Experiment: MoLS4 with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVUA0010 and nifK are separated by 190 nucleotides nifK and nifD are separated by 36 nucleotides
DVUA0010: DVUA0010 - ferredoxin, 2fe-2s (TIGR), at 14,308 to 14,610
DVUA0010
DVUA0011: nifK - nitrogenase molybdenum-iron protein beta chain (TIGR), at 14,801 to 16,186
nifK
DVUA0012: nifD - nitrogenase molybdenum-iron protein alpha chain (TIGR), at 16,223 to 17,851
nifD
Position (kb) 14
15
16
17 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 13.819 kb on + strand at 13.826 kb on + strand at 13.863 kb on - strand at 13.900 kb on + strand at 13.939 kb on + strand at 13.944 kb on + strand at 13.947 kb on - strand at 13.947 kb on - strand at 13.947 kb on - strand at 13.958 kb on + strand at 14.001 kb on + strand at 14.023 kb on + strand at 14.045 kb on - strand at 14.069 kb on + strand at 14.069 kb on + strand at 14.077 kb on - strand at 14.077 kb on - strand at 14.082 kb on - strand at 14.082 kb on - strand at 14.082 kb on - strand at 14.106 kb on + strand at 14.109 kb on - strand at 14.325 kb on - strand at 14.330 kb on - strand at 14.509 kb on - strand, within DVUA0010 at 14.600 kb on + strand at 14.619 kb on + strand at 14.659 kb on - strand at 14.665 kb on + strand at 14.720 kb on - strand at 14.732 kb on + strand at 14.754 kb on + strand at 14.766 kb on + strand at 14.822 kb on + strand at 14.905 kb on - strand at 14.936 kb on + strand at 14.981 kb on - strand, within nifK at 15.033 kb on + strand, within nifK at 15.128 kb on - strand, within nifK at 15.130 kb on + strand, within nifK at 15.138 kb on + strand, within nifK at 15.166 kb on - strand, within nifK at 15.249 kb on + strand, within nifK at 15.336 kb on - strand, within nifK at 15.338 kb on + strand, within nifK at 15.348 kb on - strand, within nifK at 15.450 kb on - strand, within nifK at 15.533 kb on - strand, within nifK at 15.553 kb on - strand, within nifK at 15.565 kb on + strand, within nifK at 15.583 kb on + strand, within nifK at 15.593 kb on + strand, within nifK at 15.601 kb on - strand, within nifK at 15.634 kb on + strand, within nifK at 15.691 kb on - strand, within nifK at 15.702 kb on + strand, within nifK at 15.749 kb on + strand, within nifK at 15.789 kb on + strand, within nifK at 16.041 kb on + strand, within nifK at 16.046 kb on + strand, within nifK at 16.152 kb on - strand at 16.163 kb on + strand at 16.210 kb on + strand at 16.216 kb on - strand at 16.265 kb on + strand at 16.281 kb on - strand at 16.345 kb on - strand at 16.354 kb on - strand at 16.631 kb on - strand, within nifD at 16.632 kb on - strand, within nifD at 16.677 kb on + strand, within nifD at 16.695 kb on - strand, within nifD at 16.732 kb on + strand, within nifD at 16.761 kb on + strand, within nifD at 16.856 kb on - strand, within nifD at 16.856 kb on - strand, within nifD at 16.856 kb on - strand, within nifD at 16.866 kb on - strand, within nifD at 16.920 kb on + strand, within nifD at 16.923 kb on - strand, within nifD at 16.928 kb on - strand, within nifD at 16.994 kb on - strand, within nifD at 17.040 kb on + strand, within nifD at 17.064 kb on + strand, within nifD at 17.072 kb on - strand, within nifD at 17.100 kb on + strand, within nifD at 17.131 kb on - strand, within nifD at 17.173 kb on - strand, within nifD
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with Casaminos 0.2% remove 13,819 + -0.1 13,826 + -0.7 13,863 - -0.1 13,900 + -0.1 13,939 + +0.1 13,944 + -1.3 13,947 - +0.1 13,947 - -0.8 13,947 - -0.1 13,958 + +0.3 14,001 + -0.1 14,023 + -1.1 14,045 - -0.2 14,069 + +0.7 14,069 + -0.0 14,077 - -0.2 14,077 - +0.1 14,082 - +0.7 14,082 - -0.5 14,082 - -0.4 14,106 + +0.0 14,109 - -0.1 14,325 - +0.2 14,330 - +0.1 14,509 - DVUA0010 0.66 -1.0 14,600 + -0.1 14,619 + -0.1 14,659 - +1.2 14,665 + +0.1 14,720 - -1.0 14,732 + -0.0 14,754 + -0.7 14,766 + -0.9 14,822 + -0.4 14,905 - -0.2 14,936 + -0.5 14,981 - nifK DVUA0011 0.13 -0.0 15,033 + nifK DVUA0011 0.17 +0.1 15,128 - nifK DVUA0011 0.24 -0.4 15,130 + nifK DVUA0011 0.24 -1.7 15,138 + nifK DVUA0011 0.24 +0.4 15,166 - nifK DVUA0011 0.26 +0.3 15,249 + nifK DVUA0011 0.32 +0.1 15,336 - nifK DVUA0011 0.39 -0.2 15,338 + nifK DVUA0011 0.39 +0.9 15,348 - nifK DVUA0011 0.39 -0.2 15,450 - nifK DVUA0011 0.47 +0.7 15,533 - nifK DVUA0011 0.53 -2.8 15,553 - nifK DVUA0011 0.54 -0.2 15,565 + nifK DVUA0011 0.55 -0.2 15,583 + nifK DVUA0011 0.56 +0.1 15,593 + nifK DVUA0011 0.57 -0.6 15,601 - nifK DVUA0011 0.58 +0.7 15,634 + nifK DVUA0011 0.60 +0.3 15,691 - nifK DVUA0011 0.64 -0.2 15,702 + nifK DVUA0011 0.65 -0.4 15,749 + nifK DVUA0011 0.68 -0.8 15,789 + nifK DVUA0011 0.71 -0.0 16,041 + nifK DVUA0011 0.89 +0.6 16,046 + nifK DVUA0011 0.90 -0.9 16,152 - +0.3 16,163 + -1.0 16,210 + +0.0 16,216 - -0.0 16,265 + -1.7 16,281 - -1.1 16,345 - -0.2 16,354 - +0.0 16,631 - nifD DVUA0012 0.25 +0.1 16,632 - nifD DVUA0012 0.25 -0.3 16,677 + nifD DVUA0012 0.28 +0.0 16,695 - nifD DVUA0012 0.29 +0.0 16,732 + nifD DVUA0012 0.31 +0.0 16,761 + nifD DVUA0012 0.33 -0.8 16,856 - nifD DVUA0012 0.39 -0.8 16,856 - nifD DVUA0012 0.39 -2.3 16,856 - nifD DVUA0012 0.39 -1.9 16,866 - nifD DVUA0012 0.39 -0.2 16,920 + nifD DVUA0012 0.43 -0.3 16,923 - nifD DVUA0012 0.43 +0.0 16,928 - nifD DVUA0012 0.43 +0.7 16,994 - nifD DVUA0012 0.47 +0.2 17,040 + nifD DVUA0012 0.50 +0.9 17,064 + nifD DVUA0012 0.52 -0.6 17,072 - nifD DVUA0012 0.52 -0.7 17,100 + nifD DVUA0012 0.54 -1.4 17,131 - nifD DVUA0012 0.56 -0.1 17,173 - nifD DVUA0012 0.58 -2.0
Or see this region's nucleotide sequence