Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18615 and BBR_RS18620 are separated by 9 nucleotides BBR_RS18620 and BBR_RS18625 are separated by 17 nucleotides BBR_RS18625 and BBR_RS18630 are separated by 120 nucleotides BBR_RS18630 and BBR_RS18635 overlap by 4 nucleotides
BBR_RS18615: BBR_RS18615 - DUF4037 domain-containing protein, at 1,961,380 to 1,963,581
_RS18615
BBR_RS18620: BBR_RS18620 - DUF4125 domain-containing protein, at 1,963,591 to 1,964,223
_RS18620
BBR_RS18625: BBR_RS18625 - alpha/beta hydrolase, at 1,964,241 to 1,965,965
_RS18625
BBR_RS18630: BBR_RS18630 - hypothetical protein, at 1,966,086 to 1,966,805
_RS18630
BBR_RS18635: BBR_RS18635 - PspC domain-containing protein, at 1,966,802 to 1,968,484
_RS18635
Position (kb)
1964
1965
1966 Strain fitness (log2 ratio)
-1
0
1 at 1963.270 kb on - strand, within BBR_RS18615 at 1963.280 kb on - strand, within BBR_RS18615 at 1963.293 kb on + strand, within BBR_RS18615 at 1963.293 kb on + strand, within BBR_RS18615 at 1963.293 kb on + strand, within BBR_RS18615 at 1963.301 kb on - strand, within BBR_RS18615 at 1963.326 kb on + strand, within BBR_RS18615 at 1963.351 kb on + strand, within BBR_RS18615 at 1963.406 kb on + strand at 1963.474 kb on + strand at 1963.486 kb on + strand at 1963.489 kb on - strand at 1963.494 kb on - strand at 1963.519 kb on - strand at 1963.520 kb on + strand at 1963.535 kb on - strand at 1963.535 kb on - strand at 1963.545 kb on + strand at 1963.557 kb on - strand at 1963.559 kb on + strand at 1963.566 kb on + strand at 1963.566 kb on + strand at 1963.566 kb on + strand at 1963.574 kb on - strand at 1963.595 kb on + strand at 1963.642 kb on + strand at 1963.642 kb on + strand at 1963.664 kb on + strand, within BBR_RS18620 at 1963.672 kb on - strand, within BBR_RS18620 at 1963.758 kb on + strand, within BBR_RS18620 at 1963.759 kb on - strand, within BBR_RS18620 at 1963.777 kb on + strand, within BBR_RS18620 at 1963.785 kb on - strand, within BBR_RS18620 at 1963.790 kb on - strand, within BBR_RS18620 at 1963.847 kb on - strand, within BBR_RS18620 at 1963.852 kb on + strand, within BBR_RS18620 at 1963.860 kb on - strand, within BBR_RS18620 at 1963.860 kb on - strand, within BBR_RS18620 at 1963.860 kb on - strand, within BBR_RS18620 at 1963.879 kb on - strand, within BBR_RS18620 at 1963.879 kb on - strand, within BBR_RS18620 at 1963.879 kb on - strand, within BBR_RS18620 at 1963.886 kb on + strand, within BBR_RS18620 at 1963.997 kb on - strand, within BBR_RS18620 at 1963.997 kb on - strand, within BBR_RS18620 at 1964.080 kb on - strand, within BBR_RS18620 at 1964.084 kb on + strand, within BBR_RS18620 at 1964.084 kb on + strand, within BBR_RS18620 at 1964.144 kb on - strand, within BBR_RS18620 at 1964.144 kb on - strand, within BBR_RS18620 at 1964.192 kb on + strand at 1964.192 kb on + strand at 1964.192 kb