Experiment: PYE with Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02222 and CCNA_02223 overlap by 4 nucleotides CCNA_02223 and CCNA_02224 overlap by 4 nucleotides CCNA_02224 and CCNA_02225 overlap by 4 nucleotides
CCNA_02222: CCNA_02222 - 5-methyltetrahydrofolate--homocysteine methyltransferase homocysteine-binding subunit, at 2,370,800 to 2,371,876
_02222
CCNA_02223: CCNA_02223 - beta-lactamase, type II, at 2,371,873 to 2,372,742
_02223
CCNA_02224: CCNA_02224 - methylenetetrahydrofolate reductase, at 2,372,739 to 2,373,683
_02224
CCNA_02225: CCNA_02225 - ArsR family/SAM-dependent transcriptional regulator, at 2,373,680 to 2,374,657
_02225
Position (kb)
2372
2373
2374 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2371.755 kb on + strand, within CCNA_02222 at 2371.763 kb on - strand, within CCNA_02222 at 2371.827 kb on - strand at 2371.838 kb on - strand at 2371.840 kb on - strand at 2371.915 kb on + strand at 2371.937 kb on + strand at 2372.003 kb on - strand, within CCNA_02223 at 2372.009 kb on + strand, within CCNA_02223 at 2372.020 kb on + strand, within CCNA_02223 at 2372.028 kb on - strand, within CCNA_02223 at 2372.051 kb on - strand, within CCNA_02223 at 2372.063 kb on + strand, within CCNA_02223 at 2372.071 kb on - strand, within CCNA_02223 at 2372.078 kb on + strand, within CCNA_02223 at 2372.117 kb on - strand, within CCNA_02223 at 2372.123 kb on + strand, within CCNA_02223 at 2372.177 kb on + strand, within CCNA_02223 at 2372.177 kb on + strand, within CCNA_02223 at 2372.210 kb on - strand, within CCNA_02223 at 2372.211 kb on - strand, within CCNA_02223 at 2372.225 kb on + strand, within CCNA_02223 at 2372.276 kb on - strand, within CCNA_02223 at 2372.276 kb on - strand, within CCNA_02223 at 2372.276 kb on - strand, within CCNA_02223 at 2372.276 kb on - strand, within CCNA_02223 at 2372.276 kb on - strand, within CCNA_02223 at 2372.276 kb on - strand, within CCNA_02223 at 2372.281 kb on - strand, within CCNA_02223 at 2372.281 kb on - strand, within CCNA_02223 at 2372.296 kb on - strand, within CCNA_02223 at 2372.390 kb on + strand, within CCNA_02223 at 2372.390 kb on + strand, within CCNA_02223 at 2372.405 kb on - strand, within CCNA_02223 at 2372.407 kb on + strand, within CCNA_02223 at 2372.414 kb on + strand, within CCNA_02223 at 2372.414 kb on + strand, within CCNA_02223 at 2372.422 kb on - strand, within CCNA_02223 at 2372.422 kb on - strand, within CCNA_02223 at 2372.422 kb on - strand, within CCNA_02223 at 2372.422 kb on - strand, within CCNA_02223 at 2372.472 kb on - strand, within CCNA_02223 at 2372.501 kb on + strand, within CCNA_02223 at 2372.502 kb on - strand, within CCNA_02223 at 2372.559 kb on - strand, within CCNA_02223 at 2372.564 kb on - strand, within CCNA_02223 at 2372.606 kb on + strand, within CCNA_02223 at 2372.615 kb on + strand, within CCNA_02223 at 2372.636 kb on + strand, within CCNA_02223 at 2372.636 kb on + strand, within CCNA_02223 at 2372.644 kb on - strand, within CCNA_02223 at 2372.690 kb on + strand at 2372.735 kb on - strand at 2372.750 kb on + strand at 2372.750 kb on + strand at 2372.771 kb on - strand at 2372.780 kb on + strand at 2372.781 kb on + strand at 2372.786 kb on + strand at 2372.794 kb on - strand at 2372.820 kb on + strand at 2372.827 kb on - strand at 2372.833 kb on + strand at 2372.853 kb on - strand, within CCNA_02224 at 2372.991 kb on + strand, within CCNA_02224 at 2373.024 kb on - strand, within CCNA_02224 at 2373.097 kb on + strand, within