Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2547 and Shew_2548 are separated by 7 nucleotides Shew_2548 and Shew_2549 are separated by 5 nucleotides Shew_2549 and Shew_2550 are separated by 230 nucleotides
Shew_2547: Shew_2547 - pseudouridine synthase (RefSeq), at 3,003,986 to 3,004,849
_2547
Shew_2548: Shew_2548 - potassium efflux system protein (RefSeq), at 3,004,857 to 3,006,845
_2548
Shew_2549: Shew_2549 - NAD(P)H dehydrogenase (quinone) (RefSeq), at 3,006,851 to 3,007,468
_2549
Shew_2550: Shew_2550 - GCN5-related N-acetyltransferase (RefSeq), at 3,007,699 to 3,008,244
_2550
Position (kb)
3004
3005
3006
3007 Strain fitness (log2 ratio)
-1
0
1 at 3004.002 kb on + strand at 3004.242 kb on - strand, within Shew_2547 at 3004.242 kb on - strand, within Shew_2547 at 3004.394 kb on + strand, within Shew_2547 at 3004.394 kb on + strand, within Shew_2547 at 3004.545 kb on + strand, within Shew_2547 at 3005.117 kb on + strand, within Shew_2548 at 3005.149 kb on - strand, within Shew_2548 at 3005.512 kb on + strand, within Shew_2548 at 3005.515 kb on - strand, within Shew_2548 at 3005.738 kb on + strand, within Shew_2548 at 3005.796 kb on - strand, within Shew_2548 at 3005.798 kb on + strand, within Shew_2548 at 3006.486 kb on + strand, within Shew_2548 at 3006.936 kb on + strand, within Shew_2549 at 3007.225 kb on + strand, within Shew_2549
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 3,004,002 + -0.4 3,004,242 - Shew_2547 0.30 -0.0 3,004,242 - Shew_2547 0.30 +0.4 3,004,394 + Shew_2547 0.47 +0.3 3,004,394 + Shew_2547 0.47 -0.3 3,004,545 + Shew_2547 0.65 +0.3 3,005,117 + Shew_2548 0.13 -0.4 3,005,149 - Shew_2548 0.15 -0.6 3,005,512 + Shew_2548 0.33 -0.3 3,005,515 - Shew_2548 0.33 -0.7 3,005,738 + Shew_2548 0.44 -0.4 3,005,796 - Shew_2548 0.47 -0.7 3,005,798 + Shew_2548 0.47 -0.6 3,006,486 + Shew_2548 0.82 -0.6 3,006,936 + Shew_2549 0.14 -0.4 3,007,225 + Shew_2549 0.61 -1.6
Or see this region's nucleotide sequence