RS02460 at 534.441 kb on - strand, within LRK54_RS02460 at 534.441 kb on - strand, within LRK54_RS02460 at 534.441 kb on - strand, within LRK54_RS02460 at 534.464 kb on + strand, within LRK54_RS02460 at 534.464 kb on + strand, within LRK54_RS02460 at 534.464 kb on + strand, within LRK54_RS02460 at 534.464 kb on + strand, within LRK54_RS02460 at 534.464 kb on + strand, within LRK54_RS02460 at 534.464 kb on + strand, within LRK54_RS02460 at 534.465 kb on + strand, within LRK54_RS02460 at 534.465 kb on - strand, within LRK54_RS02460 at 534.465 kb on - strand, within LRK54_RS02460 at 534.465 kb on - strand, within LRK54_RS02460 at 534.765 kb on + strand, within LRK54_RS02460 at 534.765 kb on + strand, within LRK54_RS02460 at 534.765 kb on + strand, within LRK54_RS02460 at 534.765 kb on + strand, within LRK54_RS02460 at 534.765 kb on + strand, within LRK54_RS02460 at 534.765 kb on + strand, within LRK54_RS02460 at 534.765 kb on + strand, within LRK54_RS02460 at 534.765 kb on + strand, within LRK54_RS02460 at 534.766 kb on - strand, within LRK54_RS02460 at 534.766 kb on - strand, within LRK54_RS02460 at 534.766 kb on - strand, within LRK54_RS02460 at 534.766 kb on - strand, within LRK54_RS02460 at 534.766 kb on - strand, within LRK54_RS02460 at 534.766 kb on - strand, within LRK54_RS02460 at 534.766 kb on - strand, within LRK54_RS02460 at 534.766 kb on - strand, within LRK54_RS02460 at 534.776 kb on + strand, within LRK54_RS02460 at 534.776 kb on + strand, within LRK54_RS02460 at 534.776 kb on + strand, within LRK54_RS02460 at 534.776 kb on + strand, within LRK54_RS02460 at 534.776 kb on + strand, within LRK54_RS02460 at 534.776 kb on + strand, within LRK54_RS02460 at 534.776 kb on + strand, within LRK54_RS02460 at 534.776 kb on + strand, within LRK54_RS02460 at 534.776 kb on + strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.777 kb on - strand, within LRK54_RS02460 at 534.788 kb on + strand, within LRK54_RS02460 at 534.788 kb on + strand, within LRK54_RS02460 at 534.788 kb on + strand, within LRK54_RS02460 at 534.788 kb on + strand, within LRK54_RS02460 at 534.788 kb on + strand, within LRK54_RS02460 at 534.789 kb on - strand, within LRK54_RS02460 at 534.789 kb on - strand, within LRK54_RS02460 at 534.789 kb on - strand, within LRK54_RS02460 at 534.789 kb on - strand, within LRK54_RS02460 at 534.789 kb on - strand, within LRK54_RS02460 at 534.789 kb on - strand, within LRK54_RS02460 at 534.790 kb on - strand, within LRK54_RS02460 at 534.796 kb on + strand, within LRK54_RS02460 at 534.797 kb on - strand, within LRK54_RS02460 at 534.797 kb on - strand, within LRK54_RS02460 at 534.797 kb on - strand, within LRK54_RS02460 at 534.890 kb on + strand, within LRK54_RS02460 at 534.890 kb