Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00498

Experiment: PYE with nitrate 100 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00496 and CCNA_00497 overlap by 1 nucleotidesCCNA_00497 and CCNA_00498 are separated by 60 nucleotidesCCNA_00498 and CCNA_00499 are separated by 75 nucleotidesCCNA_00499 and CCNA_00500 overlap by 4 nucleotides CCNA_00496: CCNA_00496 - threonyl-tRNA synthetase, at 512,331 to 514,298 _00496 CCNA_00497: CCNA_00497 - putative rhamnosyl transferase, at 514,298 to 515,224 _00497 CCNA_00498: CCNA_00498 - hypothetical protein, at 515,285 to 515,404 _00498 CCNA_00499: CCNA_00499 - hypothetical protein, at 515,480 to 515,845 _00499 CCNA_00500: CCNA_00500 - hydroxymethylglutaryl-CoA lyase, at 515,842 to 516,741 _00500 Position (kb) 515 516Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 514.305 kb on + strandat 514.310 kb on + strandat 514.313 kb on - strandat 514.319 kb on + strandat 514.351 kb on - strandat 514.352 kb on + strandat 514.353 kb on + strandat 514.362 kb on + strandat 514.402 kb on + strand, within CCNA_00497at 514.402 kb on + strand, within CCNA_00497at 514.410 kb on - strand, within CCNA_00497at 514.465 kb on - strand, within CCNA_00497at 514.512 kb on - strand, within CCNA_00497at 514.555 kb on - strand, within CCNA_00497at 514.574 kb on - strand, within CCNA_00497at 514.608 kb on - strandat 514.617 kb on + strand, within CCNA_00497at 514.636 kb on + strand, within CCNA_00497at 514.638 kb on - strand, within CCNA_00497at 514.642 kb on - strand, within CCNA_00497at 514.644 kb on - strand, within CCNA_00497at 514.657 kb on + strand, within CCNA_00497at 514.664 kb on + strand, within CCNA_00497at 514.681 kb on - strand, within CCNA_00497at 514.706 kb on + strand, within CCNA_00497at 514.706 kb on + strand, within CCNA_00497at 514.748 kb on + strand, within CCNA_00497at 514.753 kb on + strand, within CCNA_00497at 514.756 kb on - strand, within CCNA_00497at 514.757 kb on + strand, within CCNA_00497at 514.762 kb on + strand, within CCNA_00497at 514.767 kb on + strand, within CCNA_00497at 514.770 kb on - strand, within CCNA_00497at 514.772 kb on + strand, within CCNA_00497at 514.772 kb on + strand, within CCNA_00497at 514.772 kb on + strand, within CCNA_00497at 514.772 kb on + strand, within CCNA_00497at 514.775 kb on - strand, within CCNA_00497at 514.780 kb on - strand, within CCNA_00497at 514.780 kb on - strand, within CCNA_00497at 514.780 kb on - strand, within CCNA_00497at 514.787 kb on - strand, within CCNA_00497at 514.803 kb on + strand, within CCNA_00497at 514.803 kb on + strand, within CCNA_00497at 514.803 kb on + strand, within CCNA_00497at 514.811 kb on - strand, within CCNA_00497at 514.811 kb on - strand, within CCNA_00497at 514.813 kb on + strand, within CCNA_00497at 514.818 kb on + strand, within CCNA_00497at 514.852 kb on - strand, within CCNA_00497at 514.937 kb on - strand, within CCNA_00497at 514.980 kb on - strand, within CCNA_00497at 514.998 kb on + strand, within CCNA_00497at 515.003 kb on + strand, within CCNA_00497at 515.003 kb on + strand, within CCNA_00497at 515.008 kb on + strand, within CCNA_00497at 515.011 kb on - strand, within CCNA_00497at 515.014 kb on + strand, within CCNA_00497at 515.028 kb on - strand, within CCNA_00497at 515.039 kb on + strand, within CCNA_00497at 515.047 kb on - strand, within CCNA_00497at 515.049 kb on + strand, within CCNA_00497at 515.049 kb on + strand, within CCNA_00497at 515.057 kb on - strand, within CCNA_00497at 515.077 kb on + strand, within CCNA_00497at 515.077 kb on - strand, within CCNA_00497at 515.084 kb on + strand, within CCNA_00497at 515.085 kb on - strand, within CCNA_00497at 515.090 kb on + strand, within CCNA_00497at 515.090 kb on + strand, within CCNA_00497at 515.090 kb on + strand, within CCNA_00497at 515.155 kb on - strandat 515.220 kb on + strandat 515.230 kb on + strandat 515.231 kb on + strandat 515.238 kb on - strandat 515.257 kb on - strandat 515.277 kb on + strandat 515.290 kb on - strandat 515.297 kb on + strand, within CCNA_00498at 515.300 kb on - strand, within CCNA_00498at 515.349 kb on + strand, within CCNA_00498at 515.349 kb on + strand, within CCNA_00498at 515.357 kb on - strand, within CCNA_00498at 515.368 kb on + strand, within CCNA_00498at 515.374 kb on + strand, within CCNA_00498at 515.382 kb on - strand, within CCNA_00498at 515.443 kb on - strandat 515.465 kb on + strandat 515.538 kb on + strand, within CCNA_00499at 515.546 kb on - strand, within CCNA_00499at 515.565 kb on + strand, within CCNA_00499at 515.565 kb on + strand, within CCNA_00499at 515.582 kb on - strand, within CCNA_00499at 515.586 kb on + strand, within CCNA_00499at 515.604 kb on + strand, within CCNA_00499at 515.640 kb on - strand, within CCNA_00499at 515.642 kb on + strand, within CCNA_00499at 515.642 kb on + strand, within CCNA_00499at 515.642 kb on + strand, within CCNA_00499at 515.689 kb on - strand, within CCNA_00499at 515.713 kb on + strand, within CCNA_00499at 515.713 kb on + strand, within CCNA_00499at 515.721 kb on - strand, within CCNA_00499at 515.767 kb on + strand, within CCNA_00499at 515.820 kb on - strandat 515.846 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with nitrate 100 mM
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514,305 + +2.2
514,310 + +1.6
514,313 - -1.0
514,319 + +1.6
514,351 - +0.1
514,352 + +2.1
514,353 + +2.2
514,362 + +1.8
514,402 + CCNA_00497 0.11 +1.6
514,402 + CCNA_00497 0.11 +1.3
514,410 - CCNA_00497 0.12 +1.0
514,465 - CCNA_00497 0.18 +1.1
514,512 - CCNA_00497 0.23 -0.0
514,555 - CCNA_00497 0.28 -1.9
514,574 - CCNA_00497 0.30 -0.4
514,608 - +2.1
514,617 + CCNA_00497 0.34 +3.1
514,636 + CCNA_00497 0.36 +1.4
514,638 - CCNA_00497 0.37 +1.7
514,642 - CCNA_00497 0.37 -1.1
514,644 - CCNA_00497 0.37 -1.0
514,657 + CCNA_00497 0.39 +1.6
514,664 + CCNA_00497 0.39 +1.8
514,681 - CCNA_00497 0.41 -0.9
514,706 + CCNA_00497 0.44 +1.2
514,706 + CCNA_00497 0.44 +1.8
514,748 + CCNA_00497 0.49 +1.6
514,753 + CCNA_00497 0.49 +1.6
514,756 - CCNA_00497 0.49 +1.1
514,757 + CCNA_00497 0.50 +1.6
514,762 + CCNA_00497 0.50 +1.7
514,767 + CCNA_00497 0.51 +1.7
514,770 - CCNA_00497 0.51 +0.6
514,772 + CCNA_00497 0.51 +1.9
514,772 + CCNA_00497 0.51 +1.5
514,772 + CCNA_00497 0.51 +1.7
514,772 + CCNA_00497 0.51 +1.4
514,775 - CCNA_00497 0.51 -1.1
514,780 - CCNA_00497 0.52 -2.8
514,780 - CCNA_00497 0.52 -3.1
514,780 - CCNA_00497 0.52 -0.4
514,787 - CCNA_00497 0.53 -1.2
514,803 + CCNA_00497 0.54 +2.1
514,803 + CCNA_00497 0.54 +2.2
514,803 + CCNA_00497 0.54 +1.8
514,811 - CCNA_00497 0.55 +1.1
514,811 - CCNA_00497 0.55 +2.1
514,813 + CCNA_00497 0.56 +2.0
514,818 + CCNA_00497 0.56 +1.5
514,852 - CCNA_00497 0.60 -1.7
514,937 - CCNA_00497 0.69 -1.6
514,980 - CCNA_00497 0.74 -1.0
514,998 + CCNA_00497 0.76 +1.8
515,003 + CCNA_00497 0.76 +2.2
515,003 + CCNA_00497 0.76 +1.4
515,008 + CCNA_00497 0.77 +2.6
515,011 - CCNA_00497 0.77 +1.1
515,014 + CCNA_00497 0.77 +1.9
515,028 - CCNA_00497 0.79 +0.8
515,039 + CCNA_00497 0.80 +1.7
515,047 - CCNA_00497 0.81 +0.1
515,049 + CCNA_00497 0.81 +1.5
515,049 + CCNA_00497 0.81 +0.8
515,057 - CCNA_00497 0.82 +1.4
515,077 + CCNA_00497 0.84 +1.5
515,077 - CCNA_00497 0.84 +0.8
515,084 + CCNA_00497 0.85 +1.5
515,085 - CCNA_00497 0.85 -0.7
515,090 + CCNA_00497 0.85 +1.5
515,090 + CCNA_00497 0.85 +2.0
515,090 + CCNA_00497 0.85 +2.4
515,155 - -1.2
515,220 + +1.7
515,230 + +1.4
515,231 + +1.1
515,238 - +1.4
515,257 - +2.2
515,277 + +2.4
515,290 - -0.5
515,297 + CCNA_00498 0.10 +2.6
515,300 - CCNA_00498 0.12 -0.1
515,349 + CCNA_00498 0.53 +0.4
515,349 + CCNA_00498 0.53 +0.6
515,357 - CCNA_00498 0.60 -0.8
515,368 + CCNA_00498 0.69 +1.6
515,374 + CCNA_00498 0.74 +1.6
515,382 - CCNA_00498 0.81 +2.2
515,443 - +1.7
515,465 + +0.3
515,538 + CCNA_00499 0.16 +2.2
515,546 - CCNA_00499 0.18 -1.0
515,565 + CCNA_00499 0.23 +1.0
515,565 + CCNA_00499 0.23 +0.1
515,582 - CCNA_00499 0.28 +0.3
515,586 + CCNA_00499 0.29 +0.2
515,604 + CCNA_00499 0.34 +0.6
515,640 - CCNA_00499 0.44 +0.8
515,642 + CCNA_00499 0.44 +0.6
515,642 + CCNA_00499 0.44 +1.3
515,642 + CCNA_00499 0.44 -0.5
515,689 - CCNA_00499 0.57 +1.4
515,713 + CCNA_00499 0.64 +1.3
515,713 + CCNA_00499 0.64 -1.2
515,721 - CCNA_00499 0.66 -0.8
515,767 + CCNA_00499 0.78 -1.4
515,820 - +1.0
515,846 - +1.1

Or see this region's nucleotide sequence