Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1735 and Shew_1736 are separated by 64 nucleotides Shew_1736 and Shew_1737 are separated by 43 nucleotides Shew_1737 and Shew_1738 are separated by 39 nucleotides
Shew_1735: Shew_1735 - TonB-dependent receptor (RefSeq), at 1,985,370 to 1,987,511
_1735
Shew_1736: Shew_1736 - hypothetical protein (RefSeq), at 1,987,576 to 1,987,836
_1736
Shew_1737: Shew_1737 - nicotinamide mononucleotide transporter PnuC (RefSeq), at 1,987,880 to 1,988,527
_1737
Shew_1738: Shew_1738 - aminoglycoside phosphotransferase (RefSeq), at 1,988,567 to 1,989,706
_1738
Position (kb)
1987
1988 Strain fitness (log2 ratio)
-1
0
1 at 1986.770 kb on - strand, within Shew_1735 at 1986.865 kb on + strand, within Shew_1735 at 1986.865 kb on + strand, within Shew_1735 at 1987.312 kb on - strand at 1987.653 kb on + strand, within Shew_1736 at 1987.984 kb on - strand, within Shew_1737 at 1988.424 kb on + strand, within Shew_1737 at 1988.424 kb on + strand, within Shew_1737 at 1988.432 kb on - strand, within Shew_1737 at 1988.432 kb on - strand, within Shew_1737 at 1988.463 kb on + strand at 1988.747 kb on - strand, within Shew_1738 at 1988.771 kb on + strand, within Shew_1738 at 1988.802 kb on + strand, within Shew_1738 at 1988.810 kb on - strand, within Shew_1738
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 1,986,770 - Shew_1735 0.65 -0.1 1,986,865 + Shew_1735 0.70 +0.2 1,986,865 + Shew_1735 0.70 +0.0 1,987,312 - -0.0 1,987,653 + Shew_1736 0.30 -0.5 1,987,984 - Shew_1737 0.16 -0.4 1,988,424 + Shew_1737 0.84 -0.4 1,988,424 + Shew_1737 0.84 -0.4 1,988,432 - Shew_1737 0.85 +0.2 1,988,432 - Shew_1737 0.85 +0.1 1,988,463 + +0.1 1,988,747 - Shew_1738 0.16 +0.1 1,988,771 + Shew_1738 0.18 +0.4 1,988,802 + Shew_1738 0.21 -0.2 1,988,810 - Shew_1738 0.21 +0.5
Or see this region's nucleotide sequence