Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS18645

Experiment: Recovery broth with Isovaleric 75 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS18640 and BBR_RS18645 overlap by 4 nucleotidesBBR_RS18645 and BBR_RS18650 are separated by 125 nucleotides BBR_RS18640: BBR_RS18640 - PspC domain-containing protein, at 1,968,613 to 1,969,899 _RS18640 BBR_RS18645: BBR_RS18645 - DNA-binding response regulator, at 1,969,896 to 1,970,579 _RS18645 BBR_RS18650: BBR_RS18650 - UDP-glucose 4-epimerase GalE, at 1,970,705 to 1,971,727 _RS18650 Position (kb) 1969 1970 1971Strain fitness (log2 ratio) -3 -2 -1 0 1at 1968.898 kb on - strand, within BBR_RS18640at 1968.908 kb on - strand, within BBR_RS18640at 1968.908 kb on - strand, within BBR_RS18640at 1968.933 kb on + strand, within BBR_RS18640at 1968.987 kb on - strand, within BBR_RS18640at 1969.002 kb on - strand, within BBR_RS18640at 1969.069 kb on - strand, within BBR_RS18640at 1969.079 kb on - strand, within BBR_RS18640at 1969.081 kb on + strand, within BBR_RS18640at 1969.120 kb on - strand, within BBR_RS18640at 1969.120 kb on - strand, within BBR_RS18640at 1969.172 kb on - strand, within BBR_RS18640at 1969.174 kb on + strand, within BBR_RS18640at 1969.208 kb on - strand, within BBR_RS18640at 1969.313 kb on + strand, within BBR_RS18640at 1969.428 kb on - strand, within BBR_RS18640at 1969.428 kb on - strand, within BBR_RS18640at 1969.428 kb on - strand, within BBR_RS18640at 1969.510 kb on - strand, within BBR_RS18640at 1969.530 kb on + strand, within BBR_RS18640at 1969.640 kb on - strand, within BBR_RS18640at 1969.647 kb on - strand, within BBR_RS18640at 1969.665 kb on - strand, within BBR_RS18640at 1969.667 kb on + strand, within BBR_RS18640at 1969.681 kb on - strand, within BBR_RS18640at 1969.861 kb on - strandat 1969.914 kb on + strandat 1969.926 kb on - strandat 1969.960 kb on - strandat 1969.977 kb on - strand, within BBR_RS18645at 1969.987 kb on + strand, within BBR_RS18645at 1969.987 kb on - strand, within BBR_RS18645at 1970.045 kb on + strand, within BBR_RS18645at 1970.065 kb on + strand, within BBR_RS18645at 1970.084 kb on - strand, within BBR_RS18645at 1970.094 kb on + strand, within BBR_RS18645at 1970.120 kb on - strand, within BBR_RS18645at 1970.120 kb on - strand, within BBR_RS18645at 1970.145 kb on + strand, within BBR_RS18645at 1970.145 kb on + strand, within BBR_RS18645at 1970.148 kb on + strand, within BBR_RS18645at 1970.148 kb on - strand, within BBR_RS18645at 1970.158 kb on - strand, within BBR_RS18645at 1970.158 kb on - strand, within BBR_RS18645at 1970.166 kb on + strand, within BBR_RS18645at 1970.174 kb on - strand, within BBR_RS18645at 1970.186 kb on - strand, within BBR_RS18645at 1970.191 kb on - strand, within BBR_RS18645at 1970.211 kb on + strand, within BBR_RS18645at 1970.211 kb on + strand, within BBR_RS18645at 1970.211 kb on + strand, within BBR_RS18645at 1970.211 kb on + strand, within BBR_RS18645at 1970.211 kb on + strand, within BBR_RS18645at 1970.211 kb on + strand, within BBR_RS18645at 1970.211 kb on + strand, within BBR_RS18645at 1970.219 kb on - strand, within BBR_RS18645at 1970.282 kb on - strand, within BBR_RS18645at 1970.282 kb on - strand, within BBR_RS18645at 1970.296 kb on + strand, within BBR_RS18645at 1970.296 kb on + strand, within