Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18640 and BBR_RS18645 overlap by 4 nucleotides BBR_RS18645 and BBR_RS18650 are separated by 125 nucleotides
BBR_RS18640: BBR_RS18640 - PspC domain-containing protein, at 1,968,613 to 1,969,899
_RS18640
BBR_RS18645: BBR_RS18645 - DNA-binding response regulator, at 1,969,896 to 1,970,579
_RS18645
BBR_RS18650: BBR_RS18650 - UDP-glucose 4-epimerase GalE, at 1,970,705 to 1,971,727
_RS18650
Position (kb)
1969
1970
1971 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1968.898 kb on - strand, within BBR_RS18640 at 1968.908 kb on - strand, within BBR_RS18640 at 1968.908 kb on - strand, within BBR_RS18640 at 1968.933 kb on + strand, within BBR_RS18640 at 1968.987 kb on - strand, within BBR_RS18640 at 1969.002 kb on - strand, within BBR_RS18640 at 1969.069 kb on - strand, within BBR_RS18640 at 1969.079 kb on - strand, within BBR_RS18640 at 1969.081 kb on + strand, within BBR_RS18640 at 1969.120 kb on - strand, within BBR_RS18640 at 1969.120 kb on - strand, within BBR_RS18640 at 1969.172 kb on - strand, within BBR_RS18640 at 1969.174 kb on + strand, within BBR_RS18640 at 1969.208 kb on - strand, within BBR_RS18640 at 1969.313 kb on + strand, within BBR_RS18640 at 1969.428 kb on - strand, within BBR_RS18640 at 1969.428 kb on - strand, within BBR_RS18640 at 1969.428 kb on - strand, within BBR_RS18640 at 1969.510 kb on - strand, within BBR_RS18640 at 1969.530 kb on + strand, within BBR_RS18640 at 1969.640 kb on - strand, within BBR_RS18640 at 1969.647 kb on - strand, within BBR_RS18640 at 1969.665 kb on - strand, within BBR_RS18640 at 1969.667 kb on + strand, within BBR_RS18640 at 1969.681 kb on - strand, within BBR_RS18640 at 1969.861 kb on - strand at 1969.914 kb on + strand at 1969.926 kb on - strand at 1969.960 kb on - strand at 1969.977 kb on - strand, within BBR_RS18645 at 1969.987 kb on + strand, within BBR_RS18645 at 1969.987 kb on - strand, within BBR_RS18645 at 1970.045 kb on + strand, within BBR_RS18645 at 1970.065 kb on + strand, within BBR_RS18645 at 1970.084 kb on - strand, within BBR_RS18645 at 1970.094 kb on + strand, within BBR_RS18645 at 1970.120 kb on - strand, within BBR_RS18645 at 1970.120 kb on - strand, within BBR_RS18645 at 1970.145 kb on + strand, within BBR_RS18645 at 1970.145 kb on + strand, within BBR_RS18645 at 1970.148 kb on + strand, within BBR_RS18645 at 1970.148 kb on - strand, within BBR_RS18645 at 1970.158 kb on - strand, within BBR_RS18645 at 1970.158 kb on - strand, within BBR_RS18645 at 1970.166 kb on + strand, within BBR_RS18645 at 1970.174 kb on - strand, within BBR_RS18645 at 1970.186 kb on - strand, within BBR_RS18645 at 1970.191 kb on - strand, within BBR_RS18645 at 1970.211 kb on + strand, within BBR_RS18645 at 1970.211 kb on + strand, within BBR_RS18645 at 1970.211 kb on + strand, within BBR_RS18645 at 1970.211 kb on + strand, within BBR_RS18645 at 1970.211 kb on + strand, within BBR_RS18645 at 1970.211 kb on + strand, within BBR_RS18645 at 1970.211 kb on + strand, within BBR_RS18645 at 1970.219 kb on - strand, within BBR_RS18645 at 1970.282 kb on - strand, within BBR_RS18645 at 1970.282 kb on - strand, within BBR_RS18645 at 1970.296 kb on + strand, within BBR_RS18645 at 1970.296 kb on + strand, within