Experiment: MoLS4 with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2490 and DVU2491 are separated by 5 nucleotides DVU2491 and trpF-2 are separated by 85 nucleotides
DVU2490: DVU2490 - Histidinol phosphatase (Natalia Ivanova), at 2,601,007 to 2,601,831
DVU2490
DVU2491: DVU2491 - ABC transporter, ATP-binding protein (TIGR), at 2,601,837 to 2,603,735
DVU2491
DVU2492: trpF-2 - N-(5-phosphoribosyl)anthranilate isomerase (TIGR), at 2,603,821 to 2,604,747
trpF-2
Position (kb)
2601
2602
2603
2604 Strain fitness (log2 ratio)
-2
-1
0
1 at 2600.843 kb on + strand at 2600.848 kb on + strand at 2600.851 kb on - strand at 2600.922 kb on - strand at 2600.969 kb on - strand at 2600.979 kb on + strand at 2601.927 kb on - strand at 2601.936 kb on + strand at 2601.992 kb on + strand at 2602.079 kb on - strand, within DVU2491 at 2602.081 kb on + strand, within DVU2491 at 2602.104 kb on + strand, within DVU2491 at 2602.223 kb on + strand, within DVU2491 at 2602.250 kb on + strand, within DVU2491 at 2602.343 kb on + strand, within DVU2491 at 2602.389 kb on + strand, within DVU2491 at 2602.389 kb on + strand, within DVU2491 at 2602.458 kb on + strand, within DVU2491 at 2602.467 kb on + strand, within DVU2491 at 2602.485 kb on - strand, within DVU2491 at 2602.487 kb on - strand, within DVU2491 at 2602.647 kb on - strand, within DVU2491 at 2602.699 kb on + strand, within DVU2491 at 2602.707 kb on - strand, within DVU2491 at 2602.714 kb on - strand, within DVU2491 at 2602.735 kb on + strand, within DVU2491 at 2602.735 kb on + strand, within DVU2491 at 2602.756 kb on + strand, within DVU2491 at 2602.761 kb on + strand, within DVU2491 at 2602.769 kb on - strand, within DVU2491 at 2602.814 kb on + strand, within DVU2491 at 2602.897 kb on - strand, within DVU2491 at 2602.918 kb on + strand, within DVU2491 at 2602.921 kb on - strand, within DVU2491 at 2603.021 kb on + strand, within DVU2491 at 2603.058 kb on + strand, within DVU2491 at 2603.072 kb on + strand, within DVU2491 at 2603.076 kb on - strand, within DVU2491 at 2603.199 kb on + strand, within DVU2491 at 2603.322 kb on + strand, within DVU2491 at 2603.323 kb on - strand, within DVU2491 at 2603.323 kb on - strand, within DVU2491 at 2603.335 kb on + strand, within DVU2491 at 2603.387 kb on - strand, within DVU2491 at 2603.387 kb on - strand, within DVU2491 at 2603.429 kb on + strand, within DVU2491 at 2603.448 kb on + strand, within DVU2491 at 2603.545 kb on + strand, within DVU2491 at 2603.553 kb on - strand at 2603.563 kb on - strand at 2603.597 kb on - strand at 2603.610 kb on - strand at 2603.694 kb on - strand at 2603.931 kb on + strand, within trpF-2 at 2603.946 kb on - strand, within trpF-2 at 2604.045 kb on + strand, within trpF-2 at 2604.144 kb on - strand, within trpF-2 at 2604.213 kb on - strand, within trpF-2 at 2604.232 kb on + strand, within trpF-2 at 2604.270 kb on + strand, within trpF-2 at 2604.275 kb