Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13545 and BBR_RS13550 are separated by 108 nucleotides BBR_RS13550 and BBR_RS20780 overlap by 70 nucleotides BBR_RS20780 and BBR_RS13555 overlap by 2 nucleotides
BBR_RS13545: BBR_RS13545 - DUF4143 domain-containing protein, at 810,002 to 811,363
_RS13545
BBR_RS13550: BBR_RS13550 - DUF47 domain-containing protein, at 811,472 to 812,089
_RS13550
BBR_RS20780: BBR_RS20780 - hypothetical protein, at 812,020 to 812,304
_RS20780
BBR_RS13555: BBR_RS13555 - excinuclease ABC subunit A, at 812,303 to 814,807
_RS13555
Position (kb)
812
813 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 811.029 kb on - strand, within BBR_RS13545 at 811.114 kb on - strand, within BBR_RS13545 at 811.195 kb on - strand, within BBR_RS13545 at 811.196 kb on + strand, within BBR_RS13545 at 811.230 kb on + strand at 811.290 kb on - strand at 811.342 kb on + strand at 811.342 kb on + strand at 811.352 kb on - strand at 811.469 kb on + strand at 811.479 kb on - strand at 811.515 kb on - strand at 811.519 kb on + strand at 811.519 kb on + strand at 811.519 kb on + strand at 811.525 kb on - strand at 811.527 kb on - strand at 811.527 kb on - strand at 811.533 kb on - strand at 811.596 kb on + strand, within BBR_RS13550 at 811.613 kb on + strand, within BBR_RS13550 at 811.640 kb on + strand, within BBR_RS13550 at 811.675 kb on - strand, within BBR_RS13550 at 811.678 kb on + strand, within BBR_RS13550 at 811.678 kb on + strand, within BBR_RS13550 at 811.720 kb on + strand, within BBR_RS13550 at 811.736 kb on + strand, within BBR_RS13550 at 811.746 kb on + strand, within BBR_RS13550 at 811.759 kb on - strand, within BBR_RS13550 at 811.787 kb on - strand, within BBR_RS13550 at 811.811 kb on + strand, within BBR_RS13550 at 811.844 kb on + strand, within BBR_RS13550 at 811.859 kb on + strand, within BBR_RS13550 at 811.859 kb on + strand, within BBR_RS13550 at 811.859 kb on + strand, within BBR_RS13550 at 811.860 kb on - strand, within BBR_RS13550 at 811.865 kb on - strand, within BBR_RS13550 at 811.867 kb on - strand, within BBR_RS13550 at 811.880 kb on - strand, within BBR_RS13550 at 811.886 kb on - strand, within BBR_RS13550 at 811.965 kb on + strand, within BBR_RS13550 at 811.975 kb on + strand, within BBR_RS13550 at 811.981 kb on - strand, within BBR_RS13550 at 811.997 kb on + strand, within BBR_RS13550 at 812.002 kb on + strand, within BBR_RS13550 at 812.016 kb on + strand, within BBR_RS13550 at 812.028 kb on + strand at 812.177 kb on + strand, within BBR_RS20780 at 812.185 kb on + strand, within BBR_RS20780 at 812.226 kb on - strand, within BBR_RS20780 at 812.272 kb on + strand, within BBR_RS20780 at 812.275 kb on + strand, within BBR_RS20780 at 812.281 kb on - strand at 812.289 kb on + strand at 812.294 kb on + strand at 812.336 kb on - strand at 812.359 kb on + strand at 812.408 kb on + strand at 812.491 kb on + strand at 812.496 