Experiment: BHIS with Ceftazidime 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0064 and BT0065 are separated by 69 nucleotides BT0065 and BT0066 are separated by 114 nucleotides
BT0064: BT0064 - hypothetical protein (NCBI ptt file), at 59,377 to 61,551
BT0064
BT0065: BT0065 - alpha-galactosidase precursor (NCBI ptt file), at 61,621 to 64,158
BT0065
BT0066: BT0066 - major outer membrane protein OmpA (NCBI ptt file), at 64,273 to 65,475
BT0066
Position (kb) 61
62
63
64
65 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 60.646 kb on - strand, within BT0064 at 60.648 kb on + strand, within BT0064 at 60.780 kb on - strand, within BT0064 at 60.784 kb on - strand, within BT0064 at 60.972 kb on - strand, within BT0064 at 60.982 kb on - strand, within BT0064 at 60.982 kb on - strand, within BT0064 at 60.982 kb on - strand, within BT0064 at 60.988 kb on - strand, within BT0064 at 61.016 kb on - strand, within BT0064 at 61.048 kb on - strand, within BT0064 at 61.048 kb on - strand, within BT0064 at 61.095 kb on + strand, within BT0064 at 61.096 kb on - strand, within BT0064 at 61.300 kb on + strand, within BT0064 at 61.300 kb on + strand, within BT0064 at 61.303 kb on + strand, within BT0064 at 61.306 kb on + strand, within BT0064 at 61.332 kb on + strand, within BT0064 at 61.333 kb on - strand, within BT0064 at 61.606 kb on + strand at 61.606 kb on + strand at 61.631 kb on + strand at 61.853 kb on - strand at 61.926 kb on + strand, within BT0065 at 61.954 kb on + strand, within BT0065 at 61.961 kb on - strand, within BT0065 at 61.964 kb on - strand, within BT0065 at 62.461 kb on - strand, within BT0065 at 62.783 kb on - strand, within BT0065 at 62.812 kb on - strand, within BT0065 at 62.817 kb on + strand, within BT0065 at 62.908 kb on - strand, within BT0065 at 62.908 kb on - strand, within BT0065 at 62.999 kb on + strand, within BT0065 at 63.121 kb on + strand, within BT0065 at 63.143 kb on - strand, within BT0065 at 63.143 kb on - strand, within BT0065 at 63.257 kb on - strand, within BT0065 at 63.257 kb on - strand, within BT0065 at 63.259 kb on + strand, within BT0065 at 63.260 kb on - strand, within BT0065 at 63.410 kb on - strand, within BT0065 at 63.485 kb on - strand, within BT0065 at 63.772 kb on - strand, within BT0065 at 63.856 kb on - strand, within BT0065 at 63.923 kb on - strand at 63.982 kb on - strand at 63.995 kb on - strand at 64.032 kb on - strand at 64.059 kb on + strand at 64.241 kb on + strand at 64.364 kb on - strand at 64.368 kb on + strand at 64.378 kb on + strand at 64.378 kb on + strand at 64.379 kb on - strand at 64.444 kb on + strand, within BT0066 at 64.445 kb on - strand, within BT0066 at 64.553 kb on - strand, within BT0066 at 64.736 kb on - strand, within BT0066 at 64.977 kb on + strand, within BT0066 at 64.977 kb on + strand, within BT0066 at 64.978 kb on - strand, within BT0066 at 65.023 kb on + strand, within BT0066
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Ceftazidime 0.5 mg/ml remove 60,646 - BT0064 0.58 -1.1 60,648 + BT0064 0.58 -1.3 60,780 - BT0064 0.65 -0.2 60,784 - BT0064 0.65 +1.3 60,972 - BT0064 0.73 +0.3 60,982 - BT0064 0.74 +1.4 60,982 - BT0064 0.74 -1.2 60,982 - BT0064 0.74 -1.6 60,988 - BT0064 0.74 -0.8 61,016 - BT0064 0.75 +0.6 61,048 - BT0064 0.77 -0.1 61,048 - BT0064 0.77 -1.6 61,095 + BT0064 0.79 -0.7 61,096 - BT0064 0.79 -0.9 61,300 + BT0064 0.88 +2.4 61,300 + BT0064 0.88 -1.9 61,303 + BT0064 0.89 -0.5 61,306 + BT0064 0.89 +1.4 61,332 + BT0064 0.90 -1.2 61,333 - BT0064 0.90 -3.5 61,606 + -1.5 61,606 + +0.2 61,631 + +1.4 61,853 - +0.7 61,926 + BT0065 0.12 -0.6 61,954 + BT0065 0.13 -0.2 61,961 - BT0065 0.13 +0.0 61,964 - BT0065 0.14 -1.9 62,461 - BT0065 0.33 -1.9 62,783 - BT0065 0.46 -0.6 62,812 - BT0065 0.47 -2.0 62,817 + BT0065 0.47 -2.8 62,908 - BT0065 0.51 -1.6 62,908 - BT0065 0.51 -3.3 62,999 + BT0065 0.54 -4.2 63,121 + BT0065 0.59 -1.2 63,143 - BT0065 0.60 +1.0 63,143 - BT0065 0.60 -0.0 63,257 - BT0065 0.64 -1.6 63,257 - BT0065 0.64 +4.0 63,259 + BT0065 0.65 -2.9 63,260 - BT0065 0.65 +1.4 63,410 - BT0065 0.70 +1.4 63,485 - BT0065 0.73 -1.8 63,772 - BT0065 0.85 +0.6 63,856 - BT0065 0.88 -1.4 63,923 - +0.3 63,982 - -2.3 63,995 - -1.9 64,032 - -0.7 64,059 + -3.0 64,241 + -0.6 64,364 - +0.1 64,368 + +0.6 64,378 + -1.6 64,378 + -1.9 64,379 - -3.2 64,444 + BT0066 0.14 +0.4 64,445 - BT0066 0.14 +0.4 64,553 - BT0066 0.23 -0.6 64,736 - BT0066 0.38 -0.9 64,977 + BT0066 0.59 -3.0 64,977 + BT0066 0.59 +0.3 64,978 - BT0066 0.59 +0.8 65,023 + BT0066 0.62 -2.4
Or see this region's nucleotide sequence