CCNA_02224 at 2373.196 kb on + strand, within CCNA_02224 at 2373.199 kb on - strand, within CCNA_02224 at 2373.199 kb on - strand, within CCNA_02224 at 2373.202 kb on + strand, within CCNA_02224 at 2373.202 kb on + strand, within CCNA_02224 at 2373.210 kb on - strand, within CCNA_02224 at 2373.214 kb on + strand, within CCNA_02224 at 2373.224 kb on + strand, within CCNA_02224 at 2373.241 kb on + strand, within CCNA_02224 at 2373.241 kb on + strand, within CCNA_02224 at 2373.249 kb on - strand, within CCNA_02224 at 2373.249 kb on - strand, within CCNA_02224 at 2373.254 kb on - strand, within CCNA_02224 at 2373.272 kb on - strand, within CCNA_02224 at 2373.273 kb on - strand, within CCNA_02224 at 2373.284 kb on + strand, within CCNA_02224 at 2373.414 kb on + strand, within CCNA_02224 at 2373.414 kb on + strand, within CCNA_02224 at 2373.422 kb on - strand, within CCNA_02224 at 2373.422 kb on - strand, within CCNA_02224 at 2373.439 kb on - strand, within CCNA_02224 at 2373.450 kb on - strand, within CCNA_02224 at 2373.500 kb on + strand, within CCNA_02224 at 2373.500 kb on + strand, within CCNA_02224 at 2373.508 kb on - strand, within CCNA_02224 at 2373.508 kb on - strand, within CCNA_02224 at 2373.508 kb on - strand, within CCNA_02224 at 2373.508 kb on - strand, within CCNA_02224 at 2373.510 kb on - strand, within CCNA_02224 at 2373.539 kb on + strand, within CCNA_02224 at 2373.543 kb on + strand, within CCNA_02224 at 2373.575 kb on - strand, within CCNA_02224 at 2373.583 kb on + strand, within CCNA_02224 at 2373.583 kb on + strand, within CCNA_02224 at 2373.583 kb on + strand, within CCNA_02224 at 2373.591 kb on - strand at 2373.667 kb on - strand at 2373.671 kb on + strand at 2373.692 kb on + strand at 2373.694 kb on + strand at 2373.700 kb on - strand at 2373.700 kb on - strand at 2373.734 kb on - strand at 2373.734 kb on - strand at 2373.787 kb on + strand, within CCNA_02225 at 2373.818 kb on + strand, within CCNA_02225 at 2373.856 kb on - strand, within CCNA_02225 at 2373.944 kb on - strand, within CCNA_02225 at 2373.971 kb on + strand, within CCNA_02225 at 2373.995 kb on - strand, within CCNA_02225 at 2374.100 kb on - strand, within CCNA_02225 at 2374.122 kb on - strand, within CCNA_02225 at 2374.249 kb on + strand, within CCNA_02225 at 2374.257 kb on - strand, within CCNA_02225 at 2374.299 kb on + strand, within CCNA_02225 at 2374.307 kb on - strand, within CCNA_02225 at 2374.317 kb on + strand, within CCNA_02225 at 2374.324 kb on - strand, within CCNA_02225 at 2374.353 kb on + strand, within CCNA_02225 at 2374.458 kb on - strand, within CCNA_02225 at 2374.458 kb on - strand, within CCNA_02225 at 2374.458 kb on - strand, within CCNA_02225 at 2374.458 kb on - strand, within CCNA_02225 at 2374.499 kb on - strand, within CCNA_02225 at 2374.539 kb on - strand, within CCNA_02225 at 2374.558 kb on - strand, within CCNA_02225
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Sisomicin 0.004 mg/ml remove 2,371,755 + CCNA_02222 0.89 +1.8 2,371,763 - CCNA_02222 0.89 -1.7 2,371,827 - +0.3 2,371,838 - +2.2 2,371,840 - -0.3 2,371,915 + -1.2 2,371,937 + -2.2 2,372,003 - CCNA_02223 0.15 -0.4 2,372,009 + CCNA_02223 0.16 -1.7 2,372,020 + CCNA_02223 0.17 -0.6 2,372,028 - CCNA_02223 0.18 +0.2 2,372,051 - CCNA_02223 0.20 -0.6 2,372,063 + CCNA_02223 0.22 -1.1 2,372,071 - CCNA_02223 0.23 +0.2 2,372,078 + CCNA_02223 0.24 -0.1 2,372,117 - CCNA_02223 0.28 +0.8 2,372,123 + CCNA_02223 0.29 +1.2 2,372,177 + CCNA_02223 0.35 +0.5 2,372,177 + CCNA_02223 0.35 -0.8 2,372,210 - CCNA_02223 0.39 +0.9 2,372,211 - CCNA_02223 0.39 +3.2 2,372,225 + CCNA_02223 0.40 -0.7 2,372,276 - CCNA_02223 0.46 +0.0 2,372,276 - CCNA_02223 0.46 -0.1 2,372,276 - CCNA_02223 0.46 +0.9 2,372,276 - CCNA_02223 0.46 +0.5 2,372,276 - CCNA_02223 0.46 +0.5 2,372,276 - CCNA_02223 0.46 +0.7 2,372,281 - CCNA_02223 0.47 -0.1 2,372,281 - CCNA_02223 0.47 +1.3 2,372,296 - CCNA_02223 0.49 +0.2 2,372,390 + CCNA_02223 0.59 -0.8 2,372,390 + CCNA_02223 0.59 -0.6 2,372,405 - CCNA_02223 0.61 -0.6 2,372,407 + CCNA_02223 0.61 -1.9 2,372,414 + CCNA_02223 0.62 -0.6 2,372,414 + CCNA_02223 0.62 -1.4 2,372,422 - CCNA_02223 0.63 +1.7 2,372,422 - CCNA_02223 0.63 +0.8 2,372,422 - CCNA_02223 0.63 +0.8 2,372,422 - CCNA_02223 0.63 +0.5 2,372,472 - CCNA_02223 0.69 +0.4 2,372,501 + CCNA_02223 0.72 -1.1 2,372,502 - CCNA_02223 0.72 +0.1 2,372,559 - CCNA_02223 0.79 -1.0 2,372,564 - CCNA_02223 0.79 +0.2 2,372,606 + CCNA_02223 0.84 -1.6 2,372,615 + CCNA_02223 0.85 -1.4 2,372,636 + CCNA_02223 0.88 +0.8 2,372,636 + CCNA_02223 0.88 -1.8 2,372,644 - CCNA_02223 0.89 -0.4 2,372,690 + -0.7 2,372,735 - +0.4 2,372,750 + -1.1 2,372,750 + -0.2 2,372,771 - +0.7 2,372,780 + -0.2 2,372,781 + -0.6 2,372,786 + -0.4 2,372,794 - -2.1 2,372,820 + -1.0 2,372,827 - +0.5 2,372,833 + -1.6 2,372,853 - CCNA_02224 0.12 +1.1 2,372,991 + CCNA_02224 0.27 -0.2 2,373,024 - CCNA_02224 0.30 +0.8 2,373,097 + CCNA_02224 0.38 -2.5 2,373,196 + CCNA_02224 0.48 +0.2 2,373,199 - CCNA_02224 0.49 +0.8 2,373,199 - CCNA_02224 0.49 +0.8 2,373,202 + CCNA_02224 0.49 +0.8 2,373,202 + CCNA_02224 0.49 +0.5 2,373,210 - CCNA_02224 0.50 +0.8 2,373,214 + CCNA_02224 0.50 -1.8 2,373,224 + CCNA_02224 0.51 +0.2 2,373,241 + CCNA_02224 0.53 -1.1 2,373,241 + CCNA_02224 0.53 +0.8 2,373,249 - CCNA_02224 0.54 +0.5 2,373,249 - CCNA_02224 0.54 +0.6 2,373,254 - CCNA_02224 0.54 +0.7 2,373,272 - CCNA_02224 0.56 +2.6 2,373,273 - CCNA_02224 0.57 +0.4 2,373,284 + CCNA_02224 0.58 -1.7 2,373,414 + CCNA_02224 0.71 -0.2 2,373,414 + CCNA_02224 0.71 -1.2 2,373,422 - CCNA_02224 0.72 +0.1 2,373,422 - CCNA_02224 0.72 -0.5 2,373,439 - CCNA_02224 0.74 +0.4 2,373,450 - CCNA_02224 0.75 +0.8 2,373,500 + CCNA_02224 0.81 -0.2 2,373,500 + CCNA_02224 0.81 +0.2 2,373,508 - CCNA_02224 0.81 -0.4 2,373,508 - CCNA_02224 0.81 +0.3 2,373,508 - CCNA_02224 0.81 -0.7 2,373,508 - CCNA_02224 0.81 +0.6 2,373,510 - CCNA_02224 0.82 +0.4 2,373,539 + CCNA_02224 0.85 +0.3 2,373,543 + CCNA_02224 0.85 -0.2 2,373,575 - CCNA_02224 0.88 -0.5 2,373,583 + CCNA_02224 0.89 -1.2 2,373,583 + CCNA_02224 0.89 -0.2 2,373,583 + CCNA_02224 0.89 -0.6 2,373,591 - +1.1 2,373,667 - +1.0 2,373,671 + -0.8 2,373,692 + -0.2 2,373,694 + +0.4 2,373,700 - +0.5 2,373,700 - +0.9 2,373,734 - +1.1 2,373,734 - +0.9 2,373,787 + CCNA_02225 0.11 -1.6 2,373,818 + CCNA_02225 0.14 -0.2 2,373,856 - CCNA_02225 0.18 +1.9 2,373,944 - CCNA_02225 0.27 +0.5 2,373,971 + CCNA_02225 0.30 -0.2 2,373,995 - CCNA_02225 0.32 +0.2 2,374,100 - CCNA_02225 0.43 +3.6 2,374,122 - CCNA_02225 0.45 +1.1 2,374,249 + CCNA_02225 0.58 +0.2 2,374,257 - CCNA_02225 0.59 +1.2 2,374,299 + CCNA_02225 0.63 +1.8 2,374,307 - CCNA_02225 0.64 +1.0 2,374,317 + CCNA_02225 0.65 -1.2 2,374,324 - CCNA_02225 0.66 +1.8 2,374,353 + CCNA_02225 0.69 +0.8 2,374,458 - CCNA_02225 0.80 +0.9 2,374,458 - CCNA_02225 0.80 +1.4 2,374,458 - CCNA_02225 0.80 +0.9 2,374,458 - CCNA_02225 0.80 +1.6 2,374,499 - CCNA_02225 0.84 +1.9 2,374,539 - CCNA_02225 0.88 +1.2 2,374,558 - CCNA_02225 0.90 +1.2
Or see this region's nucleotide sequence