Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS02305 and AZOBR_RS02310 are separated by 151 nucleotides AZOBR_RS02310 and AZOBR_RS02315 are separated by 4 nucleotides AZOBR_RS02315 and AZOBR_RS02320 are separated by 168 nucleotides
AZOBR_RS02305: AZOBR_RS02305 - cell envelope biogenesis protein LolA, at 476,075 to 476,737
_RS02305
AZOBR_RS02310: AZOBR_RS02310 - membrane protein, at 476,889 to 477,509
_RS02310
AZOBR_RS02315: AZOBR_RS02315 - ATPase AAA, at 477,514 to 479,046
_RS02315
AZOBR_RS02320: AZOBR_RS02320 - amidase, at 479,215 to 479,961
_RS02320
Position (kb)
477
478
479
480 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 476.721 kb on - strand at 476.894 kb on + strand at 476.894 kb on + strand at 476.908 kb on - strand at 476.908 kb on - strand at 477.061 kb on - strand, within AZOBR_RS02310 at 477.400 kb on - strand, within AZOBR_RS02310 at 477.507 kb on + strand at 477.516 kb on - strand at 477.783 kb on - strand, within AZOBR_RS02315 at 479.083 kb on - strand at 479.254 kb on + strand at 479.254 kb on + strand at 479.255 kb on - strand at 479.488 kb on + strand, within AZOBR_RS02320 at 479.488 kb on + strand, within AZOBR_RS02320 at 479.488 kb on + strand, within AZOBR_RS02320 at 479.488 kb on + strand, within AZOBR_RS02320 at 479.489 kb on - strand, within AZOBR_RS02320 at 479.489 kb on - strand, within AZOBR_RS02320 at 479.489 kb on - strand, within AZOBR_RS02320 at 479.578 kb on + strand, within AZOBR_RS02320 at 479.579 kb on - strand, within AZOBR_RS02320 at 479.587 kb on + strand, within AZOBR_RS02320 at 479.746 kb on + strand, within AZOBR_RS02320 at 479.746 kb on + strand, within AZOBR_RS02320 at 479.746 kb on + strand, within AZOBR_RS02320 at 479.747 kb on - strand, within AZOBR_RS02320 at 479.747 kb on - strand, within AZOBR_RS02320 at 479.747 kb on - strand, within AZOBR_RS02320 at 479.812 kb on + strand, within AZOBR_RS02320 at 479.813 kb on - strand, within AZOBR_RS02320 at 479.935 kb on + strand at 479.936 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 476,721 - +1.0 476,894 + +0.5 476,894 + +1.1 476,908 - +0.4 476,908 - -0.3 477,061 - AZOBR_RS02310 0.28 +0.4 477,400 - AZOBR_RS02310 0.82 -1.5 477,507 + -0.8 477,516 - +1.3 477,783 - AZOBR_RS02315 0.18 -0.7 479,083 - -4.0 479,254 + -0.0 479,254 + +0.6 479,255 - -0.1 479,488 + AZOBR_RS02320 0.37 -3.9 479,488 + AZOBR_RS02320 0.37 +0.2 479,488 + AZOBR_RS02320 0.37 +0.5 479,488 + AZOBR_RS02320 0.37 +0.2 479,489 - AZOBR_RS02320 0.37 -0.2 479,489 - AZOBR_RS02320 0.37 -1.0 479,489 - AZOBR_RS02320 0.37 -0.6 479,578 + AZOBR_RS02320 0.49 +0.9 479,579 - AZOBR_RS02320 0.49 -0.3 479,587 + AZOBR_RS02320 0.50 +0.7 479,746 + AZOBR_RS02320 0.71 -2.1 479,746 + AZOBR_RS02320 0.71 -1.8 479,746 + AZOBR_RS02320 0.71 +0.4 479,747 - AZOBR_RS02320 0.71 +4.2 479,747 - AZOBR_RS02320 0.71 -1.2 479,747 - AZOBR_RS02320 0.71 -2.4 479,812 + AZOBR_RS02320 0.80 +0.2 479,813 - AZOBR_RS02320 0.80 +0.3 479,935 + +0.5 479,936 - +0.9
Or see this region's nucleotide sequence