on + strand, within LRK54_RS02460 at 534.890 kb on + strand, within LRK54_RS02460 at 534.891 kb on - strand, within LRK54_RS02460 at 534.891 kb on - strand, within LRK54_RS02460 at 534.891 kb on - strand, within LRK54_RS02460 at 534.891 kb on - strand, within LRK54_RS02460 at 534.941 kb on + strand, within LRK54_RS02460 at 534.941 kb on + strand, within LRK54_RS02460 at 534.941 kb on + strand, within LRK54_RS02460 at 534.942 kb on - strand, within LRK54_RS02460 at 534.942 kb on - strand, within LRK54_RS02460 at 534.942 kb on - strand, within LRK54_RS02460 at 534.942 kb on - strand, within LRK54_RS02460 at 534.942 kb on - strand, within LRK54_RS02460 at 534.942 kb on - strand, within LRK54_RS02460 at 534.942 kb on - strand, within LRK54_RS02460 at 535.750 kb on + strand at 535.750 kb on + strand at 535.751 kb on - strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.833 kb on + strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.834 kb on - strand at 535.923 kb on + strand, within LRK54_RS02470 at 535.923 kb on + strand, within LRK54_RS02470 at 535.923 kb on + strand, within LRK54_RS02470 at 535.923 kb on + strand, within LRK54_RS02470 at 535.923 kb on + strand, within LRK54_RS02470 at 535.924 kb on - strand, within LRK54_RS02470 at 535.924 kb on - strand, within LRK54_RS02470 at 535.924 kb on - strand, within LRK54_RS02470 at 535.924 kb on - strand, within LRK54_RS02470 at 535.924 kb on - strand, within LRK54_RS02470 at 535.924 kb on - strand, within LRK54_RS02470 at 535.924 kb on - strand, within LRK54_RS02470 at 535.924 kb on - strand, within LRK54_RS02470 at 535.924 kb on - strand, within LRK54_RS02470 at 536.059 kb on - strand at 536.097 kb on + strand at 536.097 kb on + strand at 536.097 kb on + strand at 536.097 kb on + strand at 536.097 kb on + strand at 536.097 kb on + strand at 536.097 kb on + strand at 536.097 kb on + strand at 536.097 kb on + strand at 536.097 kb on + strand at 536.097 kb on + strand at 536.097 kb on + strand at 536.097 kb on + strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.098 kb on - strand at 536.222 kb on + strand, within LRK54_RS02475 at 536.222 kb on + strand, within LRK54_RS02475 at 536.222 kb on + strand, within LRK54_RS02475 at 536.222 kb on + strand, within LRK54_RS02475 at 536.222 kb on + strand, within LRK54_RS02475 at 536.222 kb on + strand, within LRK54_RS02475 at 536.222 kb on + strand, within LRK54_RS02475 at 536.222 kb on + strand, within LRK54_RS02475 at 536.222 kb on + strand, within LRK54_RS02475 at 536.223 kb on - strand, within LRK54_RS02475 at 536.223 kb on - strand, within LRK54_RS02475 at 536.223 kb on - strand, within LRK54_RS02475 at 536.223 kb on - strand, within LRK54_RS02475 at 536.223 kb on - strand, within LRK54_RS02475 at 536.223 kb on - strand, within LRK54_RS02475 at 536.223 kb on - strand, within LRK54_RS02475
