Experiment: BHIS with Ceftazidime 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1737 and BT1738 are separated by 64 nucleotides BT1738 and BT1739 are separated by 197 nucleotides
BT1737: BT1737 - conserved hypothetical protein, putative secreted protein (NCBI ptt file), at 2,140,641 to 2,142,149
BT1737
BT1738: BT1738 - two-component system sensor histidine kinase (NCBI ptt file), at 2,142,214 to 2,144,133
BT1738
BT1739: BT1739 - excinuclease ABC subunit A (NCBI ptt file), at 2,144,331 to 2,147,102
BT1739
Position (kb)
2142
2143
2144
2145 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2141.242 kb on - strand, within BT1737 at 2141.242 kb on - strand, within BT1737 at 2141.323 kb on + strand, within BT1737 at 2141.323 kb on + strand, within BT1737 at 2141.460 kb on + strand, within BT1737 at 2141.460 kb on + strand, within BT1737 at 2141.461 kb on - strand, within BT1737 at 2141.561 kb on - strand, within BT1737 at 2141.567 kb on - strand at 2141.589 kb on + strand, within BT1737 at 2141.590 kb on - strand, within BT1737 at 2141.590 kb on - strand, within BT1737 at 2141.630 kb on + strand, within BT1737 at 2141.680 kb on + strand, within BT1737 at 2141.711 kb on - strand, within BT1737 at 2141.711 kb on - strand, within BT1737 at 2141.899 kb on + strand, within BT1737 at 2142.063 kb on - strand at 2142.120 kb on + strand at 2142.123 kb on + strand at 2142.186 kb on + strand at 2142.202 kb on + strand at 2142.203 kb on - strand at 2142.465 kb on + strand, within BT1738 at 2142.486 kb on + strand, within BT1738 at 2142.571 kb on - strand, within BT1738 at 2142.616 kb on + strand, within BT1738 at 2142.641 kb on - strand, within BT1738 at 2142.664 kb on - strand, within BT1738 at 2142.692 kb on - strand, within BT1738 at 2142.693 kb on + strand, within BT1738 at 2142.725 kb on + strand, within BT1738 at 2142.756 kb on - strand, within BT1738 at 2142.849 kb on + strand, within BT1738 at 2142.940 kb on - strand, within BT1738 at 2142.940 kb on - strand, within BT1738 at 2142.942 kb on - strand, within BT1738 at 2142.968 kb on - strand, within BT1738 at 2143.000 kb on + strand, within BT1738 at 2143.003 kb on - strand, within BT1738 at 2143.078 kb on + strand, within BT1738 at 2143.128 kb on + strand, within BT1738 at 2143.262 kb on + strand, within BT1738 at 2143.420 kb on - strand, within BT1738 at 2143.420 kb on - strand, within BT1738 at 2143.442 kb on + strand, within BT1738 at 2143.453 kb on - strand, within BT1738 at 2143.453 kb on - strand, within BT1738 at 2143.480 kb on - strand, within BT1738 at 2143.698 kb on - strand, within BT1738 at 2143.809 kb on + strand, within BT1738 at 2143.829 kb on + strand, within BT1738 at 2143.871 kb on + strand, within BT1738 at 2143.871 kb on + strand, within BT1738 at 2143.872 kb on - strand, within BT1738 at 2143.872 kb on - strand, within BT1738 at 2143.877 kb on + strand, within BT1738 at 2143.877 kb on + strand, within BT1738 at 2143.878 kb on - strand, within BT1738 at 2144.001 kb on - strand at 2144.010 kb on + strand at 2144.082 kb on + strand at 2144.085 kb on + strand at 2144.085 kb on + strand at 2144.086 kb on - strand at 2144.120 kb on - strand at 2144.124 kb on + strand at 2144.125 kb on - strand at 2144.125 kb on - strand at 2144.125 kb on - strand at 2144.125 kb on - strand at 2144.206 kb on - strand