Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2652 and Shew_2653 are separated by 44 nucleotides Shew_2653 and queF are separated by 27 nucleotides queF and Shew_2655 are separated by 322 nucleotides
Shew_2652: Shew_2652 - hypothetical protein (RefSeq), at 3,125,582 to 3,126,355
_2652
Shew_2653: Shew_2653 - SecY interacting protein Syd (RefSeq), at 3,126,400 to 3,127,062
_2653
Shew_2654: queF - 7-cyano-7-deazaguanine reductase (RefSeq), at 3,127,090 to 3,127,980
queF
Shew_2655: Shew_2655 - hypothetical protein (RefSeq), at 3,128,303 to 3,129,493
_2655
Position (kb)
3127
3128 Strain fitness (log2 ratio)
-1
0
1 at 3126.246 kb on - strand, within Shew_2652 at 3126.875 kb on - strand, within Shew_2653 at 3127.427 kb on + strand, within queF at 3127.676 kb on + strand, within queF at 3127.676 kb on + strand, within queF at 3127.684 kb on - strand, within queF at 3128.051 kb on + strand at 3128.059 kb on - strand at 3128.059 kb on - strand at 3128.069 kb on - strand at 3128.071 kb on - strand at 3128.442 kb on + strand, within Shew_2655 at 3128.500 kb on - strand, within Shew_2655 at 3128.636 kb on + strand, within Shew_2655 at 3128.643 kb on - strand, within Shew_2655 at 3128.752 kb on - strand, within Shew_2655 at 3128.754 kb on - strand, within Shew_2655 at 3128.960 kb on - strand, within Shew_2655
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 3,126,246 - Shew_2652 0.86 +0.8 3,126,875 - Shew_2653 0.72 +0.1 3,127,427 + queF Shew_2654 0.38 -0.7 3,127,676 + queF Shew_2654 0.66 +0.2 3,127,676 + queF Shew_2654 0.66 -1.2 3,127,684 - queF Shew_2654 0.67 +0.1 3,128,051 + +0.2 3,128,059 - +0.0 3,128,059 - +0.1 3,128,069 - -0.4 3,128,071 - -0.3 3,128,442 + Shew_2655 0.12 -0.2 3,128,500 - Shew_2655 0.17 +0.1 3,128,636 + Shew_2655 0.28 +0.4 3,128,643 - Shew_2655 0.29 +0.1 3,128,752 - Shew_2655 0.38 -0.1 3,128,754 - Shew_2655 0.38 +0.8 3,128,960 - Shew_2655 0.55 -0.3
Or see this region's nucleotide sequence