Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS14845 and AZOBR_RS14850 overlap by 4 nucleotides AZOBR_RS14850 and AZOBR_RS14855 are separated by 23 nucleotides AZOBR_RS14855 and AZOBR_RS14860 are separated by 16 nucleotides
AZOBR_RS14845: AZOBR_RS14845 - cysteine desulfurase, at 103,768 to 105,030
_RS14845
AZOBR_RS14850: AZOBR_RS14850 - nitrogen fixation protein NifU, at 105,027 to 105,494
_RS14850
AZOBR_RS14855: AZOBR_RS14855 - hypothetical protein, at 105,518 to 105,925
_RS14855
AZOBR_RS14860: AZOBR_RS14860 - hypothetical protein, at 105,942 to 106,298
_RS14860
Position (kb) 105
106
107 Strain fitness (log2 ratio) -2
-1
0
1
2 at 105.601 kb on + strand, within AZOBR_RS14855 at 105.665 kb on + strand, within AZOBR_RS14855 at 105.665 kb on + strand, within AZOBR_RS14855 at 105.710 kb on + strand, within AZOBR_RS14855 at 105.710 kb on + strand, within AZOBR_RS14855 at 105.711 kb on - strand, within AZOBR_RS14855 at 105.916 kb on + strand at 106.042 kb on - strand, within AZOBR_RS14860 at 106.422 kb on - strand at 106.514 kb on + strand at 106.830 kb on - strand at 106.972 kb on + strand at 106.973 kb on - strand at 106.976 kb on + strand at 106.976 kb on + strand at 107.114 kb on - strand at 107.116 kb on - strand at 107.173 kb on - strand at 107.183 kb on + strand at 107.275 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 105,601 + AZOBR_RS14855 0.20 +2.6 105,665 + AZOBR_RS14855 0.36 -2.6 105,665 + AZOBR_RS14855 0.36 +0.7 105,710 + AZOBR_RS14855 0.47 +1.6 105,710 + AZOBR_RS14855 0.47 +2.5 105,711 - AZOBR_RS14855 0.47 +2.0 105,916 + +1.9 106,042 - AZOBR_RS14860 0.28 +2.0 106,422 - +0.6 106,514 + +1.9 106,830 - -0.8 106,972 + -1.9 106,973 - +0.0 106,976 + -0.4 106,976 + -0.0 107,114 - -0.7 107,116 - +2.0 107,173 - -0.7 107,183 + +0.0 107,275 + +0.1
Or see this region's nucleotide sequence