Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12520 and BBR_RS20710 are separated by 206 nucleotides BBR_RS20710 and BBR_RS12525 are separated by 69 nucleotides BBR_RS12525 and BBR_RS12530 overlap by 1 nucleotides
BBR_RS12520: BBR_RS12520 - glycosyl hydrolase family 53 Endogalactanase galactan metabolism, at 562,557 to 565,250
_RS12520
BBR_RS20710: BBR_RS20710 - hypothetical protein, at 565,457 to 565,854
_RS20710
BBR_RS12525: BBR_RS12525 - aldo/keto reductase, at 565,924 to 566,793
_RS12525
BBR_RS12530: BBR_RS12530 - HNH endonuclease, at 566,793 to 567,551
_RS12530
Position (kb) 565
566 Strain fitness (log2 ratio) -2
-1
0
1 at 564.465 kb on + strand, within BBR_RS12520 at 564.467 kb on - strand, within BBR_RS12520 at 564.473 kb on + strand, within BBR_RS12520 at 564.530 kb on + strand, within BBR_RS12520 at 564.530 kb on + strand, within BBR_RS12520 at 564.553 kb on - strand, within BBR_RS12520 at 564.553 kb on - strand, within BBR_RS12520 at 564.599 kb on + strand, within BBR_RS12520 at 564.607 kb on - strand, within BBR_RS12520 at 564.607 kb on - strand, within BBR_RS12520 at 564.631 kb on + strand, within BBR_RS12520 at 564.647 kb on - strand, within BBR_RS12520 at 564.710 kb on + strand, within BBR_RS12520 at 564.710 kb on + strand, within BBR_RS12520 at 564.710 kb on + strand, within BBR_RS12520 at 564.710 kb on + strand, within BBR_RS12520 at 564.717 kb on - strand, within BBR_RS12520 at 564.718 kb on - strand, within BBR_RS12520 at 564.718 kb on - strand, within BBR_RS12520 at 564.718 kb on - strand, within BBR_RS12520 at 564.748 kb on - strand, within BBR_RS12520 at 564.775 kb on + strand, within BBR_RS12520 at 564.775 kb on + strand, within BBR_RS12520 at 564.775 kb on + strand, within BBR_RS12520 at 564.775 kb on + strand, within BBR_RS12520 at 564.783 kb on - strand, within BBR_RS12520 at 564.783 kb on - strand, within BBR_RS12520 at 564.783 kb on - strand, within BBR_RS12520 at 564.783 kb on - strand, within BBR_RS12520 at 564.783 kb on - strand, within BBR_RS12520 at 564.791 kb on + strand, within BBR_RS12520 at 564.795 kb on - strand, within BBR_RS12520 at 564.805 kb on + strand, within BBR_RS12520 at 564.835 kb on + strand, within BBR_RS12520 at 564.857 kb on + strand, within BBR_RS12520 at 564.875 kb on + strand, within BBR_RS12520 at 564.885 kb on + strand, within BBR_RS12520 at 564.885 kb on + strand, within BBR_RS12520 at 564.885 kb on + strand, within BBR_RS12520 at 564.893 kb on - strand, within BBR_RS12520 at 564.915 kb on - strand, within BBR_RS12520 at 564.917 kb on + strand, within BBR_RS12520 at 564.945 kb on + strand, within BBR_RS12520 at 564.945 kb on + strand, within BBR_RS12520 at 564.970 kb on - strand, within BBR_RS12520 at 564.987 kb on - strand at 564.996 kb on + strand at 565.003 kb on + strand at 