Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01300 and AZOBR_RS01305 are separated by 148 nucleotides AZOBR_RS01305 and AZOBR_RS01310 are separated by 93 nucleotides AZOBR_RS01310 and AZOBR_RS01315 are separated by 299 nucleotides
AZOBR_RS01300: AZOBR_RS01300 - peptide ABC transporter substrate-binding protein, at 269,287 to 271,095
_RS01300
AZOBR_RS01305: AZOBR_RS01305 - inorganic pyrophosphatase, at 271,244 to 271,801
_RS01305
AZOBR_RS01310: AZOBR_RS01310 - lactate dehydrogenase, at 271,895 to 272,836
_RS01310
AZOBR_RS01315: AZOBR_RS01315 - GCN5 family N-acetyltransferase, at 273,136 to 275,820
_RS01315
Position (kb) 271
272
273 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 270.983 kb on + strand at 271.142 kb on + strand at 272.184 kb on + strand, within AZOBR_RS01310 at 272.960 kb on + strand at 272.981 kb on + strand at 273.041 kb on - strand at 273.310 kb on + strand at 273.310 kb on + strand at 273.311 kb on - strand at 273.311 kb on - strand at 273.718 kb on + strand, within AZOBR_RS01315 at 273.719 kb on - strand, within AZOBR_RS01315 at 273.719 kb on - strand at 273.719 kb on - strand, within AZOBR_RS01315 at 273.757 kb on + strand, within AZOBR_RS01315 at 273.758 kb on - strand, within AZOBR_RS01315
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 270,983 + +1.3 271,142 + +0.1 272,184 + AZOBR_RS01310 0.31 +0.3 272,960 + +0.6 272,981 + -0.2 273,041 - -3.1 273,310 + +2.2 273,310 + +1.3 273,311 - -1.3 273,311 - -3.3 273,718 + AZOBR_RS01315 0.22 -3.4 273,719 - AZOBR_RS01315 0.22 -1.1 273,719 - -1.3 273,719 - AZOBR_RS01315 0.22 -0.5 273,757 + AZOBR_RS01315 0.23 +0.3 273,758 - AZOBR_RS01315 0.23 +0.6
Or see this region's nucleotide sequence