Experiment: soil EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS01745 and ABZR87_RS01750 overlap by 8 nucleotides ABZR87_RS01750 and ABZR87_RS01755 are separated by 39 nucleotides
ABZR87_RS01745: ABZR87_RS01745 - 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD, at 371,449 to 372,045
_RS01745
ABZR87_RS01750: ABZR87_RS01750 - sigma-54 dependent transcriptional regulator, at 372,038 to 373,663
_RS01750
ABZR87_RS01755: ABZR87_RS01755 - ATP-binding protein, at 373,703 to 375,742
_RS01755
Position (kb)
372
373
374 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 371.063 kb on + strand at 371.064 kb on - strand at 371.275 kb on - strand at 371.275 kb on - strand at 371.316 kb on + strand at 371.465 kb on + strand at 371.588 kb on + strand, within ABZR87_RS01745 at 371.630 kb on + strand, within ABZR87_RS01745 at 371.631 kb on - strand, within ABZR87_RS01745 at 371.631 kb on - strand, within ABZR87_RS01745 at 371.631 kb on - strand, within ABZR87_RS01745 at 371.631 kb on - strand, within ABZR87_RS01745 at 371.831 kb on + strand, within ABZR87_RS01745 at 371.831 kb on + strand, within ABZR87_RS01745 at 371.831 kb on + strand, within ABZR87_RS01745 at 371.832 kb on - strand, within ABZR87_RS01745 at 371.832 kb on - strand, within ABZR87_RS01745 at 372.191 kb on - strand at 372.247 kb on - strand, within ABZR87_RS01750 at 372.247 kb on - strand, within ABZR87_RS01750 at 372.373 kb on + strand, within ABZR87_RS01750 at 372.374 kb on - strand, within ABZR87_RS01750 at 372.374 kb on - strand, within ABZR87_RS01750 at 372.374 kb on - strand, within ABZR87_RS01750 at 372.374 kb on - strand, within ABZR87_RS01750 at 372.375 kb on + strand, within ABZR87_RS01750 at 372.375 kb on + strand, within ABZR87_RS01750 at 372.375 kb on + strand, within ABZR87_RS01750 at 372.375 kb on + strand, within ABZR87_RS01750 at 372.375 kb on + strand, within ABZR87_RS01750 at 372.375 kb on + strand, within ABZR87_RS01750 at 372.375 kb on + strand, within ABZR87_RS01750 at 372.376 kb on - strand, within ABZR87_RS01750 at 372.376 kb on - strand, within ABZR87_RS01750 at 372.376 kb on - strand, within ABZR87_RS01750 at 372.376 kb on - strand, within ABZR87_RS01750 at 372.376 kb on - strand, within ABZR87_RS01750 at 372.750 kb on + strand, within ABZR87_RS01750 at 372.750 kb on + strand, within ABZR87_RS01750 at 372.751 kb on - strand, within ABZR87_RS01750 at 373.011 kb on + strand, within ABZR87_RS01750 at 373.347 kb on + strand, within ABZR87_RS01750 at 373.347 kb on + strand, within ABZR87_RS01750 at 373.348 kb on - strand, within ABZR87_RS01750 at 373.704 kb on + strand at 373.705 kb on - strand at 373.861 kb on + strand at 373.861 kb on + strand at 373.881 kb on + strand at 373.881 kb on + strand at 373.881 kb on + strand at 373.881 kb on + strand at 373.881 kb on + strand at 373.882 kb on - strand at 373.882 kb on - strand at 373.882 kb on - strand at 373.882 kb on - strand at 373.882 kb on - strand at 373.882 kb on - strand at 373.882 kb on - strand at 373.951 kb on + strand, within ABZR87_RS01755 at 374.010 kb on + strand, within ABZR87_RS01755 at 374.011 kb on - strand, within ABZR87_RS01755 at 374.049 kb on + strand, within ABZR87_RS01755 at 374.346 kb on - strand, within ABZR87_RS01755
Per-strain Table
Position Strand Gene LocusTag Fraction soil EcoFAB sample 2; outgrowth in LB remove 371,063 + -0.2 371,064 - +1.4 371,275 - +0.9 371,275 - -0.7 371,316 + -0.1 371,465 + -1.2 371,588 + ABZR87_RS01745 0.23 -3.6 371,630 + ABZR87_RS01745 0.30 -1.6 371,631 - ABZR87_RS01745 0.30 -3.6 371,631 - ABZR87_RS01745 0.30 -2.0 371,631 - ABZR87_RS01745 0.30 -3.3 371,631 - ABZR87_RS01745 0.30 -2.6 371,831 + ABZR87_RS01745 0.64 -0.9 371,831 + ABZR87_RS01745 0.64 -2.8 371,831 + ABZR87_RS01745 0.64 -0.9 371,832 - ABZR87_RS01745 0.64 -0.6 371,832 - ABZR87_RS01745 0.64 -2.8 372,191 - +1.4 372,247 - ABZR87_RS01750 0.13 +1.4 372,247 - ABZR87_RS01750 0.13 +1.7 372,373 + ABZR87_RS01750 0.21 +1.9 372,374 - ABZR87_RS01750 0.21 +3.4 372,374 - ABZR87_RS01750 0.21 +2.5 372,374 - ABZR87_RS01750 0.21 +0.8 372,374 - ABZR87_RS01750 0.21 +1.6 372,375 + ABZR87_RS01750 0.21 +1.7 372,375 + ABZR87_RS01750 0.21 +1.7 372,375 + ABZR87_RS01750 0.21 +1.3 372,375 + ABZR87_RS01750 0.21 +1.3 372,375 + ABZR87_RS01750 0.21 +1.8 372,375 + ABZR87_RS01750 0.21 +1.5 372,375 + ABZR87_RS01750 0.21 +0.5 372,376 - ABZR87_RS01750 0.21 +2.0 372,376 - ABZR87_RS01750 0.21 +0.0 372,376 - ABZR87_RS01750 0.21 -0.2 372,376 - ABZR87_RS01750 0.21 +1.7 372,376 - ABZR87_RS01750 0.21 +1.5 372,750 + ABZR87_RS01750 0.44 +1.1 372,750 + ABZR87_RS01750 0.44 +0.7 372,751 - ABZR87_RS01750 0.44 +1.9 373,011 + ABZR87_RS01750 0.60 -0.8 373,347 + ABZR87_RS01750 0.81 +1.7 373,347 + ABZR87_RS01750 0.81 +0.2 373,348 - ABZR87_RS01750 0.81 +1.2 373,704 + +0.8 373,705 - -0.4 373,861 + +0.6 373,861 + +2.8 373,881 + +0.1 373,881 + +2.1 373,881 + +2.1 373,881 + +3.0 373,881 + +2.1 373,882 - -1.3 373,882 - -1.1 373,882 - -0.1 373,882 - +0.8 373,882 - -0.1 373,882 - -0.3 373,882 - -0.5 373,951 + ABZR87_RS01755 0.12 +1.7 374,010 + ABZR87_RS01755 0.15 +1.8 374,011 - ABZR87_RS01755 0.15 +1.3 374,049 + ABZR87_RS01755 0.17 +1.8 374,346 - ABZR87_RS01755 0.32 +0.4
Or see this region's nucleotide sequence