kb on + strand at 812.524 kb on - strand at 812.534 kb on - strand at 812.534 kb on - strand at 812.541 kb on + strand at 812.546 kb on + strand at 812.546 kb on + strand at 812.546 kb on + strand at 812.554 kb on - strand, within BBR_RS13555 at 812.573 kb on - strand, within BBR_RS13555 at 812.611 kb on - strand, within BBR_RS13555 at 812.632 kb on + strand, within BBR_RS13555 at 812.632 kb on + strand, within BBR_RS13555 at 812.632 kb on + strand, within BBR_RS13555 at 812.632 kb on + strand, within BBR_RS13555 at 812.640 kb on - strand, within BBR_RS13555 at 812.640 kb on - strand, within BBR_RS13555 at 812.640 kb on - strand, within BBR_RS13555 at 812.640 kb on - strand, within BBR_RS13555 at 812.640 kb on - strand, within BBR_RS13555 at 812.640 kb on - strand, within BBR_RS13555 at 812.655 kb on - strand, within BBR_RS13555 at 812.667 kb on + strand, within BBR_RS13555 at 812.731 kb on + strand, within BBR_RS13555 at 812.740 kb on - strand, within BBR_RS13555 at 812.740 kb on - strand, within BBR_RS13555 at 812.742 kb on + strand, within BBR_RS13555 at 812.745 kb on - strand, within BBR_RS13555 at 812.818 kb on - strand, within BBR_RS13555 at 812.839 kb on + strand, within BBR_RS13555 at 812.865 kb on + strand, within BBR_RS13555 at 812.930 kb on - strand, within BBR_RS13555 at 812.939 kb on - strand, within BBR_RS13555 at 812.944 kb on - strand, within BBR_RS13555 at 812.991 kb on + strand, within BBR_RS13555 at 812.991 kb on + strand, within BBR_RS13555 at 812.999 kb on - strand, within BBR_RS13555 at 813.094 kb on + strand, within BBR_RS13555 at 813.094 kb on + strand, within BBR_RS13555 at 813.094 kb on + strand, within BBR_RS13555 at 813.094 kb on + strand, within BBR_RS13555 at 813.101 kb on + strand, within BBR_RS13555 at 813.106 kb on + strand, within BBR_RS13555 at 813.109 kb on - strand, within BBR_RS13555 at 813.113 kb on + strand, within BBR_RS13555 at 813.118 kb on - strand, within BBR_RS13555 at 813.135 kb on - strand, within BBR_RS13555 at 813.149 kb on + strand, within BBR_RS13555 at 813.157 kb on - strand, within BBR_RS13555 at 813.157 kb on - strand, within BBR_RS13555 at 813.157 kb on - strand, within BBR_RS13555 at 813.231 kb on + strand, within BBR_RS13555 at 813.239 kb on - strand, within BBR_RS13555 at 813.243 kb on - strand, within BBR_RS13555 at 813.246 kb on + strand, within BBR_RS13555 at 813.246 kb on + strand, within BBR_RS13555 at 813.265 kb on - strand, within BBR_RS13555 at 813.277 kb on - strand, within BBR_RS13555
