Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2954 and Shew_2955 are separated by 261 nucleotides Shew_2955 and Shew_2956 are separated by 271 nucleotides Shew_2956 and Shew_2957 overlap by 1 nucleotides
Shew_2954: Shew_2954 - methyl-accepting chemotaxis sensory transducer (RefSeq), at 3,501,394 to 3,503,256
_2954
Shew_2955: Shew_2955 - ornithine cyclodeaminase (RefSeq), at 3,503,518 to 3,504,462
_2955
Shew_2956: Shew_2956 - ECF subfamily RNA polymerase sigma-24 factor (RefSeq), at 3,504,734 to 3,505,306
_2956
Shew_2957: Shew_2957 - hypothetical protein (RefSeq), at 3,505,306 to 3,505,818
_2957
Position (kb)
3503
3504
3505 Strain fitness (log2 ratio)
-1
0
1
2 at 3502.854 kb on + strand, within Shew_2954 at 3502.947 kb on + strand, within Shew_2954 at 3503.035 kb on - strand, within Shew_2954 at 3503.039 kb on + strand, within Shew_2954 at 3503.067 kb on - strand, within Shew_2954 at 3503.131 kb on + strand at 3503.220 kb on - strand at 3503.599 kb on - strand at 3503.700 kb on + strand, within Shew_2955 at 3504.033 kb on - strand, within Shew_2955 at 3504.282 kb on + strand, within Shew_2955 at 3504.493 kb on + strand at 3504.550 kb on - strand at 3505.053 kb on + strand, within Shew_2956 at 3505.182 kb on + strand, within Shew_2956 at 3505.270 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 3,502,854 + Shew_2954 0.78 -0.6 3,502,947 + Shew_2954 0.83 +0.3 3,503,035 - Shew_2954 0.88 -0.4 3,503,039 + Shew_2954 0.88 -0.4 3,503,067 - Shew_2954 0.90 +0.0 3,503,131 + +2.3 3,503,220 - -0.0 3,503,599 - +0.1 3,503,700 + Shew_2955 0.19 -0.3 3,504,033 - Shew_2955 0.54 -1.5 3,504,282 + Shew_2955 0.81 -0.2 3,504,493 + -0.1 3,504,550 - +0.2 3,505,053 + Shew_2956 0.56 -0.2 3,505,182 + Shew_2956 0.78 -0.1 3,505,270 - +0.1
Or see this region's nucleotide sequence