on - strand at 1964.200 kb on - strand at 1964.200 kb on - strand at 1964.200 kb on - strand at 1964.202 kb on + strand at 1964.202 kb on + strand at 1964.210 kb on - strand at 1964.220 kb on - strand at 1964.230 kb on + strand at 1964.236 kb on - strand at 1964.248 kb on - strand at 1964.250 kb on + strand at 1964.250 kb on + strand at 1964.250 kb on + strand at 1964.280 kb on - strand at 1964.282 kb on - strand at 1964.300 kb on + strand at 1964.329 kb on + strand at 1964.337 kb on - strand at 1964.349 kb on - strand at 1964.350 kb on + strand at 1964.358 kb on - strand at 1964.382 kb on - strand at 1964.451 kb on + strand, within BBR_RS18625 at 1964.451 kb on + strand, within BBR_RS18625 at 1964.468 kb on + strand, within BBR_RS18625 at 1964.476 kb on - strand, within BBR_RS18625 at 1964.480 kb on + strand, within BBR_RS18625 at 1964.496 kb on - strand, within BBR_RS18625 at 1964.496 kb on - strand, within BBR_RS18625 at 1964.531 kb on - strand, within BBR_RS18625 at 1964.541 kb on - strand, within BBR_RS18625 at 1964.541 kb on - strand, within BBR_RS18625 at 1964.543 kb on + strand, within BBR_RS18625 at 1964.543 kb on + strand, within BBR_RS18625 at 1964.551 kb on - strand, within BBR_RS18625 at 1964.551 kb on - strand, within BBR_RS18625 at 1964.556 kb on + strand, within BBR_RS18625 at 1964.559 kb on - strand, within BBR_RS18625 at 1964.559 kb on - strand, within BBR_RS18625 at 1964.564 kb on - strand, within BBR_RS18625 at 1964.565 kb on - strand, within BBR_RS18625 at 1964.565 kb on - strand, within BBR_RS18625 at 1964.571 kb on + strand, within BBR_RS18625 at 1964.575 kb on + strand, within BBR_RS18625 at 1964.581 kb on + strand, within BBR_RS18625 at 1964.589 kb on - strand, within BBR_RS18625 at 1964.614 kb on + strand, within BBR_RS18625 at 1964.614 kb on + strand, within BBR_RS18625 at 1964.622 kb on - strand, within BBR_RS18625 at 1964.623 kb on + strand, within BBR_RS18625 at 1964.662 kb on + strand, within BBR_RS18625 at 1964.662 kb on + strand, within BBR_RS18625 at 1964.700 kb on - strand, within BBR_RS18625 at 1964.701 kb on - strand, within BBR_RS18625 at 1964.740 kb on + strand, within BBR_RS18625 at 1964.751 kb on + strand, within BBR_RS18625 at 1964.759 kb on - strand, within BBR_RS18625 at 1964.787 kb on + strand, within BBR_RS18625 at 1964.787 kb on + strand, within BBR_RS18625 at 1964.787 kb on + strand, within BBR_RS18625 at 1964.795 kb on - strand, within BBR_RS18625 at 1964.809 kb on + strand, within BBR_RS18625 at 1964.852 kb on + strand, within BBR_RS18625 at 1964.871 kb on + strand, within BBR_RS18625 at 1964.907 kb on + strand, within BBR_RS18625 at 1964.917 kb on - strand, within BBR_RS18625 at 1964.924 kb on - strand, within BBR_RS18625 at 1964.934 kb on - strand, within BBR_RS18625 at 1964.964 kb on - strand, within BBR_RS18625 at 1964.964 kb on - strand, within BBR_RS18625 at 1964.977 kb on + strand, within BBR_RS18625 at 1964.988 kb on + strand, within BBR_RS18625 at 1964.996 kb on - strand, within BBR_RS18625 at 1965.002 kb on + strand, within BBR_RS18625 at 1965.002 kb on + strand, within BBR_RS18625 at 1965.003 kb on + strand, within BBR_RS18625 at 1965.004 kb on + strand, within BBR_RS18625 at 1965.021 kb on + strand, within BBR_RS18625 at 1965.036 kb on + strand, within BBR_RS18625 at 1965.041 kb on + strand, within BBR_RS18625 at 1965.041 kb on + strand, within BBR_RS18625 at 1965.041 kb on + strand, within BBR_RS18625 at 1965.078 kb on + strand, within BBR_RS18625 at 1965.086 kb on + strand, within BBR_RS18625 at 1965.086 kb on + strand, within BBR_RS18625 at 1965.090 kb on + strand, within BBR_RS18625 at 1965.093 kb on - strand, within BBR_RS18625 at 1965.116 kb on - strand, within BBR_RS18625 at 1965.158 kb on - strand, within BBR_RS18625 at 1965.162 kb on + strand, within BBR_RS18625 at 1965.162 kb on + strand, within BBR_RS18625 at 1965.164 kb on + strand, within BBR_RS18625 at 1965.170 kb on - strand, within BBR_RS18625 at 1965.196 kb on + strand, within BBR_RS18625 at 1965.232 kb on + strand, within BBR_RS18625 at 1965.243 kb on + strand, within BBR_RS18625 at 1965.261 kb on - strand, within BBR_RS18625 at 1965.283 kb on + strand, within BBR_RS18625 at 1965.290 kb on + strand, within BBR_RS18625 at 1965.290 kb on - strand, within BBR_RS18625 at 1965.290 kb on - strand, within BBR_RS18625 at 1965.290 kb on - strand, within BBR_RS18625 at 1965.317 kb on + strand, within BBR_RS18625 at 1965.363 kb on - strand, within BBR_RS18625 at 1965.363 kb on - strand, within BBR_RS18625 at 1965.391 kb on + strand, within BBR_RS18625 at 1965.443 kb on - strand, within BBR_RS18625 at 1965.484 kb on + strand, within BBR_RS18625 at 1965.492 kb on - strand, within BBR_RS18625 at 1965.497 kb on - strand, within BBR_RS18625 at 1965.543 kb on + strand, within BBR_RS18625 at 1965.551 kb on - strand, within BBR_RS18625 at 1965.551 kb on - strand, within BBR_RS18625 at 1965.551 kb on - strand, within BBR_RS18625 at 1965.553 kb on + strand, within BBR_RS18625 at 1965.561 kb on - strand, within BBR_RS18625 at 1965.575 kb on - strand, within BBR_RS18625 at 1965.575 kb on - strand, within BBR_RS18625 at 1965.582 kb on - strand, within BBR_RS18625 at 1965.590 kb on + strand, within BBR_RS18625 at 1965.593 kb on - strand, within BBR_RS18625 at 1965.593 kb on - strand, within BBR_RS18625 at 1965.612 kb on - strand, within BBR_RS18625 at 1965.613 kb on - strand, within BBR_RS18625 at 1965.690 kb on - strand, within BBR_RS18625 at 1965.722 kb on + strand, within BBR_RS18625 at 1965.753 kb on - strand, within BBR_RS18625 at 1965.753 kb on - strand, within BBR_RS18625 at 1965.841 kb on - strand at 1965.847 kb on + strand at 1965.847 kb on + strand at 1965.855 