Experiment: m.b. Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0085 and Dshi_0086 are separated by 311 nucleotides Dshi_0086 and Dshi_0087 are separated by 198 nucleotides Dshi_0087 and Dshi_0088 are separated by 18 nucleotides
Dshi_0085: Dshi_0085 - 3-isopropylmalate dehydratase, large subunit (RefSeq), at 83,697 to 85,100
_0085
Dshi_0086: Dshi_0086 - SOUL heme-binding protein (RefSeq), at 85,412 to 86,014
_0086
Dshi_0087: Dshi_0087 - iojap-like protein (RefSeq), at 86,213 to 86,545
_0087
Dshi_0088: Dshi_0088 - protein of unknown function DUF163 (RefSeq), at 86,564 to 87,034
_0088
Position (kb)
85
86
87 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 85.053 kb on + strand at 85.292 kb on - strand at 85.305 kb on - strand at 85.319 kb on - strand at 85.333 kb on - strand at 85.333 kb on - strand at 85.366 kb on + strand at 85.367 kb on + strand at 85.374 kb on - strand at 85.378 kb on + strand at 85.427 kb on + strand at 85.433 kb on + strand at 85.446 kb on + strand at 85.465 kb on + strand at 85.562 kb on + strand, within Dshi_0086 at 85.586 kb on + strand, within Dshi_0086 at 85.656 kb on + strand, within Dshi_0086 at 85.656 kb on + strand, within Dshi_0086 at 85.666 kb on + strand, within Dshi_0086 at 85.674 kb on - strand, within Dshi_0086 at 85.683 kb on + strand, within Dshi_0086 at 85.750 kb on + strand, within Dshi_0086 at 85.815 kb on - strand, within Dshi_0086 at 85.836 kb on - strand, within Dshi_0086 at 85.843 kb on + strand, within Dshi_0086 at 85.917 kb on + strand, within Dshi_0086 at 85.917 kb on + strand, within Dshi_0086 at 85.917 kb on + strand, within Dshi_0086 at 85.925 kb on - strand, within Dshi_0086 at 85.925 kb on - strand, within Dshi_0086 at 85.925 kb on - strand, within Dshi_0086 at 85.925 kb on - strand, within Dshi_0086 at 85.925 kb on - strand, within Dshi_0086 at 85.925 kb on - strand, within Dshi_0086 at 85.925 kb on - strand, within Dshi_0086 at 85.925 kb on - strand, within Dshi_0086 at 85.925 kb on - strand, within Dshi_0086 at 85.925 kb on - strand, within Dshi_0086 at 85.971 kb on - strand at 85.972 kb on + strand at 85.980 kb on - strand at 85.980 kb on - strand at 85.994 kb on + strand at 86.033 kb on - strand at 86.052 kb on + strand at 86.055 kb on - strand at 86.081 kb on - strand at 86.205 kb on - strand at 86.244 kb on - strand at 86.246 kb on - strand at 86.247 kb on + strand, within Dshi_0087 at 86.247 kb on + strand, within Dshi_0087 at 86.255 kb on - strand, within Dshi_0087 at 86.257 kb on + strand, within Dshi_0087 at 86.257 kb on + strand, within Dshi_0087 at 86.257 kb on + strand, within Dshi_0087 at 86.257 kb on + strand, within Dshi_0087 at 86.257 kb on + strand, within Dshi_0087 at 86.265 kb on - strand, within Dshi_0087 at 86.265 kb on - strand, within Dshi_0087 at 86.265 kb on - strand, within Dshi_0087 at 86.288 kb on - strand, within Dshi_0087 at 86.314 kb on + strand, within Dshi_0087 at 86.322 kb on - strand, within Dshi_0087 at 86.383 kb on + strand, within