565.003 kb on + strand at 565.003 kb on - strand at 565.012 kb on - strand at 565.051 kb on + strand at 565.072 kb on - strand at 565.095 kb on - strand at 565.112 kb on + strand at 565.115 kb on - strand at 565.115 kb on - strand at 565.136 kb on + strand at 565.136 kb on + strand at 565.136 kb on + strand at 565.136 kb on + strand at 565.139 kb on - strand at 565.151 kb on + strand at 565.154 kb on - strand at 565.174 kb on + strand at 565.174 kb on + strand at 565.176 kb on + strand at 565.183 kb on + strand at 565.183 kb on + strand at 565.230 kb on - strand at 565.242 kb on - strand at 565.278 kb on + strand at 565.284 kb on - strand at 565.286 kb on - strand at 565.322 kb on - strand at 565.322 kb on - strand at 565.330 kb on + strand at 565.380 kb on - strand at 565.410 kb on + strand at 565.410 kb on + strand at 565.448 kb on - strand at 565.554 kb on - strand, within BBR_RS20710 at 565.554 kb on - strand, within BBR_RS20710 at 565.627 kb on - strand, within BBR_RS20710 at 565.632 kb on - strand, within BBR_RS20710 at 565.710 kb on - strand, within BBR_RS20710 at 565.715 kb on - strand, within BBR_RS20710 at 565.739 kb on + strand, within BBR_RS20710 at 565.745 kb on + strand, within BBR_RS20710 at 565.797 kb on - strand, within BBR_RS20710 at 565.799 kb on + strand, within BBR_RS20710 at 565.799 kb on + strand, within BBR_RS20710 at 565.813 kb on + strand, within BBR_RS20710 at 565.844 kb on + strand at 565.844 kb on + strand at 565.858 kb on + strand at 565.860 kb on + strand at 565.860 kb on + strand at 565.860 kb on + strand at 565.865 kb on + strand at 565.865 kb on + strand at 565.865 kb on + strand at 565.865 kb on + strand at 565.865 kb on + strand at 565.870 kb on + strand at 565.873 kb on - strand at 565.877 kb on + strand at 565.878 kb on - strand at 565.885 kb on - strand at 565.885 kb on - strand at 565.902 kb on + strand at 565.902 kb on + strand at 565.902 kb on + strand at 565.902 kb on + strand at 565.908 kb on - strand at 565.910 kb on - strand at 565.920 kb on + strand at 565.936 kb on - strand at 565.938 kb on + strand at 565.938 kb on + strand at 565.946 kb on - strand at 565.954 kb on + strand at 566.000 kb on + strand at 566.008 kb on - strand at 566.027 kb on + strand, within BBR_RS12525 at 566.028 kb on + strand, within BBR_RS12525 at 566.029 kb on + strand, within BBR_RS12525 at 566.029 kb on + strand, within BBR_RS12525 at 566.037 kb on - strand, within BBR_RS12525 at 566.041 kb on + strand, within BBR_RS12525 at 566.049 kb on - strand, within BBR_RS12525 at 566.095 kb on - strand, within BBR_RS12525 at 566.095 kb on - strand, within BBR_RS12525 at 566.118 kb on - strand, within BBR_RS12525 at 566.118 kb on - strand, within BBR_RS12525 at 566.118 kb on - strand, within BBR_RS12525 at 566.118 kb on - strand, within