Experiment: soil EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS16720 and ABZR87_RS16725 are separated by 162 nucleotides ABZR87_RS16725 and ABZR87_RS16730 are separated by 82 nucleotides
ABZR87_RS16720: ABZR87_RS16720 - collagen-like triple helix repeat-containing protein, at 100,155 to 101,300
_RS16720
ABZR87_RS16725: ABZR87_RS16725 - antibiotic biosynthesis monooxygenase, at 101,463 to 101,771
_RS16725
ABZR87_RS16730: ABZR87_RS16730 - 2-methylaconitate cis-trans isomerase PrpF, at 101,854 to 103,038
_RS16730
Position (kb) 101
102 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 100.535 kb on + strand, within ABZR87_RS16720 at 100.913 kb on + strand, within ABZR87_RS16720 at 100.914 kb on - strand, within ABZR87_RS16720 at 101.377 kb on + strand at 101.377 kb on + strand at 101.377 kb on + strand at 101.389 kb on + strand at 101.389 kb on + strand at 101.389 kb on + strand at 101.390 kb on - strand at 101.415 kb on + strand at 101.415 kb on + strand at 101.415 kb on + strand at 101.415 kb on + strand at 101.416 kb on - strand at 101.416 kb on - strand at 101.416 kb on - strand at 101.416 kb on - strand at 101.416 kb on - strand at 101.416 kb on - strand at 101.420 kb on + strand at 101.420 kb on + strand at 101.421 kb on - strand at 101.421 kb on - strand at 102.173 kb on + strand, within ABZR87_RS16730 at 102.173 kb on + strand, within ABZR87_RS16730 at 102.173 kb on + strand, within ABZR87_RS16730 at 102.174 kb on - strand, within ABZR87_RS16730 at 102.174 kb on - strand, within ABZR87_RS16730 at 102.174 kb on - strand, within ABZR87_RS16730 at 102.345 kb on - strand, within ABZR87_RS16730
Per-strain Table
Position Strand Gene LocusTag Fraction soil EcoFAB sample 2; outgrowth in LB remove 100,535 + ABZR87_RS16720 0.33 -1.9 100,913 + ABZR87_RS16720 0.66 +0.8 100,914 - ABZR87_RS16720 0.66 +0.1 101,377 + +0.8 101,377 + +0.8 101,377 + +0.8 101,389 + +0.8 101,389 + -0.1 101,389 + -0.6 101,390 - +0.9 101,415 + +0.8 101,415 + +0.6 101,415 + -0.0 101,415 + +0.2 101,416 - -0.0 101,416 - -0.6 101,416 - -0.1 101,416 - +0.6 101,416 - -0.0 101,416 - +0.3 101,420 + +1.2 101,420 + +0.2 101,421 - -1.2 101,421 - +0.1 102,173 + ABZR87_RS16730 0.27 +1.2 102,173 + ABZR87_RS16730 0.27 -3.1 102,173 + ABZR87_RS16730 0.27 -1.8 102,174 - ABZR87_RS16730 0.27 -2.9 102,174 - ABZR87_RS16730 0.27 -3.2 102,174 - ABZR87_RS16730 0.27 -4.3 102,345 - ABZR87_RS16730 0.41 -2.7
Or see this region's nucleotide sequence