Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rho and Shew_0338 are separated by 230 nucleotides Shew_0338 and Shew_0339 are separated by 171 nucleotides Shew_0339 and Shew_0340 overlap by 1 nucleotides
Shew_0337: rho - transcription termination factor Rho (RefSeq), at 382,589 to 383,854
rho
Shew_0338: Shew_0338 - thioredoxin (RefSeq), at 384,085 to 384,411
_0338
Shew_0339: Shew_0339 - ATP-dependent RNA helicase RhlB (RefSeq), at 384,583 to 385,890
_0339
Shew_0340: Shew_0340 - Ppx/GppA phosphatase (RefSeq), at 385,890 to 386,822
_0340
Position (kb) 384
385
386 Strain fitness (log2 ratio) -1
0
1 at 383.871 kb on - strand at 383.871 kb on - strand at 384.085 kb on + strand at 384.091 kb on - strand at 384.673 kb on - strand at 384.690 kb on + strand at 384.818 kb on + strand, within Shew_0339 at 384.847 kb on - strand, within Shew_0339 at 384.917 kb on + strand, within Shew_0339 at 384.934 kb on + strand, within Shew_0339 at 384.948 kb on + strand, within Shew_0339 at 385.403 kb on - strand, within Shew_0339 at 385.405 kb on + strand, within Shew_0339 at 385.453 kb on - strand, within Shew_0339 at 385.555 kb on - strand, within Shew_0339 at 385.739 kb on + strand, within Shew_0339 at 386.132 kb on + strand, within Shew_0340 at 386.207 kb on - strand, within Shew_0340 at 386.559 kb on + strand, within Shew_0340
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 383,871 - +0.8 383,871 - +0.4 384,085 + -0.0 384,091 - -0.4 384,673 - +0.4 384,690 + +0.2 384,818 + Shew_0339 0.18 -0.1 384,847 - Shew_0339 0.20 -0.5 384,917 + Shew_0339 0.26 -0.1 384,934 + Shew_0339 0.27 -0.3 384,948 + Shew_0339 0.28 -0.4 385,403 - Shew_0339 0.63 -0.5 385,405 + Shew_0339 0.63 -0.6 385,453 - Shew_0339 0.67 -0.2 385,555 - Shew_0339 0.74 -0.4 385,739 + Shew_0339 0.88 -0.3 386,132 + Shew_0340 0.26 +0.1 386,207 - Shew_0340 0.34 -0.1 386,559 + Shew_0340 0.72 +0.2
Or see this region's nucleotide sequence