Experiment: m.b. Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2087 and Dshi_2088 are separated by 373 nucleotides Dshi_2088 and Dshi_2089 are separated by 87 nucleotides Dshi_2089 and Dshi_2090 overlap by 4 nucleotides
Dshi_2087: Dshi_2087 - valyl-tRNA synthetase (RefSeq), at 2,209,300 to 2,212,413
_2087
Dshi_2088: Dshi_2088 - aminotransferase class I and II (RefSeq), at 2,212,787 to 2,213,905
_2088
Dshi_2089: Dshi_2089 - hypothetical protein (RefSeq), at 2,213,993 to 2,214,208
_2089
Dshi_2090: Dshi_2090 - Serine--glyoxylate transaminase (RefSeq), at 2,214,205 to 2,215,461
_2090
Position (kb)
2212
2213
2214 Strain fitness (log2 ratio)
-1
0
1 at 2212.563 kb on + strand at 2212.571 kb on - strand at 2212.617 kb on - strand at 2212.965 kb on - strand, within Dshi_2088 at 2213.134 kb on - strand, within Dshi_2088 at 2213.240 kb on + strand, within Dshi_2088 at 2213.293 kb on + strand, within Dshi_2088 at 2213.295 kb on - strand, within Dshi_2088 at 2213.386 kb on - strand, within Dshi_2088 at 2213.476 kb on + strand, within Dshi_2088 at 2213.669 kb on + strand, within Dshi_2088 at 2213.723 kb on + strand, within Dshi_2088 at 2213.877 kb on - strand at 2214.050 kb on - strand, within Dshi_2089 at 2214.271 kb on + strand at 2214.325 kb on - strand at 2214.411 kb on - strand, within Dshi_2090 at 2214.536 kb on - strand, within Dshi_2090 at 2214.650 kb on + strand, within Dshi_2090 at 2214.704 kb on + strand, within Dshi_2090 at 2214.708 kb on + strand, within Dshi_2090 at 2214.742 kb on - strand, within Dshi_2090
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cisplatin 0.05 mg/ml remove 2,212,563 + +0.4 2,212,571 - -0.5 2,212,617 - -0.3 2,212,965 - Dshi_2088 0.16 -0.4 2,213,134 - Dshi_2088 0.31 -0.1 2,213,240 + Dshi_2088 0.40 -0.1 2,213,293 + Dshi_2088 0.45 -0.7 2,213,295 - Dshi_2088 0.45 +0.4 2,213,386 - Dshi_2088 0.54 +0.4 2,213,476 + Dshi_2088 0.62 -1.1 2,213,669 + Dshi_2088 0.79 -0.5 2,213,723 + Dshi_2088 0.84 -0.2 2,213,877 - -1.1 2,214,050 - Dshi_2089 0.26 -0.5 2,214,271 + -0.8 2,214,325 - +0.2 2,214,411 - Dshi_2090 0.16 +1.5 2,214,536 - Dshi_2090 0.26 -1.7 2,214,650 + Dshi_2090 0.35 -0.1 2,214,704 + Dshi_2090 0.40 -0.3 2,214,708 + Dshi_2090 0.40 -0.1 2,214,742 - Dshi_2090 0.43 +0.3
Or see this region's nucleotide sequence