BBR_RS18645at 1970.390 kb on - strand, within BBR_RS18645at 1970.443 kb on + strand, within BBR_RS18645at 1970.443 kb on + strand, within BBR_RS18645at 1970.451 kb on - strand, within BBR_RS18645at 1970.451 kb on - strand, within BBR_RS18645at 1970.487 kb on + strand, within BBR_RS18645at 1970.495 kb on - strand, within BBR_RS18645at 1970.511 kb on - strandat 1970.520 kb on - strandat 1970.542 kb on + strandat 1970.557 kb on + strandat 1970.557 kb on + strandat 1970.581 kb on + strandat 1970.589 kb on - strandat 1970.608 kb on - strandat 1970.632 kb on + strandat 1970.632 kb on + strandat 1970.640 kb on - strandat 1970.663 kb on + strandat 1970.724 kb on + strandat 1970.732 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM
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1,968,898 - BBR_RS18640 0.22 +0.7
1,968,908 - BBR_RS18640 0.23 -0.3
1,968,908 - BBR_RS18640 0.23 +1.1
1,968,933 + BBR_RS18640 0.25 -1.6
1,968,987 - BBR_RS18640 0.29 -1.1
1,969,002 - BBR_RS18640 0.30 +0.0
1,969,069 - BBR_RS18640 0.35 -1.1
1,969,079 - BBR_RS18640 0.36 +0.2
1,969,081 + BBR_RS18640 0.36 -0.2
1,969,120 - BBR_RS18640 0.39 +0.9
1,969,120 - BBR_RS18640 0.39 -0.6
1,969,172 - BBR_RS18640 0.43 +0.6
1,969,174 + BBR_RS18640 0.44 -1.6
1,969,208 - BBR_RS18640 0.46 -0.3
1,969,313 + BBR_RS18640 0.54 -3.3
1,969,428 - BBR_RS18640 0.63 +0.1
1,969,428 - BBR_RS18640 0.63 -1.7
1,969,428 - BBR_RS18640 0.63 -0.3
1,969,510 - BBR_RS18640 0.70 +1.0
1,969,530 + BBR_RS18640 0.71 -1.2
1,969,640 - BBR_RS18640 0.80 +0.1
1,969,647 - BBR_RS18640 0.80 -0.2
1,969,665 - BBR_RS18640 0.82 -0.1
1,969,667 + BBR_RS18640 0.82 -1.9
1,969,681 - BBR_RS18640 0.83 -1.2
1,969,861 - +0.2
1,969,914 + +0.4
1,969,926 - -0.4
1,969,960 - +0.1
1,969,977 - BBR_RS18645 0.12 +0.5
1,969,987 + BBR_RS18645 0.13 +0.5
1,969,987 - BBR_RS18645 0.13 +0.4
1,970,045 + BBR_RS18645 0.22 -0.3
1,970,065 + BBR_RS18645 0.25 +0.9
1,970,084 - BBR_RS18645 0.27 +0.7
1,970,094 + BBR_RS18645 0.29 +1.5
1,970,120 - BBR_RS18645 0.33 +0.5
1,970,120 - BBR_RS18645 0.33 +0.2
1,970,145 + BBR_RS18645 0.36 +0.2
1,970,145 + BBR_RS18645 0.36 +0.7
1,970,148 + BBR_RS18645 0.37 +0.4
1,970,148 - BBR_RS18645 0.37 +0.6
1,970,158 - BBR_RS18645 0.38 +0.1
1,970,158 - BBR_RS18645 0.38 +1.4
1,970,166 + BBR_RS18645 0.39 +0.5
1,970,174 - BBR_RS18645 0.41 +1.0
1,970,186 - BBR_RS18645 0.42 +0.8
1,970,191 - BBR_RS18645 0.43 +0.7
1,970,211 + BBR_RS18645 0.46 +0.5
1,970,211 + BBR_RS18645 0.46 -0.5
1,970,211 + BBR_RS18645 0.46 +0.2
1,970,211 + BBR_RS18645 0.46 +0.4
1,970,211 + BBR_RS18645 0.46 +0.0
1,970,211 + BBR_RS18645 0.46 +0.8
1,970,211 + BBR_RS18645 0.46 +0.5
1,970,219 - BBR_RS18645 0.47 +0.2
1,970,282 - BBR_RS18645 0.56 +1.2
1,970,282 - BBR_RS18645 0.56 +0.6
1,970,296 + BBR_RS18645 0.58 +1.0
1,970,296 + BBR_RS18645 0.58 +0.4
1,970,390 - BBR_RS18645 0.72 -0.2
1,970,443 + BBR_RS18645 0.80 +1.4
1,970,443 + BBR_RS18645 0.80 -0.0
1,970,451 - BBR_RS18645 0.81 +0.6
1,970,451 - BBR_RS18645 0.81 +0.4
1,970,487 + BBR_RS18645 0.86 +0.2
1,970,495 - BBR_RS18645 0.88 +0.5
1,970,511 - +0.4
1,970,520 - +0.2
1,970,542 + -0.2
1,970,557 + +0.3
1,970,557 + +0.6
1,970,581 + +0.5
1,970,589 - +0.2
1,970,608 - -0.5
1,970,632 + +0.3
1,970,632 + -0.6
1,970,640 - -0.1
1,970,663 + +0.3
1,970,724 + +0.2
1,970,732 - -0.2

Or see this region's nucleotide sequence