Experiment: m.b. Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0183 and Dshi_0184 overlap by 4 nucleotides Dshi_0184 and Dshi_0185 are separated by 39 nucleotides Dshi_0185 and Dshi_0186 overlap by 4 nucleotides Dshi_0186 and Dshi_0187 are separated by 22 nucleotides
Dshi_0183: Dshi_0183 - Methyltransferase type 11 (RefSeq), at 185,656 to 186,255
_0183
Dshi_0184: Dshi_0184 - hypothetical protein (RefSeq), at 186,252 to 186,704
_0184
Dshi_0185: Dshi_0185 - phosphatidate cytidylyltransferase (RefSeq), at 186,744 to 187,694
_0185
Dshi_0186: Dshi_0186 - phospholipid/glycerol acyltransferase (RefSeq), at 187,691 to 188,344
_0186
Dshi_0187: Dshi_0187 - Lysine exporter protein (LYSE/YGGA) (RefSeq), at 188,367 to 188,981
_0187
Position (kb)
186
187
188 Strain fitness (log2 ratio)
-2
-1
0
1 at 185.801 kb on + strand, within Dshi_0183 at 185.801 kb on + strand, within Dshi_0183 at 185.844 kb on + strand, within Dshi_0183 at 186.169 kb on - strand, within Dshi_0183 at 186.425 kb on + strand, within Dshi_0184 at 186.627 kb on - strand, within Dshi_0184 at 186.741 kb on + strand at 186.749 kb on - strand at 186.749 kb on - strand at 186.855 kb on + strand, within Dshi_0185 at 186.952 kb on + strand, within Dshi_0185 at 186.952 kb on + strand, within Dshi_0185 at 186.977 kb on + strand, within Dshi_0185 at 187.004 kb on - strand, within Dshi_0185 at 187.065 kb on - strand, within Dshi_0185 at 187.140 kb on - strand, within Dshi_0185 at 187.325 kb on - strand, within Dshi_0185 at 187.330 kb on - strand, within Dshi_0185 at 187.335 kb on - strand, within Dshi_0185 at 187.352 kb on - strand, within Dshi_0185 at 187.543 kb on + strand, within Dshi_0185 at 187.774 kb on + strand at 187.806 kb on + strand, within Dshi_0186 at 187.814 kb on - strand, within Dshi_0186 at 188.180 kb on + strand, within Dshi_0186 at 188.411 kb on - strand at 188.618 kb on + strand, within Dshi_0187
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cisplatin 0.05 mg/ml remove 185,801 + Dshi_0183 0.24 -2.1 185,801 + Dshi_0183 0.24 -0.1 185,844 + Dshi_0183 0.31 -1.2 186,169 - Dshi_0183 0.85 -0.8 186,425 + Dshi_0184 0.38 -2.6 186,627 - Dshi_0184 0.83 -0.0 186,741 + -1.5 186,749 - +0.6 186,749 - -0.3 186,855 + Dshi_0185 0.12 -0.1 186,952 + Dshi_0185 0.22 +0.8 186,952 + Dshi_0185 0.22 -0.9 186,977 + Dshi_0185 0.25 -1.7 187,004 - Dshi_0185 0.27 +0.6 187,065 - Dshi_0185 0.34 +0.5 187,140 - Dshi_0185 0.42 +0.1 187,325 - Dshi_0185 0.61 -0.9 187,330 - Dshi_0185 0.62 -0.9 187,335 - Dshi_0185 0.62 +0.3 187,352 - Dshi_0185 0.64 -0.8 187,543 + Dshi_0185 0.84 -0.4 187,774 + +1.1 187,806 + Dshi_0186 0.18 -0.8 187,814 - Dshi_0186 0.19 -0.9 188,180 + Dshi_0186 0.75 +0.1 188,411 - -0.7 188,618 + Dshi_0187 0.41 -0.9
Or see this region's nucleotide sequence