Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS09420

Experiment: soil EcoFAB sample 2; outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS09410 and ABZR87_RS09415 are separated by 118 nucleotidesABZR87_RS09415 and ABZR87_RS09420 overlap by 4 nucleotidesABZR87_RS09420 and ABZR87_RS09425 are separated by 159 nucleotidesABZR87_RS09425 and ABZR87_RS09430 are separated by 176 nucleotides ABZR87_RS09410: ABZR87_RS09410 - DUF748 domain-containing protein, at 1,995,194 to 1,998,976 _RS09410 ABZR87_RS09415: ABZR87_RS09415 - HAD-IA family hydrolase, at 1,999,095 to 1,999,784 _RS09415 ABZR87_RS09420: ABZR87_RS09420 - bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG, at 1,999,781 to 2,000,497 _RS09420 ABZR87_RS09425: ABZR87_RS09425 - glycine zipper 2TM domain-containing protein, at 2,000,657 to 2,001,166 _RS09425 ABZR87_RS09430: ABZR87_RS09430 - outer membrane protein OmpA, at 2,001,343 to 2,001,999 _RS09430 Position (kb) 1999 2000 2001Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1998.862 kb on + strandat 1998.862 kb on + strandat 1998.863 kb on - strandat 1998.863 kb on - strandat 1998.896 kb on - strandat 1998.896 kb on - strandat 1998.934 kb on + strandat 1998.934 kb on + strandat 1999.006 kb on + strandat 1999.007 kb on - strandat 1999.195 kb on + strand, within ABZR87_RS09415at 1999.201 kb on + strand, within ABZR87_RS09415at 1999.201 kb on + strand, within ABZR87_RS09415at 1999.201 kb on + strand, within ABZR87_RS09415at 1999.217 kb on + strand, within ABZR87_RS09415at 1999.217 kb on + strand, within ABZR87_RS09415at 1999.218 kb on - strand, within ABZR87_RS09415at 1999.218 kb on - strand, within ABZR87_RS09415at 1999.218 kb on - strand, within ABZR87_RS09415at 1999.270 kb on + strand, within ABZR87_RS09415at 1999.270 kb on + strand, within ABZR87_RS09415at 1999.531 kb on + strand, within ABZR87_RS09415at 1999.532 kb on - strand, within ABZR87_RS09415at 1999.532 kb on - strand, within ABZR87_RS09415at 1999.532 kb on - strand, within ABZR87_RS09415at 1999.648 kb on + strand, within ABZR87_RS09415at 1999.648 kb on + strand, within ABZR87_RS09415at 1999.649 kb on - strand, within ABZR87_RS09415at 1999.649 kb on - strand, within ABZR87_RS09415at 1999.649 kb on - strand, within ABZR87_RS09415at 2000.525 kb on - strandat 2000.525 kb on - strandat 2000.525 kb on - strandat 2000.525 kb on - strandat 2000.660 kb on - strandat 2000.679 kb on + strandat 2000.679 kb on + strandat 2000.679 kb on + strandat 2000.744 kb on + strand, within ABZR87_RS09425at 2000.744 kb on + strand, within ABZR87_RS09425at 2000.744 kb on + strand, within ABZR87_RS09425at 2000.746 kb on + strand, within ABZR87_RS09425at 2000.777 kb on + strand, within ABZR87_RS09425at 2000.778 kb on - strand, within ABZR87_RS09425at 2000.795 kb on + strand, within ABZR87_RS09425at 2000.795 kb on + strand, within ABZR87_RS09425at 2000.796 kb on - strand, within ABZR87_RS09425at 2000.829 kb on - strand, within ABZR87_RS09425at 2000.829 kb on - strand, within ABZR87_RS09425at 2000.951 kb on - strand, within ABZR87_RS09425at 2000.990 kb on + strand, within ABZR87_RS09425at 2000.990 kb on + strand, within ABZR87_RS09425at 2000.990 kb on + strand, within ABZR87_RS09425at 2000.991 kb on - strand, within ABZR87_RS09425at 2000.991 kb on - strand, within ABZR87_RS09425at 2001.158 kb on + strandat 2001.158 kb on + strandat 2001.158 kb on + strandat 2001.159 kb on - strandat 2001.159 kb on - strandat 2001.159 kb on - strandat 2001.159 kb on - strandat 2001.159 kb on - strandat 2001.164 kb on + strandat 2001.164 kb on + strandat 2001.165 kb on - strandat 2001.165 kb on - strandat 2001.177 kb on + strandat 2001.245 kb on + strandat 2001.290 kb on + strandat 2001.290 kb on + strandat 2001.290 kb on + strandat 2001.291 kb on - strandat 2001.291 kb on - strandat 2001.291 kb on - strandat 2001.292 kb on + strandat 2001.293 kb on - strandat 2001.293 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction soil EcoFAB sample 2; outgrowth in LB
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1,998,862 + +0.2
1,998,862 + -1.7
1,998,863 - +1.0
1,998,863 - +0.1
1,998,896 - -0.9
1,998,896 - +0.1
1,998,934 + -1.8
1,998,934 + +0.1
1,999,006 + -0.6
1,999,007 - -0.5
1,999,195 + ABZR87_RS09415 0.14 -3.5
1,999,201 + ABZR87_RS09415 0.15 -0.7
1,999,201 + ABZR87_RS09415 0.15 +0.2
1,999,201 + ABZR87_RS09415 0.15 -1.7
1,999,217 + ABZR87_RS09415 0.18 +0.1
1,999,217 + ABZR87_RS09415 0.18 -1.8
1,999,218 - ABZR87_RS09415 0.18 -0.8
1,999,218 - ABZR87_RS09415 0.18 -1.1
1,999,218 - ABZR87_RS09415 0.18 +1.1
1,999,270 + ABZR87_RS09415 0.25 -3.3
1,999,270 + ABZR87_RS09415 0.25 -0.4
1,999,531 + ABZR87_RS09415 0.63 +1.5
1,999,532 - ABZR87_RS09415 0.63 +0.7
1,999,532 - ABZR87_RS09415 0.63 -3.4
1,999,532 - ABZR87_RS09415 0.63 +2.3
1,999,648 + ABZR87_RS09415 0.80 -1.6
1,999,648 + ABZR87_RS09415 0.80 -0.4
1,999,649 - ABZR87_RS09415 0.80 +1.0
1,999,649 - ABZR87_RS09415 0.80 -2.4
1,999,649 - ABZR87_RS09415 0.80 -1.6
2,000,525 - +0.5
2,000,525 - +0.7
2,000,525 - +0.7
2,000,525 - +1.6
2,000,660 - -0.4
2,000,679 + +1.2
2,000,679 + +0.5
2,000,679 + +1.5
2,000,744 + ABZR87_RS09425 0.17 -0.9
2,000,744 + ABZR87_RS09425 0.17 +0.0
2,000,744 + ABZR87_RS09425 0.17 -0.4
2,000,746 + ABZR87_RS09425 0.17 +0.2
2,000,777 + ABZR87_RS09425 0.24 -1.1
2,000,778 - ABZR87_RS09425 0.24 +1.0
2,000,795 + ABZR87_RS09425 0.27 -1.4
2,000,795 + ABZR87_RS09425 0.27 +0.4
2,000,796 - ABZR87_RS09425 0.27 +1.1
2,000,829 - ABZR87_RS09425 0.34 +0.2
2,000,829 - ABZR87_RS09425 0.34 +0.5
2,000,951 - ABZR87_RS09425 0.58 -0.0
2,000,990 + ABZR87_RS09425 0.65 -0.7
2,000,990 + ABZR87_RS09425 0.65 -0.8
2,000,990 + ABZR87_RS09425 0.65 +1.2
2,000,991 - ABZR87_RS09425 0.65 +0.7
2,000,991 - ABZR87_RS09425 0.65 -1.5
2,001,158 + +1.5
2,001,158 + +0.3
2,001,158 + -1.8
2,001,159 - +1.1
2,001,159 - +3.3
2,001,159 - +0.5
2,001,159 - -0.9
2,001,159 - +0.8
2,001,164 + -0.4
2,001,164 + +1.6
2,001,165 - +0.7
2,001,165 - -0.2
2,001,177 + +0.2
2,001,245 + -1.4
2,001,290 + +0.9
2,001,290 + +1.3
2,001,290 + -0.3
2,001,291 - +0.3
2,001,291 - +0.8
2,001,291 - -1.1
2,001,292 + +1.5
2,001,293 - -0.7
2,001,293 - -1.5

Or see this region's nucleotide sequence