Experiment: PYE with Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00069 and CCNA_00070 are separated by 72 nucleotides CCNA_00070 and CCNA_00071 are separated by 4 nucleotides CCNA_00071 and CCNA_00072 are separated by 43 nucleotides
CCNA_00069: CCNA_00069 - O-sialoglycoprotein endopeptidase, at 70,801 to 71,904
_00069
CCNA_00070: CCNA_00070 - porphobilinogen deaminase, at 71,977 to 72,945
_00070
CCNA_00071: CCNA_00071 - uroporphyrinogen-III synthase, at 72,950 to 73,636
_00071
CCNA_00072: CCNA_00072 - hypothetical protein, at 73,680 to 74,576
_00072
Position (kb)
71
72
73 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 71.967 kb on + strand at 71.975 kb on - strand at 72.967 kb on + strand at 72.967 kb on + strand at 72.967 kb on + strand at 73.615 kb on + strand at 73.623 kb on - strand at 73.632 kb on - strand at 73.650 kb on - strand at 73.650 kb on - strand at 73.667 kb on - strand at 73.723 kb on + strand at 73.731 kb on + strand at 73.739 kb on - strand at 73.776 kb on + strand, within CCNA_00072 at 73.786 kb on + strand, within CCNA_00072 at 73.800 kb on + strand, within CCNA_00072 at 73.822 kb on + strand, within CCNA_00072 at 73.830 kb on - strand, within CCNA_00072 at 73.830 kb on - strand, within CCNA_00072 at 73.830 kb on - strand, within CCNA_00072 at 73.830 kb on - strand, within CCNA_00072 at 73.831 kb on - strand, within CCNA_00072 at 73.890 kb on - strand, within CCNA_00072
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Sisomicin 0.004 mg/ml remove 71,967 + -1.0 71,975 - -0.7 72,967 + +1.7 72,967 + -0.1 72,967 + -1.7 73,615 + -0.0 73,623 - -1.0 73,632 - -0.6 73,650 - -0.2 73,650 - +0.0 73,667 - +1.1 73,723 + +1.8 73,731 + +0.8 73,739 - +1.0 73,776 + CCNA_00072 0.11 +2.1 73,786 + CCNA_00072 0.12 +1.5 73,800 + CCNA_00072 0.13 +1.7 73,822 + CCNA_00072 0.16 +1.7 73,830 - CCNA_00072 0.17 +1.0 73,830 - CCNA_00072 0.17 +1.8 73,830 - CCNA_00072 0.17 +0.7 73,830 - CCNA_00072 0.17 +4.2 73,831 - CCNA_00072 0.17 -0.7 73,890 - CCNA_00072 0.23 +0.8
Or see this region's nucleotide sequence