Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1364 and Shew_1365 are separated by 196 nucleotides Shew_1365 and fliF are separated by 58 nucleotides fliF and fliG overlap by 8 nucleotides
Shew_1364: Shew_1364 - two component, sigma54 specific, Fis family transcriptional regulator (RefSeq), at 1,573,108 to 1,574,451
_1364
Shew_1365: Shew_1365 - flagellar hook-basal body complex subunit FliE (RefSeq), at 1,574,648 to 1,574,980
_1365
Shew_1366: fliF - flagellar MS-ring protein (RefSeq), at 1,575,039 to 1,576,736
fliF
Shew_1367: fliG - flagellar motor switch protein G (RefSeq), at 1,576,729 to 1,577,772
fliG
Position (kb)
1575
1576
1577 Strain fitness (log2 ratio)
-1
0
1 at 1574.174 kb on + strand, within Shew_1364 at 1574.298 kb on + strand, within Shew_1364 at 1574.306 kb on - strand, within Shew_1364 at 1574.343 kb on + strand at 1574.837 kb on + strand, within Shew_1365 at 1574.919 kb on + strand, within Shew_1365 at 1575.120 kb on + strand at 1575.604 kb on - strand, within fliF at 1575.616 kb on + strand, within fliF at 1575.668 kb on + strand, within fliF at 1575.673 kb on + strand, within fliF at 1576.390 kb on - strand, within fliF at 1576.456 kb on + strand, within fliF at 1577.172 kb on + strand, within fliG at 1577.180 kb on - strand, within fliG
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 1,574,174 + Shew_1364 0.79 +0.0 1,574,298 + Shew_1364 0.89 -0.7 1,574,306 - Shew_1364 0.89 -0.0 1,574,343 + -0.4 1,574,837 + Shew_1365 0.57 -0.3 1,574,919 + Shew_1365 0.81 +0.3 1,575,120 + -0.2 1,575,604 - fliF Shew_1366 0.33 +0.2 1,575,616 + fliF Shew_1366 0.34 -0.4 1,575,668 + fliF Shew_1366 0.37 -0.8 1,575,673 + fliF Shew_1366 0.37 -0.5 1,576,390 - fliF Shew_1366 0.80 +0.1 1,576,456 + fliF Shew_1366 0.83 -1.0 1,577,172 + fliG Shew_1367 0.42 -0.6 1,577,180 - fliG Shew_1367 0.43 +0.2
Or see this region's nucleotide sequence