Experiment: MoLS4 with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ccmG and DVU1587 overlap by 1 nucleotides DVU1587 and hpt are separated by 80 nucleotides hpt and DVU1589 are separated by 30 nucleotides DVU1589 and radA are separated by 12 nucleotides
DVU1586: ccmG - thioredoxin family protein (TIGR), at 1,669,924 to 1,670,391
ccmG
DVU1587: DVU1587 - N-acetylglutamate synthase (EC 2.3.1.1) (from data) , at 1,670,391 to 1,670,855
DVU1587
DVU1588: hpt - hypoxanthine phosphoribosyltransferase (TIGR), at 1,670,936 to 1,671,466
hpt
DVU1589: DVU1589 - hypothetical protein (TIGR), at 1,671,497 to 1,672,285
DVU1589
DVU1590: radA - DNA repair protein RadA (TIGR), at 1,672,298 to 1,673,668
radA
Position (kb)
1670
1671
1672 Strain fitness (log2 ratio)
-1
0
1 at 1669.974 kb on - strand, within ccmG at 1669.974 kb on - strand, within ccmG at 1670.079 kb on - strand, within ccmG at 1670.098 kb on - strand, within ccmG at 1670.130 kb on + strand, within ccmG at 1670.212 kb on + strand, within ccmG at 1670.443 kb on - strand, within DVU1587 at 1670.456 kb on - strand, within DVU1587 at 1670.480 kb on + strand, within DVU1587 at 1670.563 kb on + strand, within DVU1587 at 1670.571 kb on - strand, within DVU1587 at 1670.634 kb on + strand, within DVU1587 at 1670.653 kb on + strand, within DVU1587 at 1670.783 kb on + strand, within DVU1587 at 1670.783 kb on + strand, within DVU1587 at 1670.784 kb on - strand, within DVU1587 at 1670.852 kb on + strand at 1670.917 kb on + strand at 1670.950 kb on + strand at 1670.950 kb on + strand at 1670.958 kb on - strand at 1670.958 kb on - strand at 1670.975 kb on - strand at 1671.037 kb on - strand, within hpt at 1671.259 kb on - strand, within hpt at 1671.419 kb on - strand at 1671.527 kb on + strand at 1671.571 kb on - strand at 1671.595 kb on + strand, within DVU1589 at 1671.712 kb on - strand, within DVU1589 at 1671.848 kb on + strand, within DVU1589 at 1671.919 kb on + strand, within DVU1589 at 1671.966 kb on + strand, within DVU1589 at 1672.063 kb on + strand, within DVU1589 at 1672.071 kb on + strand, within DVU1589 at 1672.101 kb on - strand, within DVU1589 at 1672.241 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with Casaminos 0.2% remove 1,669,974 - ccmG DVU1586 0.11 -1.0 1,669,974 - ccmG DVU1586 0.11 -0.5 1,670,079 - ccmG DVU1586 0.33 -0.2 1,670,098 - ccmG DVU1586 0.37 -0.3 1,670,130 + ccmG DVU1586 0.44 -0.0 1,670,212 + ccmG DVU1586 0.62 -0.4 1,670,443 - DVU1587 0.11 +0.4 1,670,456 - DVU1587 0.14 -0.1 1,670,480 + DVU1587 0.19 -0.7 1,670,563 + DVU1587 0.37 -0.1 1,670,571 - DVU1587 0.39 +0.3 1,670,634 + DVU1587 0.52 -0.8 1,670,653 + DVU1587 0.56 -0.1 1,670,783 + DVU1587 0.84 -0.3 1,670,783 + DVU1587 0.84 -0.2 1,670,784 - DVU1587 0.85 -0.1 1,670,852 + -0.4 1,670,917 + -0.3 1,670,950 + +0.1 1,670,950 + -0.3 1,670,958 - +0.3 1,670,958 - +0.3 1,670,975 - -0.6 1,671,037 - hpt DVU1588 0.19 -0.1 1,671,259 - hpt DVU1588 0.61 -0.2 1,671,419 - +0.6 1,671,527 + +0.3 1,671,571 - +1.2 1,671,595 + DVU1589 0.12 +0.0 1,671,712 - DVU1589 0.27 -0.5 1,671,848 + DVU1589 0.44 +0.2 1,671,919 + DVU1589 0.53 +0.4 1,671,966 + DVU1589 0.59 +0.3 1,672,063 + DVU1589 0.72 +0.1 1,672,071 + DVU1589 0.73 +0.2 1,672,101 - DVU1589 0.77 -1.7 1,672,241 - +0.0
Or see this region's nucleotide sequence