Experiment: m.b. Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1198 and Dshi_1199 are separated by 152 nucleotides Dshi_1199 and Dshi_1200 are separated by 2 nucleotides Dshi_1200 and Dshi_1201 are separated by 88 nucleotides Dshi_1201 and Dshi_R0025 are separated by 51 nucleotides Dshi_R0025 and Dshi_1202 are separated by 18 nucleotides
Dshi_1198: Dshi_1198 - beta-lactamase (RefSeq), at 1,238,964 to 1,239,965
_1198
Dshi_1199: Dshi_1199 - Inositol-phosphate phosphatase (RefSeq), at 1,240,118 to 1,240,912
_1199
Dshi_1200: Dshi_1200 - Rhomboid family protein (RefSeq), at 1,240,915 to 1,241,691
_1200
Dshi_1201: Dshi_1201 - Tetratricopeptide TPR_2 repeat protein (RefSeq), at 1,241,780 to 1,242,331
_1201
Dshi_R0025: Dshi_R0025 - tRNA-Leu (RefSeq), at 1,242,383 to 1,242,468
_R0025
Dshi_1202: Dshi_1202 - heat shock protein DnaJ domain protein (RefSeq), at 1,242,487 to 1,243,116
_1202
Position (kb)
1240
1241
1242 Strain fitness (log2 ratio)
-1
0
1 at 1240.014 kb on - strand at 1240.025 kb on - strand at 1240.956 kb on + strand at 1241.158 kb on + strand, within Dshi_1200 at 1241.680 kb on + strand at 1241.691 kb on - strand at 1241.700 kb on + strand at 1241.705 kb on + strand at 1242.039 kb on + strand, within Dshi_1201 at 1242.098 kb on - strand, within Dshi_1201 at 1242.214 kb on - strand, within Dshi_1201 at 1242.481 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cisplatin 0.05 mg/ml remove 1,240,014 - -0.1 1,240,025 - +0.3 1,240,956 + -0.4 1,241,158 + Dshi_1200 0.31 -0.6 1,241,680 + +0.1 1,241,691 - +1.3 1,241,700 + -0.4 1,241,705 + -1.3 1,242,039 + Dshi_1201 0.47 -0.2 1,242,098 - Dshi_1201 0.58 -0.7 1,242,214 - Dshi_1201 0.79 -0.7 1,242,481 - -0.0
Or see this region's nucleotide sequence