BBR_RS18645 at 1970.390 kb on - strand, within BBR_RS18645 at 1970.443 kb on + strand, within BBR_RS18645 at 1970.443 kb on + strand, within BBR_RS18645 at 1970.451 kb on - strand, within BBR_RS18645 at 1970.451 kb on - strand, within BBR_RS18645 at 1970.487 kb on + strand, within BBR_RS18645 at 1970.495 kb on - strand, within BBR_RS18645 at 1970.511 kb on - strand at 1970.520 kb on - strand at 1970.542 kb on + strand at 1970.557 kb on + strand at 1970.557 kb on + strand at 1970.581 kb on + strand at 1970.589 kb on - strand at 1970.608 kb on - strand at 1970.632 kb on + strand at 1970.632 kb on + strand at 1970.640 kb on - strand at 1970.663 kb on + strand at 1970.724 kb on + strand at 1970.732 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,968,898 - BBR_RS18640 0.22 +0.7 1,968,908 - BBR_RS18640 0.23 -0.3 1,968,908 - BBR_RS18640 0.23 +1.1 1,968,933 + BBR_RS18640 0.25 -1.6 1,968,987 - BBR_RS18640 0.29 -1.1 1,969,002 - BBR_RS18640 0.30 +0.0 1,969,069 - BBR_RS18640 0.35 -1.1 1,969,079 - BBR_RS18640 0.36 +0.2 1,969,081 + BBR_RS18640 0.36 -0.2 1,969,120 - BBR_RS18640 0.39 +0.9 1,969,120 - BBR_RS18640 0.39 -0.6 1,969,172 - BBR_RS18640 0.43 +0.6 1,969,174 + BBR_RS18640 0.44 -1.6 1,969,208 - BBR_RS18640 0.46 -0.3 1,969,313 + BBR_RS18640 0.54 -3.3 1,969,428 - BBR_RS18640 0.63 +0.1 1,969,428 - BBR_RS18640 0.63 -1.7 1,969,428 - BBR_RS18640 0.63 -0.3 1,969,510 - BBR_RS18640 0.70 +1.0 1,969,530 + BBR_RS18640 0.71 -1.2 1,969,640 - BBR_RS18640 0.80 +0.1 1,969,647 - BBR_RS18640 0.80 -0.2 1,969,665 - BBR_RS18640 0.82 -0.1 1,969,667 + BBR_RS18640 0.82 -1.9 1,969,681 - BBR_RS18640 0.83 -1.2 1,969,861 - +0.2 1,969,914 + +0.4 1,969,926 - -0.4 1,969,960 - +0.1 1,969,977 - BBR_RS18645 0.12 +0.5 1,969,987 + BBR_RS18645 0.13 +0.5 1,969,987 - BBR_RS18645 0.13 +0.4 1,970,045 + BBR_RS18645 0.22 -0.3 1,970,065 + BBR_RS18645 0.25 +0.9 1,970,084 - BBR_RS18645 0.27 +0.7 1,970,094 + BBR_RS18645 0.29 +1.5 1,970,120 - BBR_RS18645 0.33 +0.5 1,970,120 - BBR_RS18645 0.33 +0.2 1,970,145 + BBR_RS18645 0.36 +0.2 1,970,145 + BBR_RS18645 0.36 +0.7 1,970,148 + BBR_RS18645 0.37 +0.4 1,970,148 - BBR_RS18645 0.37 +0.6 1,970,158 - BBR_RS18645 0.38 +0.1 1,970,158 - BBR_RS18645 0.38 +1.4 1,970,166 + BBR_RS18645 0.39 +0.5 1,970,174 - BBR_RS18645 0.41 +1.0 1,970,186 - BBR_RS18645 0.42 +0.8 1,970,191 - BBR_RS18645 0.43 +0.7 1,970,211 + BBR_RS18645 0.46 +0.5 1,970,211 + BBR_RS18645 0.46 -0.5 1,970,211 + BBR_RS18645 0.46 +0.2 1,970,211 + BBR_RS18645 0.46 +0.4 1,970,211 + BBR_RS18645 0.46 +0.0 1,970,211 + BBR_RS18645 0.46 +0.8 1,970,211 + BBR_RS18645 0.46 +0.5 1,970,219 - BBR_RS18645 0.47 +0.2 1,970,282 - BBR_RS18645 0.56 +1.2 1,970,282 - BBR_RS18645 0.56 +0.6 1,970,296 + BBR_RS18645 0.58 +1.0 1,970,296 + BBR_RS18645 0.58 +0.4 1,970,390 - BBR_RS18645 0.72 -0.2 1,970,443 + BBR_RS18645 0.80 +1.4 1,970,443 + BBR_RS18645 0.80 -0.0 1,970,451 - BBR_RS18645 0.81 +0.6 1,970,451 - BBR_RS18645 0.81 +0.4 1,970,487 + BBR_RS18645 0.86 +0.2 1,970,495 - BBR_RS18645 0.88 +0.5 1,970,511 - +0.4 1,970,520 - +0.2 1,970,542 + -0.2 1,970,557 + +0.3 1,970,557 + +0.6 1,970,581 + +0.5 1,970,589 - +0.2 1,970,608 - -0.5 1,970,632 + +0.3 1,970,632 + -0.6 1,970,640 - -0.1 1,970,663 + +0.3 1,970,724 + +0.2 1,970,732 - -0.2
Or see this region's nucleotide sequence