BBR_RS12525 at 566.118 kb on - strand, within BBR_RS12525 at 566.137 kb on + strand, within BBR_RS12525 at 566.140 kb on - strand, within BBR_RS12525 at 566.145 kb on - strand, within BBR_RS12525 at 566.153 kb on - strand, within BBR_RS12525 at 566.157 kb on + strand, within BBR_RS12525 at 566.157 kb on + strand, within BBR_RS12525 at 566.157 kb on + strand, within BBR_RS12525 at 566.177 kb on + strand, within BBR_RS12525 at 566.177 kb on + strand, within BBR_RS12525 at 566.177 kb on + strand, within BBR_RS12525 at 566.177 kb on + strand, within BBR_RS12525 at 566.185 kb on - strand, within BBR_RS12525 at 566.185 kb on - strand, within BBR_RS12525 at 566.185 kb on - strand, within BBR_RS12525 at 566.195 kb on - strand, within BBR_RS12525 at 566.195 kb on - strand, within BBR_RS12525 at 566.200 kb on - strand, within BBR_RS12525 at 566.200 kb on - strand, within BBR_RS12525 at 566.202 kb on + strand, within BBR_RS12525 at 566.203 kb on + strand, within BBR_RS12525 at 566.203 kb on + strand, within BBR_RS12525 at 566.210 kb on - strand, within BBR_RS12525 at 566.211 kb on - strand, within BBR_RS12525 at 566.211 kb on - strand, within BBR_RS12525 at 566.211 kb on - strand, within BBR_RS12525 at 566.229 kb on + strand, within BBR_RS12525 at 566.237 kb on + strand, within BBR_RS12525 at 566.237 kb on + strand, within BBR_RS12525 at 566.237 kb on + strand, within BBR_RS12525 at 566.245 kb on - strand, within BBR_RS12525 at 566.268 kb on + strand, within BBR_RS12525 at 566.271 kb on + strand, within BBR_RS12525 at 566.285 kb on + strand, within BBR_RS12525 at 566.285 kb on + strand, within BBR_RS12525 at 566.293 kb on - strand, within BBR_RS12525 at 566.293 kb on - strand, within BBR_RS12525 at 566.301 kb on - strand, within BBR_RS12525 at 566.309 kb on + strand, within BBR_RS12525 at 566.312 kb on - strand, within BBR_RS12525 at 566.318 kb on + strand, within BBR_RS12525 at 566.318 kb on + strand, within BBR_RS12525 at 566.318 kb on + strand, within BBR_RS12525 at 566.345 kb on + strand, within BBR_RS12525 at 566.348 kb on - strand, within BBR_RS12525 at 566.348 kb on - strand, within BBR_RS12525 at 566.350 kb on + strand, within BBR_RS12525 at 566.350 kb on + strand, within BBR_RS12525 at 566.358 kb on - strand, within BBR_RS12525 at 566.358 kb on - strand, within BBR_RS12525 at 566.358 kb on - strand, within BBR_RS12525 at 566.358 kb on - strand, within BBR_RS12525 at 566.358 kb on - strand, within BBR_RS12525 at 566.358 kb on - strand, within BBR_RS12525 at 566.358 kb on - strand, within BBR_RS12525 at 566.358 kb on - strand, within BBR_RS12525 at 566.358 kb on - strand, within BBR_RS12525 at 566.371 kb on - strand, within BBR_RS12525 at 566.371 kb on - strand, within BBR_RS12525 at 566.402 kb on - strand, within BBR_RS12525 at 566.415 kb on + strand, within BBR_RS12525 at 566.423 kb on - strand, within BBR_RS12525 at 566.428 kb on - strand, within BBR_RS12525 at 566.442 kb on + strand, within BBR_RS12525 at 566.442 kb on + strand, within BBR_RS12525 at 566.456 kb on + strand, within BBR_RS12525 at 566.459 kb on + strand, within BBR_RS12525 at 566.467 kb on - strand, within BBR_RS12525 at 566.494 kb on + strand, within BBR_RS12525 at 566.494 kb on + strand, within BBR_RS12525 at 566.494 kb on + strand, within BBR_RS12525 at 566.494 kb on + strand, within BBR_RS12525 at 566.494 kb on + strand, within BBR_RS12525 at 566.494 kb on + strand, within BBR_RS12525 at 566.502 kb on - strand, within BBR_RS12525 at 566.502 kb on - strand, within BBR_RS12525 at 566.507 kb on + strand, within BBR_RS12525 at 566.507 kb on + strand, within BBR_RS12525 at 566.522 kb on - strand, within BBR_RS12525 at 566.545 kb on - strand, within BBR_RS12525 at 566.566 kb on + strand, within BBR_RS12525 at 566.566 kb on + strand, within BBR_RS12525 at 566.574 kb on - strand, within BBR_RS12525 at 566.574 kb on - strand, within BBR_RS12525 at 566.597 kb on + strand, within BBR_RS12525 at 566.597 kb on + strand, within BBR_RS12525 at 566.647 kb on + strand, within BBR_RS12525 at 566.684 kb on + strand, within BBR_RS12525 at 566.692 kb on - strand, within BBR_RS12525 at 566.692 kb on - strand, within BBR_RS12525 at 566.694 kb on + strand, within BBR_RS12525 at 566.699 kb on + strand, within BBR_RS12525 at 566.721 kb on + strand at 566.745 kb on - strand at 566.745 kb on - strand at 566.746 kb on + strand at 566.762 kb on + strand at 566.769 kb on - strand at 566.778 kb on - strand at 566.782 kb on + strand at 566.784 kb on - strand at 566.784 kb on - strand at 566.787 kb on + strand at 566.796 kb on + strand at 566.804 kb on + strand at 566.804 kb on + strand at 566.804 kb on - strand at 566.851 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 564,465 + BBR_RS12520 0.71 +0.4 564,467 - BBR_RS12520 0.71 +0.5 564,473 + BBR_RS12520 0.71 -1.1 564,530 + BBR_RS12520 0.73 -0.0 564,530 + BBR_RS12520 0.73 +0.1 564,553 - BBR_RS12520 0.74 +0.3 564,553 - BBR_RS12520 0.74 +0.3 564,599 + BBR_RS12520 0.76 +0.4 564,607 - BBR_RS12520 0.76 -0.6 564,607 - BBR_RS12520 0.76 +0.0 564,631 + BBR_RS12520 0.77 +0.4 564,647 - BBR_RS12520 0.78 -0.2 564,710 + BBR_RS12520 0.80 +0.0 564,710 + BBR_RS12520 0.80 -0.2 564,710 + BBR_RS12520 0.80 -0.0 564,710 + BBR_RS12520 0.80 -0.1 564,717 - BBR_RS12520 0.80 +0.2 564,718 - BBR_RS12520 0.80 -0.2 564,718 - BBR_RS12520 0.80 +0.1 564,718 - BBR_RS12520 0.80 +0.3 564,748 - BBR_RS12520 0.81 +0.3 564,775 + BBR_RS12520 0.82 +0.6 564,775 + BBR_RS12520 0.82 +1.4 564,775 + BBR_RS12520 0.82 +0.8 564,775 + BBR_RS12520 0.82 +0.6 564,783 - BBR_RS12520 0.83 +0.2 564,783 - BBR_RS12520 0.83 +0.0 564,783 - BBR_RS12520 