at 2144.215 kb on - strand at 2144.331 kb on - strand at 2144.475 kb on + strand at 2144.496 kb on + strand at 2144.511 kb on - strand at 2144.653 kb on - strand, within BT1739 at 2144.694 kb on + strand, within BT1739 at 2144.694 kb on + strand, within BT1739 at 2144.694 kb on + strand, within BT1739 at 2144.694 kb on + strand, within BT1739 at 2144.695 kb on - strand, within BT1739 at 2144.782 kb on - strand, within BT1739 at 2144.783 kb on + strand, within BT1739 at 2144.843 kb on - strand, within BT1739 at 2144.895 kb on - strand, within BT1739 at 2144.904 kb on + strand, within BT1739 at 2144.931 kb on + strand, within BT1739 at 2145.032 kb on + strand, within BT1739 at 2145.033 kb on - strand, within BT1739 at 2145.033 kb on - strand, within BT1739 at 2145.033 kb on - strand, within BT1739
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Ceftazidime 0.5 mg/ml remove 2,141,242 - BT1737 0.40 +1.1 2,141,242 - BT1737 0.40 -2.3 2,141,323 + BT1737 0.45 -2.1 2,141,323 + BT1737 0.45 +0.2 2,141,460 + BT1737 0.54 +2.0 2,141,460 + BT1737 0.54 +0.3 2,141,461 - BT1737 0.54 -2.2 2,141,561 - BT1737 0.61 -1.9 2,141,567 - +0.5 2,141,589 + BT1737 0.63 -1.3 2,141,590 - BT1737 0.63 +0.9 2,141,590 - BT1737 0.63 +0.4 2,141,630 + BT1737 0.66 +0.9 2,141,680 + BT1737 0.69 -0.1 2,141,711 - BT1737 0.71 -0.1 2,141,711 - BT1737 0.71 +0.5 2,141,899 + BT1737 0.83 +1.2 2,142,063 - -1.1 2,142,120 + +1.5 2,142,123 + -1.8 2,142,186 + -3.0 2,142,202 + -0.2 2,142,203 - -1.5 2,142,465 + BT1738 0.13 -0.7 2,142,486 + BT1738 0.14 -1.1 2,142,571 - BT1738 0.19 -1.0 2,142,616 + BT1738 0.21 +0.5 2,142,641 - BT1738 0.22 +1.5 2,142,664 - BT1738 0.23 -2.2 2,142,692 - BT1738 0.25 +1.5 2,142,693 + BT1738 0.25 -2.3 2,142,725 + BT1738 0.27 -0.5 2,142,756 - BT1738 0.28 -0.5 2,142,849 + BT1738 0.33 -3.0 2,142,940 - BT1738 0.38 -0.2 2,142,940 - BT1738 0.38 -1.5 2,142,942 - BT1738 0.38 +0.2 2,142,968 - BT1738 0.39 -0.1 2,143,000 + BT1738 0.41 +0.9 2,143,003 - BT1738 0.41 -0.8 2,143,078 + BT1738 0.45 -0.8 2,143,128 + BT1738 0.48 +1.5 2,143,262 + BT1738 0.55 -1.2 2,143,420 - BT1738 0.63 -0.9 2,143,420 - BT1738 0.63 +0.5 2,143,442 + BT1738 0.64 +0.1 2,143,453 - BT1738 0.65 -0.8 2,143,453 - BT1738 0.65 -2.0 2,143,480 - BT1738 0.66 -2.4 2,143,698 - BT1738 0.77 +0.1 2,143,809 + BT1738 0.83 -0.5 2,143,829 + BT1738 0.84 -2.4 2,143,871 + BT1738 0.86 +0.4 2,143,871 + BT1738 0.86 -0.5 2,143,872 - BT1738 0.86 -1.5 2,143,872 - BT1738 0.86 -0.1 2,143,877 + BT1738 0.87 -1.1 2,143,877 + BT1738 0.87 -0.6 2,143,878 - BT1738 0.87 -2.4 2,144,001 - +0.6 2,144,010 + +0.5 2,144,082 + +0.2 2,144,085 + -1.1 2,144,085 + +1.1 2,144,086 - -1.3 2,144,120 - +0.2 2,144,124 + -1.3 2,144,125 - +0.9 2,144,125 - -0.7 2,144,125 - -0.1 2,144,125 - +0.5 2,144,206 - -1.1 2,144,215 - +0.5 2,144,331 - -1.3 2,144,475 + +0.6 2,144,496 + -1.3 2,144,511 - -0.5 2,144,653 - BT1739 0.12 +0.7 2,144,694 + BT1739 0.13 -0.7 2,144,694 + BT1739 0.13 -0.8 2,144,694 + BT1739 0.13 -1.3 2,144,694 + BT1739 0.13 -3.0 2,144,695 - BT1739 0.13 -0.5 2,144,782 - BT1739 0.16 -0.1 2,144,783 + BT1739 0.16 -0.1 2,144,843 - BT1739 0.18 -1.0 2,144,895 - BT1739 0.20 +1.5 2,144,904 + BT1739 0.21 +0.2 2,144,931 + BT1739 0.22 -1.8 2,145,032 + BT1739 0.25 +0.5 2,145,033 - BT1739 0.25 -0.1 2,145,033 - BT1739 0.25 +0.9 2,145,033 - BT1739 0.25 -1.1
Or see this region's nucleotide sequence