Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1615 and Shew_1616 overlap by 4 nucleotides Shew_1616 and Shew_1617 are separated by 56 nucleotides Shew_1617 and Shew_1618 are separated by 99 nucleotides
Shew_1615: Shew_1615 - ABC transporter-related protein (RefSeq), at 1,846,913 to 1,847,818
_1615
Shew_1616: Shew_1616 - hypothetical protein (RefSeq), at 1,847,815 to 1,848,669
_1616
Shew_1617: Shew_1617 - hypothetical protein (RefSeq), at 1,848,726 to 1,849,373
_1617
Shew_1618: Shew_1618 - hypothetical protein (RefSeq), at 1,849,473 to 1,850,306
_1618
Position (kb)
1848
1849
1850 Strain fitness (log2 ratio)
-1
0
1 at 1847.892 kb on + strand at 1848.020 kb on + strand, within Shew_1616 at 1848.035 kb on + strand, within Shew_1616 at 1848.615 kb on - strand at 1848.644 kb on - strand at 1848.954 kb on - strand, within Shew_1617 at 1849.277 kb on - strand, within Shew_1617 at 1850.024 kb on - strand, within Shew_1618 at 1850.074 kb on + strand, within Shew_1618 at 1850.074 kb on + strand, within Shew_1618 at 1850.082 kb on - strand, within Shew_1618 at 1850.273 kb on + strand at 1850.273 kb on + strand at 1850.281 kb on - strand at 1850.281 kb on - strand at 1850.320 kb on + strand at 1850.373 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 1,847,892 + -0.1 1,848,020 + Shew_1616 0.24 +0.3 1,848,035 + Shew_1616 0.26 +0.4 1,848,615 - -0.0 1,848,644 - -0.2 1,848,954 - Shew_1617 0.35 -0.1 1,849,277 - Shew_1617 0.85 -0.7 1,850,024 - Shew_1618 0.66 +1.0 1,850,074 + Shew_1618 0.72 +0.2 1,850,074 + Shew_1618 0.72 -0.1 1,850,082 - Shew_1618 0.73 -0.3 1,850,273 + -0.2 1,850,273 + +0.3 1,850,281 - +0.1 1,850,281 - -0.8 1,850,320 + -0.8 1,850,373 + +0.2
Or see this region's nucleotide sequence