0.83 +1.2 564,783 - BBR_RS12520 0.83 -0.2 564,783 - BBR_RS12520 0.83 -0.0 564,791 + BBR_RS12520 0.83 +0.1 564,795 - BBR_RS12520 0.83 -0.0 564,805 + BBR_RS12520 0.83 +0.5 564,835 + BBR_RS12520 0.85 +0.1 564,857 + BBR_RS12520 0.85 +0.3 564,875 + BBR_RS12520 0.86 +0.7 564,885 + BBR_RS12520 0.86 +1.0 564,885 + BBR_RS12520 0.86 -1.4 564,885 + BBR_RS12520 0.86 +0.3 564,893 - BBR_RS12520 0.87 +0.3 564,915 - BBR_RS12520 0.88 +0.4 564,917 + BBR_RS12520 0.88 +0.5 564,945 + BBR_RS12520 0.89 -0.1 564,945 + BBR_RS12520 0.89 +0.2 564,970 - BBR_RS12520 0.90 +0.1 564,987 - +0.0 564,996 + +0.6 565,003 + +0.6 565,003 + +0.5 565,003 - +1.2 565,012 - +0.1 565,051 + +1.0 565,072 - -0.2 565,095 - +0.9 565,112 + -0.1 565,115 - -0.3 565,115 - -2.5 565,136 + +0.1 565,136 + +0.5 565,136 + +1.1 565,136 + +0.7 565,139 - +0.3 565,151 + -0.3 565,154 - -0.1 565,174 + +0.0 565,174 + -0.0 565,176 + -1.1 565,183 + +0.3 565,183 + -0.6 565,230 - -0.2 565,242 - +1.0 565,278 + -0.3 565,284 - +0.5 565,286 - -1.3 565,322 - -0.4 565,322 - -0.1 565,330 + +0.1 565,380 - -0.2 565,410 + +0.8 565,410 + -0.5 565,448 - -0.6 565,554 - BBR_RS20710 0.24 +0.1 565,554 - BBR_RS20710 0.24 +0.5 565,627 - BBR_RS20710 0.43 -0.1 565,632 - BBR_RS20710 0.44 +1.1 565,710 - BBR_RS20710 0.64 +0.8 565,715 - BBR_RS20710 0.65 +0.7 565,739 + BBR_RS20710 0.71 -0.1 565,745 + BBR_RS20710 0.72 +0.1 565,797 - BBR_RS20710 0.85 +0.3 565,799 + BBR_RS20710 0.86 +0.3 565,799 + BBR_RS20710 0.86 +0.3 565,813 + BBR_RS20710 0.89 -0.4 565,844 + -0.2 565,844 + -0.1 565,858 + +0.3 565,860 + -0.1 565,860 + +0.3 565,860 + +0.8 565,865 + -0.7 565,865 + +1.2 565,865 + -0.3 565,865 + -0.1 565,865 + -0.1 565,870 + -0.7 565,873 - +0.0 565,877 + +0.9 565,878 - +0.0 565,885 - +0.2 565,885 - -0.3 565,902 + +0.4 565,902 + +0.6 565,902 + -0.0 565,902 + -0.2 565,908 - -0.3 565,910 - +0.6 565,920 + -0.1 565,936 - +0.4 565,938 + +1.1 565,938 + +0.0 565,946 - +0.1 565,954 + -0.9 566,000 + -0.1 566,008 - -0.4 566,027 + BBR_RS12525 0.12 -0.0 566,028 + BBR_RS12525 0.12 -0.3 566,029 + BBR_RS12525 0.12 -0.0 566,029 + BBR_RS12525 0.12 -0.2 566,037 - BBR_RS12525 0.13 -0.4 566,041 + BBR_RS12525 0.13 -0.2 566,049 - BBR_RS12525 0.14 +0.4 566,095 - BBR_RS12525 0.20 +1.0 566,095 - BBR_RS12525 0.20 +0.1 566,118 - BBR_RS12525 0.22 -0.3 566,118 - BBR_RS12525 0.22 -0.8 566,118 - BBR_RS12525 0.22 +0.4 566,118 - BBR_RS12525 0.22 +0.2 566,118 - BBR_RS12525 0.22 +0.1 566,137 + BBR_RS12525 0.24 -0.9 566,140 - BBR_RS12525 0.25 +0.3 566,145 - BBR_RS12525 0.25 -0.0 566,153 - BBR_RS12525 0.26 -0.4 566,157 + BBR_RS12525 0.27 +0.9 566,157 + BBR_RS12525 0.27 +0.2 566,157 + BBR_RS12525 0.27 +0.3 566,177 + BBR_RS12525 0.29 +0.0 566,177 + BBR_RS12525 0.29 -0.2 566,177 + BBR_RS12525 0.29 +0.4 566,177 + BBR_RS12525 0.29 -0.7 566,185 - BBR_RS12525 0.30 +0.2 566,185 - BBR_RS12525 0.30 +0.9 566,185 - BBR_RS12525 0.30 +0.9 566,195 - BBR_RS12525 0.31 +0.1 566,195 - BBR_RS12525 0.31 +0.4 566,200 - BBR_RS12525 0.32 -0.4 566,200 - BBR_RS12525 0.32 -0.1 566,202 + BBR_RS12525 0.32 +0.8 566,203 + BBR_RS12525 0.32 +0.8 566,203 + BBR_RS12525 0.32 -0.0 566,210 - BBR_RS12525 0.33 +0.2 566,211 - BBR_RS12525 0.33 +0.2 566,211 - BBR_RS12525 0.33 +0.2 566,211 - BBR_RS12525 0.33 +0.2 566,229 + BBR_RS12525 0.35 -0.5 566,237 + BBR_RS12525 0.36 -0.1 566,237 + BBR_RS12525 0.36 +0.3 566,237 + BBR_RS12525 0.36 -0.2 566,245 - BBR_RS12525 0.37 +0.4 566,268 + BBR_RS12525 0.40 +0.9 566,271 + BBR_RS12525 0.40 -0.6 566,285 + BBR_RS12525 0.41 +0.4 566,285 + BBR_RS12525 0.41 +0.2 566,293 - BBR_RS12525 0.42 -0.4 566,293 - BBR_RS12525 0.42 +0.6 566,301 - BBR_RS12525 0.43 +0.2 566,309 + BBR_RS12525 0.44 +0.8 566,312 - BBR_RS12525 0.45 +0.7 566,318 + BBR_RS12525 0.45 +1.1 566,318 + BBR_RS12525 0.45 +0.2 566,318 + BBR_RS12525 0.45 +0.2 566,345 + BBR_RS12525 0.48 +0.1 566,348 - BBR_RS12525 0.49 -0.4 566,348 - BBR_RS12525 0.49 +0.7 566,350 + BBR_RS12525 0.49 +0.2 566,350 + BBR_RS12525 0.49 +0.7 566,358 - BBR_RS12525 0.50 +0.7 566,358 - BBR_RS12525 0.50 +0.0 566,358 - BBR_RS12525 0.50 +0.0 566,358 - BBR_RS12525 0.50 +0.4 566,358 - BBR_RS12525 0.50 -0.2 566,358 - BBR_RS12525 0.50 -0.9 566,358 - BBR_RS12525 0.50 -0.3 566,358 - BBR_RS12525 0.50 -0.9 566,358 - BBR_RS12525 0.50 -0.0 566,371 - BBR_RS12525 0.51 -0.1 566,371 - BBR_RS12525 0.51 +0.4 566,402 - BBR_RS12525 0.55 +0.1 566,415 + BBR_RS12525 0.56 +0.4 566,423 - BBR_RS12525 0.57 +0.6 566,428 - BBR_RS12525 0.58 -0.6 566,442 + BBR_RS12525 0.60 -0.1 566,442 + BBR_RS12525 0.60 +0.7 566,456 + BBR_RS12525 0.61 +0.1 566,459 + BBR_RS12525 0.61 +0.5 566,467 - BBR_RS12525 0.62 -0.1 566,494 + BBR_RS12525 0.66 +1.5 566,494 + BBR_RS12525 0.66 +0.6 566,494 + BBR_RS12525 0.66 -0.1 566,494 + BBR_RS12525 0.66 +0.0 566,494 + BBR_RS12525 0.66 +0.2 566,494 + BBR_RS12525 0.66 +0.4 566,502 - BBR_RS12525 0.66 -0.4 566,502 - BBR_RS12525 0.66 -0.1 566,507 + BBR_RS12525 0.67 -0.0 566,507 + BBR_RS12525 0.67 -0.0 566,522 - BBR_RS12525 0.69 +0.6 566,545 - BBR_RS12525 0.71 -0.3 566,566 + BBR_RS12525 0.74 -0.7 566,566 + BBR_RS12525 0.74 +0.3 566,574 - BBR_RS12525 0.75 -1.4 566,574 - BBR_RS12525 0.75 +0.6 566,597 + BBR_RS12525 0.77 +0.3 566,597 + BBR_RS12525 0.77 +0.1 566,647 + BBR_RS12525 0.83 +0.0 566,684 + BBR_RS12525 0.87 -0.1 566,692 - BBR_RS12525 0.88 -0.0 566,692 - BBR_RS12525 0.88 -0.4 566,694 + BBR_RS12525 0.89 -0.6 566,699 + BBR_RS12525 0.89 -0.0 566,721 + +1.2 566,745 - +0.0 566,745 - -0.0 566,746 + +0.5 566,762 + +0.6 566,769 - +0.4 566,778 - +0.1 566,782 + -0.4 566,784 - +0.3 566,784 - +0.3 566,787 + +0.2 566,796 + -0.3 566,804 + -0.4 566,804 + +0.4 566,804 - +0.6 566,851 + +0.3
Or see this region's nucleotide sequence