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 250 mM remove 534,020 + LRK54_RS02455 0.62 -0.2 534,440 + LRK54_RS02460 0.15 +0.2 534,441 - LRK54_RS02460 0.15 -0.8 534,441 - LRK54_RS02460 0.15 -2.6 534,441 - LRK54_RS02460 0.15 -2.3 534,441 - LRK54_RS02460 0.15 -2.7 534,441 - LRK54_RS02460 0.15 -0.2 534,441 - LRK54_RS02460 0.15 -0.2 534,464 + LRK54_RS02460 0.18 +1.2 534,464 + LRK54_RS02460 0.18 -1.6 534,464 + LRK54_RS02460 0.18 -1.4 534,464 + LRK54_RS02460 0.18 -1.2 534,464 + LRK54_RS02460 0.18 +0.1 534,464 + LRK54_RS02460 0.18 -0.8 534,465 + LRK54_RS02460 0.18 -2.2 534,465 - LRK54_RS02460 0.18 -1.5 534,465 - LRK54_RS02460 0.18 -0.5 534,465 - LRK54_RS02460 0.18 -1.8 534,765 + LRK54_RS02460 0.62 +0.0 534,765 + LRK54_RS02460 0.62 -2.0 534,765 + LRK54_RS02460 0.62 -1.2 534,765 + LRK54_RS02460 0.62 -0.5 534,765 + LRK54_RS02460 0.62 -0.4 534,765 + LRK54_RS02460 0.62 +0.2 534,765 + LRK54_RS02460 0.62 +0.8 534,765 + LRK54_RS02460 0.62 -1.4 534,766 - LRK54_RS02460 0.63 +0.8 534,766 - LRK54_RS02460 0.63 -0.6 534,766 - LRK54_RS02460 0.63 -1.1 534,766 - LRK54_RS02460 0.63 -2.4 534,766 - LRK54_RS02460 0.63 -1.0 534,766 - LRK54_RS02460 0.63 -0.2 534,766 - LRK54_RS02460 0.63 -0.5 534,766 - LRK54_RS02460 0.63 -2.4 534,776 + LRK54_RS02460 0.64 -0.5 534,776 + LRK54_RS02460 0.64 -0.2 534,776 + LRK54_RS02460 0.64 -0.8 534,776 + LRK54_RS02460 0.64 -1.0 534,776 + LRK54_RS02460 0.64 +0.2 534,776 + LRK54_RS02460 0.64 -2.6 534,776 + LRK54_RS02460 0.64 -2.2 534,776 + LRK54_RS02460 0.64 -1.9 534,776 + LRK54_RS02460 0.64 -0.8 534,777 - LRK54_RS02460 0.64 -2.6 534,777 - LRK54_RS02460 0.64 -0.5 534,777 - LRK54_RS02460 0.64 -1.9 534,777 - LRK54_RS02460 0.64 +1.8 534,777 - LRK54_RS02460 0.64 -2.1 534,777 - LRK54_RS02460 0.64 -3.7 534,777 - LRK54_RS02460 0.64 -1.5 534,777 - LRK54_RS02460 0.64 -0.5 534,777 - LRK54_RS02460 0.64 -1.0 534,777 - LRK54_RS02460 0.64 -2.2 534,777 - LRK54_RS02460 0.64 -2.0 534,777 - LRK54_RS02460 0.64 -2.3 534,777 - LRK54_RS02460 0.64 -1.8 534,777 - LRK54_RS02460 0.64 -1.0 534,777 - LRK54_RS02460 0.64 -0.2 534,777 - LRK54_RS02460 0.64 -1.0 534,777 - LRK54_RS02460 0.64 -2.8 534,788 + LRK54_RS02460 0.66 +0.2 534,788 + LRK54_RS02460 0.66 -1.6 534,788 + LRK54_RS02460 0.66 -2.6 534,788 + LRK54_RS02460 0.66 -1.0 534,788 + LRK54_RS02460 0.66 -1.9 534,789 - LRK54_RS02460 0.66 -1.8 534,789 - LRK54_RS02460 0.66 +0.2 534,789 - LRK54_RS02460 0.66 +0.8 534,789 - LRK54_RS02460 0.66 -0.9 534,789 - LRK54_RS02460 0.66 -1.6 534,789 - LRK54_RS02460 0.66 -2.6 534,790 - LRK54_RS02460 0.66 -0.5 534,796 + LRK54_RS02460 0.67 -1.6 534,797 - LRK54_RS02460 0.67 -1.5 534,797 - LRK54_RS02460 0.67 +0.5 534,797 - LRK54_RS02460 0.67 +0.8 534,890 + LRK54_RS02460 0.81 -1.9 534,890 + LRK54_RS02460 0.81 -0.2 534,890 + LRK54_RS02460 0.81 -1.0 534,891 - LRK54_RS02460 0.81 -2.0 534,891 - LRK54_RS02460 0.81 -0.8 534,891 - LRK54_RS02460 0.81 +0.1 534,891 - LRK54_RS02460 0.81 -1.2 534,941 + LRK54_RS02460 0.88 -1.2 534,941 + LRK54_RS02460 0.88 +0.8 534,941 + LRK54_RS02460 0.88 -2.5 534,942 - LRK54_RS02460 0.88 +0.8 534,942 - LRK54_RS02460 0.88 -2.0 534,942 - LRK54_RS02460 0.88 -2.8 534,942 - LRK54_RS02460 0.88 -0.2 534,942 - LRK54_RS02460 0.88 +1.2 534,942 - LRK54_RS02460 0.88 +0.0 534,942 - LRK54_RS02460 0.88 -1.4 535,750 + -0.3 535,750 + -1.0 535,751 - -1.4 535,833 + -0.7 535,833 + -0.7 535,833 + -0.2 535,833 + +0.2 535,833 + -1.1 535,833 + +1.4 535,833 + -0.2 535,833 + +0.6 535,833 + -2.0 535,833 + +0.5 535,833 + -0.1 535,833 + -1.6 535,833 + +0.6 535,833 + -1.8 535,834 - -0.4 535,834 - +1.8 535,834 - +1.1 535,834 - +0.2 535,834 - +0.3 535,834 - -0.8 535,834 - +0.1 535,834 - -0.0 535,834 - -1.2 535,834 - +1.4 535,834 - -0.2 535,834 - -0.6 535,834 - -2.0 535,834 - +0.8 535,834 - -0.0 535,834 - +0.9 535,834 - +2.6 535,834 - +0.8 535,834 - +0.0 535,923 + LRK54_RS02470 0.43 +0.8 535,923 + LRK54_RS02470 0.43 +0.4 535,923 + LRK54_RS02470 0.43 -0.9 535,923 + LRK54_RS02470 0.43 -0.4 535,923 + LRK54_RS02470 0.43 +2.0 535,924 - LRK54_RS02470 0.43 -0.1 535,924 - LRK54_RS02470 0.43 +1.8 535,924 - LRK54_RS02470 0.43 +1.1 535,924 - LRK54_RS02470 0.43 -0.8 535,924 - LRK54_RS02470 0.43 -0.1 535,924 - LRK54_RS02470 0.43 -0.2 535,924 - LRK54_RS02470 0.43 +0.5 535,924 - LRK54_RS02470 0.43 -0.0 535,924 - LRK54_RS02470 0.43 +0.5 536,059 - +1.8 536,097 + -1.3 536,097 + +1.2 536,097 + +3.4 536,097 + +0.8 536,097 + +0.5 536,097 + +0.5 536,097 + +0.7 536,097 + +1.1 536,097 + +0.1 536,097 + +0.1 536,097 + +0.7 536,097 + +0.6 536,097 + -0.5 536,098 - -1.0 536,098 - -0.9 536,098 - +0.4 536,098 - -1.0 536,098 - +0.2 536,098 - +0.7 536,098 - +0.1 536,098 - +1.8 536,098 - -0.2 536,098 - +0.5 536,098 - +1.8 536,098 - -0.6 536,098 - -1.2 536,098 - -1.6 536,098 - +1.4 536,098 - -1.0 536,098 - +0.8 536,098 - -0.3 536,098 - -2.6 536,098 - +1.2 536,098 - +0.1 536,222 + LRK54_RS02475 0.10 +1.2 536,222 + LRK54_RS02475 0.10 +0.3 536,222 + LRK54_RS02475 0.10 -0.4 536,222 + LRK54_RS02475 0.10 +0.5 536,222 + LRK54_RS02475 0.10 -2.5 536,222 + LRK54_RS02475 0.10 -0.3 536,222 + LRK54_RS02475 0.10 -0.9 536,222 + LRK54_RS02475 0.10 -0.6 536,222 + LRK54_RS02475 0.10 +2.2 536,223 - LRK54_RS02475 0.11 +0.4 536,223 - LRK54_RS02475 0.11 -1.6 536,223 - LRK54_RS02475 0.11 +0.1 536,223 - LRK54_RS02475 0.11 +2.1 536,223 - LRK54_RS02475 0.11 +0.8 536,223 - LRK54_RS02475 0.11 -1.4 536,223 - LRK54_RS02475 0.11 -1.7
Or see this region's nucleotide sequence