Experiment: BHIS with Ceftazidime 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4023 and BT4024 overlap by 4 nucleotides BT4024 and BT4025 are separated by 21 nucleotides BT4025 and BT4026 overlap by 4 nucleotides BT4026 and BT4027 are separated by 26 nucleotides BT4027 and BT4028 overlap by 4 nucleotides BT4028 and BT4029 are separated by 143 nucleotides
BT4023: BT4023 - transposase (NCBI ptt file), at 5,240,134 to 5,240,490
BT4023
BT4024: BT4024 - hypothetical protein (NCBI ptt file), at 5,240,487 to 5,240,747
BT4024
BT4025: BT4025 - hypothetical protein (NCBI ptt file), at 5,240,769 to 5,241,065
BT4025
BT4026: BT4026 - conserved hypothetical protein (NCBI ptt file), at 5,241,062 to 5,242,060
BT4026
BT4027: BT4027 - hypothetical protein (NCBI ptt file), at 5,242,087 to 5,242,314
BT4027
BT4028: BT4028 - hypothetical protein (NCBI ptt file), at 5,242,311 to 5,242,583
BT4028
BT4029: BT4029 - hypothetical protein (NCBI ptt file), at 5,242,727 to 5,243,467
BT4029
Position (kb) 5241
5242
5243 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5240.194 kb on - strand, within BT4023 at 5240.202 kb on - strand, within BT4023 at 5240.223 kb on - strand, within BT4023 at 5240.236 kb on - strand, within BT4023 at 5240.266 kb on - strand, within BT4023 at 5240.266 kb on - strand, within BT4023 at 5240.268 kb on - strand, within BT4023 at 5240.356 kb on - strand, within BT4023 at 5240.554 kb on - strand, within BT4024 at 5240.619 kb on - strand, within BT4024 at 5240.666 kb on - strand, within BT4024 at 5240.666 kb on - strand, within BT4024 at 5240.717 kb on - strand, within BT4024 at 5240.728 kb on - strand at 5240.743 kb on - strand at 5240.753 kb on - strand at 5240.763 kb on - strand at 5240.922 kb on - strand, within BT4025 at 5240.986 kb on - strand, within BT4025 at 5241.034 kb on - strand, within BT4025 at 5241.067 kb on + strand at 5241.068 kb on - strand at 5241.086 kb on + strand at 5241.123 kb on + strand at 5241.124 kb on - strand at 5241.158 kb on + strand at 5241.167 kb on + strand, within BT4026 at 5241.209 kb on + strand, within BT4026 at 5241.210 kb on - strand, within BT4026 at 5241.345 kb on + strand, within BT4026 at 5241.345 kb on + strand, within BT4026 at 5241.415 kb on - strand, within BT4026 at 5241.436 kb on - strand, within BT4026 at 5241.512 kb on - strand, within BT4026 at 5241.655 kb on + strand, within BT4026 at 5241.715 kb on + strand, within BT4026 at 5241.716 kb on - strand, within BT4026 at 5241.721 kb on - strand, within BT4026 at 5241.737 kb on - strand, within BT4026 at 5241.886 kb on - strand, within BT4026 at 5241.931 kb on + strand, within BT4026 at 5241.931 kb on + strand, within BT4026 at 5241.978 kb on + strand at 5242.098 kb on + strand at 5242.117 kb on + strand, within BT4027 at 5242.118 kb on - strand, within BT4027 at 5242.138 kb on - strand, within BT4027 at 5242.139 kb on + strand, within BT4027 at 5242.140 kb on - strand, within BT4027 at 5242.423 kb on - strand, within BT4028 at 5242.582 kb on - strand at 5242.615 kb on + strand at 5242.739 kb on - strand at 5242.760 kb on - strand at 5242.821 kb on - strand, within BT4029
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Ceftazidime 0.5 mg/ml remove 5,240,194 - BT4023 0.17 +1.6 5,240,202 - BT4023 0.19 -2.1 5,240,223 - BT4023 0.25 +1.0 5,240,236 - BT4023 0.29 +0.6 5,240,266 - BT4023 0.37 -0.2 5,240,266 - BT4023 0.37 -1.2 5,240,268 - BT4023 0.38 -1.0 5,240,356 - BT4023 0.62 +0.6 5,240,554 - BT4024 0.26 +0.0 5,240,619 - BT4024 0.51 -2.0 5,240,666 - BT4024 0.69 +2.2 5,240,666 - BT4024 0.69 -1.3 5,240,717 - BT4024 0.88 -1.4 5,240,728 - -1.6 5,240,743 - -1.5 5,240,753 - +1.0 5,240,763 - -1.0 5,240,922 - BT4025 0.52 -1.6 5,240,986 - BT4025 0.73 +1.4 5,241,034 - BT4025 0.89 -1.6 5,241,067 + +1.6 5,241,068 - +0.0 5,241,086 + -2.7 5,241,123 + -0.6 5,241,124 - -1.8 5,241,158 + -1.2 5,241,167 + BT4026 0.11 -1.0 5,241,209 + BT4026 0.15 -1.4 5,241,210 - BT4026 0.15 -0.5 5,241,345 + BT4026 0.28 -0.5 5,241,345 + BT4026 0.28 -2.6 5,241,415 - BT4026 0.35 -0.5 5,241,436 - BT4026 0.37 +1.6 5,241,512 - BT4026 0.45 -2.3 5,241,655 + BT4026 0.59 -0.4 5,241,715 + BT4026 0.65 +1.6 5,241,716 - BT4026 0.65 -2.6 5,241,721 - BT4026 0.66 -1.3 5,241,737 - BT4026 0.68 +1.1 5,241,886 - BT4026 0.82 -1.7 5,241,931 + BT4026 0.87 +0.0 5,241,931 + BT4026 0.87 -1.9 5,241,978 + -0.3 5,242,098 + +1.4 5,242,117 + BT4027 0.13 -0.7 5,242,118 - BT4027 0.14 -1.9 5,242,138 - BT4027 0.22 -1.5 5,242,139 + BT4027 0.23 -0.9 5,242,140 - BT4027 0.23 -1.4 5,242,423 - BT4028 0.41 -3.3 5,242,582 - -0.1 5,242,615 + -1.0 5,242,739 - -1.6 5,242,760 - -1.1 5,242,821 - BT4029 0.13 +0.6
Or see this region's nucleotide sequence