Dshi_0087 at 86.383 kb on + strand, within Dshi_0087 at 86.391 kb on - strand, within Dshi_0087 at 86.391 kb on - strand, within Dshi_0087 at 86.402 kb on - strand, within Dshi_0087 at 86.414 kb on + strand, within Dshi_0087 at 86.419 kb on + strand, within Dshi_0087 at 86.420 kb on + strand, within Dshi_0087 at 86.426 kb on + strand, within Dshi_0087 at 86.439 kb on - strand, within Dshi_0087 at 86.496 kb on + strand, within Dshi_0087 at 86.542 kb on - strand at 86.611 kb on - strand, within Dshi_0088 at 86.709 kb on - strand, within Dshi_0088 at 86.745 kb on + strand, within Dshi_0088 at 86.753 kb on - strand, within Dshi_0088 at 86.777 kb on + strand, within Dshi_0088 at 86.823 kb on + strand, within Dshi_0088 at 86.863 kb on - strand, within Dshi_0088 at 86.877 kb on + strand, within Dshi_0088 at 86.991 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cisplatin 0.05 mg/ml remove 85,053 + +0.7 85,292 - +0.9 85,305 - -1.0 85,319 - +0.1 85,333 - -2.9 85,333 - +1.4 85,366 + +0.6 85,367 + -0.1 85,374 - -0.3 85,378 + +0.9 85,427 + -0.5 85,433 + +0.4 85,446 + +1.0 85,465 + -0.1 85,562 + Dshi_0086 0.25 -0.3 85,586 + Dshi_0086 0.29 -2.2 85,656 + Dshi_0086 0.40 +0.7 85,656 + Dshi_0086 0.40 -0.6 85,666 + Dshi_0086 0.42 -0.7 85,674 - Dshi_0086 0.43 -1.2 85,683 + Dshi_0086 0.45 -1.0 85,750 + Dshi_0086 0.56 -0.7 85,815 - Dshi_0086 0.67 -0.2 85,836 - Dshi_0086 0.70 -0.5 85,843 + Dshi_0086 0.71 +2.7 85,917 + Dshi_0086 0.84 -2.5 85,917 + Dshi_0086 0.84 -0.2 85,917 + Dshi_0086 0.84 -0.2 85,925 - Dshi_0086 0.85 +0.6 85,925 - Dshi_0086 0.85 +0.1 85,925 - Dshi_0086 0.85 -1.5 85,925 - Dshi_0086 0.85 -1.5 85,925 - Dshi_0086 0.85 +0.2 85,925 - Dshi_0086 0.85 +1.1 85,925 - Dshi_0086 0.85 -0.6 85,925 - Dshi_0086 0.85 +0.4 85,925 - Dshi_0086 0.85 +0.1 85,925 - Dshi_0086 0.85 +0.4 85,971 - +0.4 85,972 + -1.1 85,980 - -0.2 85,980 - -1.1 85,994 + -0.6 86,033 - -1.5 86,052 + -0.5 86,055 - -0.2 86,081 - +0.0 86,205 - -0.1 86,244 - +0.8 86,246 - -1.2 86,247 + Dshi_0087 0.10 -0.9 86,247 + Dshi_0087 0.10 -0.9 86,255 - Dshi_0087 0.13 +0.8 86,257 + Dshi_0087 0.13 -2.9 86,257 + Dshi_0087 0.13 +0.3 86,257 + Dshi_0087 0.13 -0.3 86,257 + Dshi_0087 0.13 +0.1 86,257 + Dshi_0087 0.13 -1.9 86,265 - Dshi_0087 0.16 +0.1 86,265 - Dshi_0087 0.16 -0.2 86,265 - Dshi_0087 0.16 -1.0 86,288 - Dshi_0087 0.23 -0.8 86,314 + Dshi_0087 0.30 -0.2 86,322 - Dshi_0087 0.33 -2.5 86,383 + Dshi_0087 0.51 +0.3 86,383 + Dshi_0087 0.51 +0.4 86,391 - Dshi_0087 0.53 +0.6 86,391 - Dshi_0087 0.53 +0.8 86,402 - Dshi_0087 0.57 +0.1 86,414 + Dshi_0087 0.60 +0.8 86,419 + Dshi_0087 0.62 -0.2 86,420 + Dshi_0087 0.62 -0.8 86,426 + Dshi_0087 0.64 +0.2 86,439 - Dshi_0087 0.68 +0.1 86,496 + Dshi_0087 0.85 -1.2 86,542 - -0.9 86,611 - Dshi_0088 0.10 +0.3 86,709 - Dshi_0088 0.31 +0.2 86,745 + Dshi_0088 0.38 +0.1 86,753 - Dshi_0088 0.40 -0.5 86,777 + Dshi_0088 0.45 +0.1 86,823 + Dshi_0088 0.55 -2.5 86,863 - Dshi_0088 0.63 -0.5 86,877 + Dshi_0088 0.66 -2.6 86,991 - +1.1
Or see this region's nucleotide sequence