Experiment: PYE with nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02607 and CCNA_02608 are separated by 155 nucleotides CCNA_02608 and CCNA_02609 are separated by 10 nucleotides CCNA_02609 and CCNA_02610 are separated by 32 nucleotides
CCNA_02607: CCNA_02607 - isocitrate dehydrogenase, at 2,753,922 to 2,755,133
_02607
CCNA_02608: CCNA_02608 - hypothetical protein, at 2,755,289 to 2,755,867
_02608
CCNA_02609: CCNA_02609 - membrane transport protein, at 2,755,878 to 2,756,816
_02609
CCNA_02610: CCNA_02610 - glyoxalase family protein, at 2,756,849 to 2,757,226
_02610
Position (kb)
2755
2756 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2755.121 kb on + strand at 2755.128 kb on + strand at 2755.128 kb on + strand at 2755.136 kb on + strand at 2755.136 kb on + strand at 2755.214 kb on + strand at 2755.214 kb on + strand at 2755.245 kb on + strand at 2755.250 kb on + strand at 2755.253 kb on - strand at 2755.258 kb on - strand at 2755.287 kb on + strand at 2755.293 kb on - strand at 2755.308 kb on + strand at 2755.402 kb on + strand, within CCNA_02608 at 2755.402 kb on + strand, within CCNA_02608 at 2755.402 kb on + strand, within CCNA_02608 at 2755.438 kb on + strand, within CCNA_02608 at 2755.438 kb on + strand, within CCNA_02608 at 2755.446 kb on - strand, within CCNA_02608 at 2755.474 kb on + strand, within CCNA_02608 at 2755.573 kb on + strand, within CCNA_02608 at 2755.575 kb on - strand, within CCNA_02608 at 2755.621 kb on - strand, within CCNA_02608 at 2755.626 kb on + strand, within CCNA_02608 at 2755.646 kb on - strand, within CCNA_02608 at 2755.679 kb on + strand, within CCNA_02608 at 2755.679 kb on + strand, within CCNA_02608 at 2755.693 kb on - strand, within CCNA_02608 at 2755.694 kb on + strand, within CCNA_02608 at 2755.780 kb on + strand, within CCNA_02608 at 2755.787 kb on + strand, within CCNA_02608 at 2755.787 kb on + strand, within CCNA_02608 at 2755.821 kb on - strand at 2755.885 kb on + strand at 2755.949 kb on - strand at 2755.949 kb on - strand at 2755.986 kb on - strand, within CCNA_02609 at 2755.992 kb on + strand, within CCNA_02609 at 2756.012 kb on + strand, within CCNA_02609 at 2756.030 kb on - strand, within CCNA_02609 at 2756.043 kb on + strand, within CCNA_02609 at 2756.048 kb on + strand, within CCNA_02609 at 2756.059 kb on + strand, within CCNA_02609 at 2756.083 kb on - strand, within CCNA_02609 at 2756.144 kb on + strand, within CCNA_02609 at 2756.145 kb on + strand, within CCNA_02609 at 2756.145 kb on + strand, within CCNA_02609 at 2756.155 kb on + strand, within CCNA_02609 at 2756.163 kb on - strand, within CCNA_02609 at 2756.163 kb on - strand, within CCNA_02609 at 2756.163 kb on - strand, within CCNA_02609 at 2756.163 kb on - strand, within CCNA_02609 at 2756.165 kb on + strand, within CCNA_02609 at 2756.173 kb on - strand, within CCNA_02609 at 2756.200 kb on - strand, within CCNA_02609 at 2756.313 kb on + strand, within CCNA_02609 at 2756.321 kb on - strand, within CCNA_02609 at 2756.363 kb on - strand, within CCNA_02609 at 2756.429 kb on + strand, within CCNA_02609 at 2756.437 kb on - strand, within CCNA_02609 at 2756.437 kb on - strand, within CCNA_02609 at 2756.474 kb on - strand, within CCNA_02609 at 2756.513 kb on - strand, within CCNA_02609 at 2756.516 kb on + strand, within CCNA_02609 at 2756.516 kb on + strand, within CCNA_02609 at 2756.554 kb on + strand, within CCNA_02609 at 2756.567 kb on - strand, within CCNA_02609 at 2756.573 kb on + strand, within CCNA_02609 at 2756.579 kb on + strand, within CCNA_02609 at 2756.580 kb on + strand, within CCNA_02609 at 2756.587 kb on - strand, within CCNA_02609 at 2756.590 kb on + strand, within CCNA_02609 at 2756.595 kb on + strand, within CCNA_02609 at 2756.598 kb on - strand, within CCNA_02609 at 2756.608 kb on - strand, within CCNA_02609 at 2756.617 kb on + strand, within CCNA_02609 at 2756.694 kb on + strand, within CCNA_02609 at 2756.694 kb on + strand, within CCNA_02609 at 2756.694 kb on + strand, within CCNA_02609 at 2756.702 kb on - strand, within CCNA_02609 at 2756.705 kb on + strand, within CCNA_02609 at 2756.713 kb on + strand, within CCNA_02609 at 2756.713 kb on - strand, within CCNA_02609 at 2756.721 kb on - strand, within CCNA_02609 at 2756.723 kb on - strand at 2756.772 kb on - strand at 2756.802 kb on + strand at 2756.826 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrate 100 mM remove 2,755,121 + +2.5 2,755,128 + +2.8 2,755,128 + +2.7 2,755,136 + +2.5 2,755,136 + +2.8 2,755,214 + +2.7 2,755,214 + -0.6 2,755,245 + +1.8 2,755,250 + -1.0 2,755,253 - +1.0 2,755,258 - -0.4 2,755,287 + +1.0 2,755,293 - +0.3 2,755,308 + -1.8 2,755,402 + CCNA_02608 0.20 +1.4 2,755,402 + CCNA_02608 0.20 -2.1 2,755,402 + CCNA_02608 0.20 +0.4 2,755,438 + CCNA_02608 0.26 +2.6 2,755,438 + CCNA_02608 0.26 +2.0 2,755,446 - CCNA_02608 0.27 -0.0 2,755,474 + CCNA_02608 0.32 +0.5 2,755,573 + CCNA_02608 0.49 +0.4 2,755,575 - CCNA_02608 0.49 -0.2 2,755,621 - CCNA_02608 0.57 -0.7 2,755,626 + CCNA_02608 0.58 +1.8 2,755,646 - CCNA_02608 0.62 -0.2 2,755,679 + CCNA_02608 0.67 +1.3 2,755,679 + CCNA_02608 0.67 +1.1 2,755,693 - CCNA_02608 0.70 +1.0 2,755,694 + CCNA_02608 0.70 +0.3 2,755,780 + CCNA_02608 0.85 +1.7 2,755,787 + CCNA_02608 0.86 +1.0 2,755,787 + CCNA_02608 0.86 -0.3 2,755,821 - +0.3 2,755,885 + +1.3 2,755,949 - +0.8 2,755,949 - -2.1 2,755,986 - CCNA_02609 0.12 +0.8 2,755,992 + CCNA_02609 0.12 +1.3 2,756,012 + CCNA_02609 0.14 +1.5 2,756,030 - CCNA_02609 0.16 +0.7 2,756,043 + CCNA_02609 0.18 +2.3 2,756,048 + CCNA_02609 0.18 +0.4 2,756,059 + CCNA_02609 0.19 +0.9 2,756,083 - CCNA_02609 0.22 -2.4 2,756,144 + CCNA_02609 0.28 +0.4 2,756,145 + CCNA_02609 0.28 -1.4 2,756,145 + CCNA_02609 0.28 +1.5 2,756,155 + CCNA_02609 0.29 +1.3 2,756,163 - CCNA_02609 0.30 -1.7 2,756,163 - CCNA_02609 0.30 -0.6 2,756,163 - CCNA_02609 0.30 -0.1 2,756,163 - CCNA_02609 0.30 -0.1 2,756,165 + CCNA_02609 0.31 +2.0 2,756,173 - CCNA_02609 0.31 -0.6 2,756,200 - CCNA_02609 0.34 +0.6 2,756,313 + CCNA_02609 0.46 +1.4 2,756,321 - CCNA_02609 0.47 +0.9 2,756,363 - CCNA_02609 0.52 +0.9 2,756,429 + CCNA_02609 0.59 +0.9 2,756,437 - CCNA_02609 0.60 -0.4 2,756,437 - CCNA_02609 0.60 +0.7 2,756,474 - CCNA_02609 0.63 -0.6 2,756,513 - CCNA_02609 0.68 -0.1 2,756,516 + CCNA_02609 0.68 +0.7 2,756,516 + CCNA_02609 0.68 +0.2 2,756,554 + CCNA_02609 0.72 +1.8 2,756,567 - CCNA_02609 0.73 -0.1 2,756,573 + CCNA_02609 0.74 +0.1 2,756,579 + CCNA_02609 0.75 -1.6 2,756,580 + CCNA_02609 0.75 +3.3 2,756,587 - CCNA_02609 0.76 +1.3 2,756,590 + CCNA_02609 0.76 +0.0 2,756,595 + CCNA_02609 0.76 -0.1 2,756,598 - CCNA_02609 0.77 +1.7 2,756,608 - CCNA_02609 0.78 +0.8 2,756,617 + CCNA_02609 0.79 -0.6 2,756,694 + CCNA_02609 0.87 +0.7 2,756,694 + CCNA_02609 0.87 +1.2 2,756,694 + CCNA_02609 0.87 +0.1 2,756,702 - CCNA_02609 0.88 +1.5 2,756,705 + CCNA_02609 0.88 +0.4 2,756,713 + CCNA_02609 0.89 +0.1 2,756,713 - CCNA_02609 0.89 -0.4 2,756,721 - CCNA_02609 0.90 -1.5 2,756,723 - +1.1 2,756,772 - +0.7 2,756,802 + -1.7 2,756,826 - -0.1
Or see this region's nucleotide sequence