on + strand, within trpF-2 at 2604.288 kb on - strand, within trpF-2 at 2604.377 kb on + strand, within trpF-2 at 2604.377 kb on + strand, within trpF-2 at 2604.385 kb on - strand, within trpF-2 at 2604.385 kb on - strand, within trpF-2 at 2604.385 kb on - strand, within trpF-2 at 2604.385 kb on - strand, within trpF-2 at 2604.385 kb on - strand, within trpF-2 at 2604.431 kb on - strand, within trpF-2 at 2604.460 kb on + strand, within trpF-2 at 2604.530 kb on - strand, within trpF-2 at 2604.541 kb on + strand, within trpF-2 at 2604.621 kb on + strand, within trpF-2 at 2604.662 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with Casaminos 0.2% remove 2,600,843 + -0.5 2,600,848 + +0.0 2,600,851 - +0.3 2,600,922 - -0.1 2,600,969 - -0.8 2,600,979 + -0.1 2,601,927 - -1.0 2,601,936 + -0.2 2,601,992 + +1.3 2,602,079 - DVU2491 0.13 -1.4 2,602,081 + DVU2491 0.13 -1.8 2,602,104 + DVU2491 0.14 -0.5 2,602,223 + DVU2491 0.20 -0.1 2,602,250 + DVU2491 0.22 -0.1 2,602,343 + DVU2491 0.27 +0.4 2,602,389 + DVU2491 0.29 -0.1 2,602,389 + DVU2491 0.29 +0.1 2,602,458 + DVU2491 0.33 -0.1 2,602,467 + DVU2491 0.33 -1.0 2,602,485 - DVU2491 0.34 -0.0 2,602,487 - DVU2491 0.34 -1.6 2,602,647 - DVU2491 0.43 -0.4 2,602,699 + DVU2491 0.45 -0.3 2,602,707 - DVU2491 0.46 -0.3 2,602,714 - DVU2491 0.46 -0.3 2,602,735 + DVU2491 0.47 -0.2 2,602,735 + DVU2491 0.47 -0.2 2,602,756 + DVU2491 0.48 +0.5 2,602,761 + DVU2491 0.49 -1.3 2,602,769 - DVU2491 0.49 -0.5 2,602,814 + DVU2491 0.51 -1.0 2,602,897 - DVU2491 0.56 -0.0 2,602,918 + DVU2491 0.57 -0.1 2,602,921 - DVU2491 0.57 -2.2 2,603,021 + DVU2491 0.62 -0.7 2,603,058 + DVU2491 0.64 +0.3 2,603,072 + DVU2491 0.65 -0.3 2,603,076 - DVU2491 0.65 -0.1 2,603,199 + DVU2491 0.72 -2.4 2,603,322 + DVU2491 0.78 -0.5 2,603,323 - DVU2491 0.78 -0.3 2,603,323 - DVU2491 0.78 -0.0 2,603,335 + DVU2491 0.79 -1.4 2,603,387 - DVU2491 0.82 -1.0 2,603,387 - DVU2491 0.82 -0.3 2,603,429 + DVU2491 0.84 +0.2 2,603,448 + DVU2491 0.85 -0.3 2,603,545 + DVU2491 0.90 -1.1 2,603,553 - -0.3 2,603,563 - -0.5 2,603,597 - -0.0 2,603,610 - +0.5 2,603,694 - -0.6 2,603,931 + trpF-2 DVU2492 0.12 +0.1 2,603,946 - trpF-2 DVU2492 0.13 -0.6 2,604,045 + trpF-2 DVU2492 0.24 -0.0 2,604,144 - trpF-2 DVU2492 0.35 -0.1 2,604,213 - trpF-2 DVU2492 0.42 -0.4 2,604,232 + trpF-2 DVU2492 0.44 -0.5 2,604,270 + trpF-2 DVU2492 0.48 -1.1 2,604,275 + trpF-2 DVU2492 0.49 -0.2 2,604,288 - trpF-2 DVU2492 0.50 -0.4 2,604,377 + trpF-2 DVU2492 0.60 +0.4 2,604,377 + trpF-2 DVU2492 0.60 -0.1 2,604,385 - trpF-2 DVU2492 0.61 -0.4 2,604,385 - trpF-2 DVU2492 0.61 +0.4 2,604,385 - trpF-2 DVU2492 0.61 +0.3 2,604,385 - trpF-2 DVU2492 0.61 -0.3 2,604,385 - trpF-2 DVU2492 0.61 -0.4 2,604,431 - trpF-2 DVU2492 0.66 +0.6 2,604,460 + trpF-2 DVU2492 0.69 +0.6 2,604,530 - trpF-2 DVU2492 0.76 +0.4 2,604,541 + trpF-2 DVU2492 0.78 -0.4 2,604,621 + trpF-2 DVU2492 0.86 +0.5 2,604,662 + -0.4
Or see this region's nucleotide sequence