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 811,029 - BBR_RS13545 0.75 +0.2 811,114 - BBR_RS13545 0.82 -0.3 811,195 - BBR_RS13545 0.88 +0.6 811,196 + BBR_RS13545 0.88 +0.1 811,230 + +0.6 811,290 - -1.5 811,342 + -0.6 811,342 + -1.6 811,352 - +0.7 811,469 + +0.2 811,479 - +0.1 811,515 - +0.2 811,519 + +0.5 811,519 + -0.3 811,519 + -0.3 811,525 - +0.0 811,527 - -1.5 811,527 - -0.4 811,533 - -0.4 811,596 + BBR_RS13550 0.20 +0.7 811,613 + BBR_RS13550 0.23 +0.7 811,640 + BBR_RS13550 0.27 +0.2 811,675 - BBR_RS13550 0.33 -0.8 811,678 + BBR_RS13550 0.33 -0.4 811,678 + BBR_RS13550 0.33 -0.5 811,720 + BBR_RS13550 0.40 -0.5 811,736 + BBR_RS13550 0.43 -0.1 811,746 + BBR_RS13550 0.44 +0.4 811,759 - BBR_RS13550 0.46 +0.7 811,787 - BBR_RS13550 0.51 -0.4 811,811 + BBR_RS13550 0.55 -0.1 811,844 + BBR_RS13550 0.60 +0.8 811,859 + BBR_RS13550 0.63 -0.0 811,859 + BBR_RS13550 0.63 +0.4 811,859 + BBR_RS13550 0.63 -0.4 811,860 - BBR_RS13550 0.63 +0.1 811,865 - BBR_RS13550 0.64 +0.6 811,867 - BBR_RS13550 0.64 +0.2 811,880 - BBR_RS13550 0.66 -1.8 811,886 - BBR_RS13550 0.67 -0.3 811,965 + BBR_RS13550 0.80 +0.4 811,975 + BBR_RS13550 0.81 -0.8 811,981 - BBR_RS13550 0.82 +1.0 811,997 + BBR_RS13550 0.85 +0.3 812,002 + BBR_RS13550 0.86 -0.0 812,016 + BBR_RS13550 0.88 +0.1 812,028 + +0.2 812,177 + BBR_RS20780 0.55 +1.2 812,185 + BBR_RS20780 0.58 +0.9 812,226 - BBR_RS20780 0.72 -0.2 812,272 + BBR_RS20780 0.88 -0.2 812,275 + BBR_RS20780 0.89 -0.2 812,281 - +0.5 812,289 + -0.4 812,294 + -0.4 812,336 - +0.2 812,359 + -0.4 812,408 + +0.7 812,491 + +0.2 812,496 + +0.1 812,524 - +0.4 812,534 - +0.3 812,534 - +0.5 812,541 + -0.8 812,546 + -0.2 812,546 + +0.0 812,546 + -0.3 812,554 - BBR_RS13555 0.10 -0.2 812,573 - BBR_RS13555 0.11 -0.2 812,611 - BBR_RS13555 0.12 -0.7 812,632 + BBR_RS13555 0.13 -1.1 812,632 + BBR_RS13555 0.13 -1.8 812,632 + BBR_RS13555 0.13 +0.2 812,632 + BBR_RS13555 0.13 -1.0 812,640 - BBR_RS13555 0.13 -0.0 812,640 - BBR_RS13555 0.13 +0.7 812,640 - BBR_RS13555 0.13 -0.7 812,640 - BBR_RS13555 0.13 +0.3 812,640 - BBR_RS13555 0.13 +1.6 812,640 - BBR_RS13555 0.13 -0.1 812,655 - BBR_RS13555 0.14 -0.1 812,667 + BBR_RS13555 0.15 -0.6 812,731 + BBR_RS13555 0.17 +0.1 812,740 - BBR_RS13555 0.17 -0.6 812,740 - BBR_RS13555 0.17 +0.2 812,742 + BBR_RS13555 0.18 +0.6 812,745 - BBR_RS13555 0.18 -0.0 812,818 - BBR_RS13555 0.21 -0.8 812,839 + BBR_RS13555 0.21 -1.4 812,865 + BBR_RS13555 0.22 -0.2 812,930 - BBR_RS13555 0.25 +1.1 812,939 - BBR_RS13555 0.25 -0.1 812,944 - BBR_RS13555 0.26 -0.5 812,991 + BBR_RS13555 0.27 -0.0 812,991 + BBR_RS13555 0.27 +0.2 812,999 - BBR_RS13555 0.28 +0.2 813,094 + BBR_RS13555 0.32 -0.6 813,094 + BBR_RS13555 0.32 -0.5 813,094 + BBR_RS13555 0.32 +1.9 813,094 + BBR_RS13555 0.32 +1.1 813,101 + BBR_RS13555 0.32 +0.9 813,106 + BBR_RS13555 0.32 +0.2 813,109 - BBR_RS13555 0.32 -0.3 813,113 + BBR_RS13555 0.32 -0.5 813,118 - BBR_RS13555 0.33 +0.3 813,135 - BBR_RS13555 0.33 -1.0 813,149 + BBR_RS13555 0.34 +0.4 813,157 - BBR_RS13555 0.34 -0.4 813,157 - BBR_RS13555 0.34 +0.7 813,157 - BBR_RS13555 0.34 -0.1 813,231 + BBR_RS13555 0.37 +0.3 813,239 - BBR_RS13555 0.37 +0.0 813,243 - BBR_RS13555 0.38 -1.4 813,246 + BBR_RS13555 0.38 +0.2 813,246 + BBR_RS13555 0.38 -1.2 813,265 - BBR_RS13555 0.38 +0.2 813,277 - BBR_RS13555 0.39 -0.1
Or see this region's nucleotide sequence