kb on - strand at 1965.859 kb on + strand at 1965.867 kb on - strand at 1965.891 kb on - strand at 1965.902 kb on - strand at 1965.946 kb on - strand at 1965.981 kb on - strand at 1965.986 kb on + strand at 1965.986 kb on + strand at 1965.987 kb on + strand at 1965.988 kb on + strand at 1965.994 kb on - strand at 1966.017 kb on + strand at 1966.025 kb on - strand at 1966.121 kb on - strand at 1966.835 kb on - strand at 1966.910 kb on - strand at 1966.917 kb on - strand at 1966.936 kb on - strand at 1966.953 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,963,270 - BBR_RS18615 0.86 -0.1 1,963,280 - BBR_RS18615 0.86 -0.2 1,963,293 + BBR_RS18615 0.87 +0.2 1,963,293 + BBR_RS18615 0.87 -1.1 1,963,293 + BBR_RS18615 0.87 +0.9 1,963,301 - BBR_RS18615 0.87 -0.6 1,963,326 + BBR_RS18615 0.88 -1.0 1,963,351 + BBR_RS18615 0.90 +0.5 1,963,406 + +0.3 1,963,474 + +0.1 1,963,486 + +0.9 1,963,489 - +1.0 1,963,494 - +0.5 1,963,519 - +0.3 1,963,520 + +0.2 1,963,535 - -0.1 1,963,535 - -0.2 1,963,545 + +0.3 1,963,557 - +0.8 1,963,559 + +0.6 1,963,566 + -0.2 1,963,566 + +0.4 1,963,566 + -0.5 1,963,574 - -0.1 1,963,595 + -0.7 1,963,642 + +0.3 1,963,642 + -0.3 1,963,664 + BBR_RS18620 0.12 -0.5 1,963,672 - BBR_RS18620 0.13 -0.4 1,963,758 + BBR_RS18620 0.26 +1.0 1,963,759 - BBR_RS18620 0.27 +0.4 1,963,777 + BBR_RS18620 0.29 +0.0 1,963,785 - BBR_RS18620 0.31 +0.2 1,963,790 - BBR_RS18620 0.31 +0.3 1,963,847 - BBR_RS18620 0.40 -0.9 1,963,852 + BBR_RS18620 0.41 -1.6 1,963,860 - BBR_RS18620 0.42 -0.4 1,963,860 - BBR_RS18620 0.42 +0.1 1,963,860 - BBR_RS18620 0.42 -0.4 1,963,879 - BBR_RS18620 0.45 +0.2 1,963,879 - BBR_RS18620 0.45 +0.8 1,963,879 - BBR_RS18620 0.45 -0.1 1,963,886 + BBR_RS18620 0.47 -0.3 1,963,997 - BBR_RS18620 0.64 +1.3 1,963,997 - BBR_RS18620 0.64 +0.2 1,964,080 - BBR_RS18620 0.77 -0.4 1,964,084 + BBR_RS18620 0.78 -0.2 1,964,084 + BBR_RS18620 0.78 +0.7 1,964,144 - BBR_RS18620 0.87 -0.4 1,964,144 - BBR_RS18620 0.87 +0.7 1,964,192 + +0.5 1,964,192 + +0.1 1,964,192 - -0.2 1,964,200 - +0.3 1,964,200 - -0.2 1,964,200 - +0.1 1,964,202 + -0.2 1,964,202 + +0.3 1,964,210 - +0.8 1,964,220 - +0.5 1,964,230 + +0.6 1,964,236 - +0.1 1,964,248 - -0.6 1,964,250 + +0.0 1,964,250 + +0.3 1,964,250 + +0.1 1,964,280 - +0.0 1,964,282 - +0.3 1,964,300 + +1.2 1,964,329 + +0.0 1,964,337 - -0.3 1,964,349 - +0.7 1,964,350 + +0.3 1,964,358 - -0.1 1,964,382 - -0.2 1,964,451 + BBR_RS18625 0.12 +0.6 1,964,451 + BBR_RS18625 0.12 -0.3 1,964,468 + BBR_RS18625 0.13 -0.8 1,964,476 - BBR_RS18625 0.14 -0.2 1,964,480 + BBR_RS18625 0.14 +0.6 1,964,496 - BBR_RS18625 0.15 +0.4 1,964,496 - BBR_RS18625 0.15 -0.2 1,964,531 - BBR_RS18625 0.17 +0.1 1,964,541 - BBR_RS18625 0.17 -0.6 1,964,541 - BBR_RS18625 0.17 +1.0 1,964,543 + BBR_RS18625 0.18 +0.2 1,964,543 + BBR_RS18625 0.18 +0.7 1,964,551 - BBR_RS18625 0.18 +0.3 1,964,551 - BBR_RS18625 0.18 +0.4 1,964,556 + BBR_RS18625 0.18 -0.7 1,964,559 - BBR_RS18625 0.18 +1.2 1,964,559 - BBR_RS18625 0.18 +0.6 1,964,564 - BBR_RS18625 0.19 -0.2 1,964,565 - BBR_RS18625 0.19 +0.5 1,964,565 - BBR_RS18625 0.19 -0.5 1,964,571 + BBR_RS18625 0.19 -0.1 1,964,575 + BBR_RS18625 0.19 +0.8 1,964,581 + BBR_RS18625 0.20 -0.2 1,964,589 - BBR_RS18625 0.20 -0.6 1,964,614 + BBR_RS18625 0.22 +0.5 1,964,614 + BBR_RS18625 0.22 -0.4 1,964,622 - BBR_RS18625 0.22 +0.2 1,964,623 + BBR_RS18625 0.22 +0.2 1,964,662 + BBR_RS18625 0.24 +0.5 1,964,662 + BBR_RS18625 0.24 -0.1 1,964,700 - BBR_RS18625 0.27 +0.4 1,964,701 - BBR_RS18625 0.27 +0.2 1,964,740 + BBR_RS18625 0.29 -0.6 1,964,751 + BBR_RS18625 0.30 +1.0 1,964,759 - BBR_RS18625 0.30 -0.0 1,964,787 + BBR_RS18625 0.32 -0.7 1,964,787 + BBR_RS18625 0.32 +0.6 1,964,787 + BBR_RS18625 0.32 -0.1 1,964,795 - BBR_RS18625 0.32 +0.1 1,964,809 + BBR_RS18625 0.33 -0.3 1,964,852 + BBR_RS18625 0.35 +0.4 1,964,871 + BBR_RS18625 0.37 +0.6 1,964,907 + BBR_RS18625 0.39 -0.4 1,964,917 - BBR_RS18625 0.39 -0.0 1,964,924 - BBR_RS18625 0.40 +0.9 1,964,934 - BBR_RS18625 0.40 +0.6 1,964,964 - BBR_RS18625 0.42 +0.2 1,964,964 - BBR_RS18625 0.42 +0.1 1,964,977 + BBR_RS18625 0.43 +0.5 1,964,988 + BBR_RS18625 0.43 +0.3 1,964,996 - BBR_RS18625 0.44 +0.8 1,965,002 + BBR_RS18625 0.44 +0.6 1,965,002 + BBR_RS18625 0.44 +1.1 1,965,003 + BBR_RS18625 0.44 +1.0 1,965,004 + BBR_RS18625 0.44 -0.7 1,965,021 + BBR_RS18625 0.45 +0.2 1,965,036 + BBR_RS18625 0.46 +0.6 1,965,041 + BBR_RS18625 0.46 -0.1 1,965,041 + BBR_RS18625 0.46 +0.3 1,965,041 + BBR_RS18625 0.46 +0.3 1,965,078 + BBR_RS18625 0.49 -0.1 1,965,086 + BBR_RS18625 0.49 +0.1 1,965,086 + BBR_RS18625 0.49 +1.0 1,965,090 + BBR_RS18625 0.49 +0.0 1,965,093 - BBR_RS18625 0.49 +0.5 1,965,116 - BBR_RS18625 0.51 +0.2 1,965,158 - BBR_RS18625 0.53 +0.1 1,965,162 + BBR_RS18625 0.53 +0.9 1,965,162 + BBR_RS18625 0.53 +1.0 1,965,164 + BBR_RS18625 0.54 +0.4 1,965,170 - BBR_RS18625 0.54 -0.4 1,965,196 + BBR_RS18625 0.55 +0.4 1,965,232 + BBR_RS18625 0.57 -1.2 1,965,243 + BBR_RS18625 0.58 +0.5 1,965,261 - BBR_RS18625 0.59 -0.2 1,965,283 + BBR_RS18625 0.60 +0.8 1,965,290 + BBR_RS18625 0.61 +0.3 1,965,290 - BBR_RS18625 0.61 -0.4 1,965,290 - BBR_RS18625 0.61 +0.4 1,965,290 - BBR_RS18625 0.61 +0.2 1,965,317 + BBR_RS18625 0.62 -0.5 1,965,363 - BBR_RS18625 0.65 +0.3 1,965,363 - BBR_RS18625 0.65 +0.2 1,965,391 + BBR_RS18625 0.67 +0.6 1,965,443 - BBR_RS18625 0.70 -0.1 1,965,484 + BBR_RS18625 0.72 +1.3 1,965,492 - BBR_RS18625 0.73 +0.6 1,965,497 - BBR_RS18625 0.73 +0.5 1,965,543 + BBR_RS18625 0.75 -0.1 1,965,551 - BBR_RS18625 0.76 +0.3 1,965,551 - BBR_RS18625 0.76 +0.3 1,965,551 - BBR_RS18625 0.76 -0.2 1,965,553 + BBR_RS18625 0.76 +0.2 1,965,561 - BBR_RS18625 0.77 +0.5 1,965,575 - BBR_RS18625 0.77 +0.9 1,965,575 - BBR_RS18625 0.77 +0.0 1,965,582 - BBR_RS18625 0.78 +0.7 1,965,590 + BBR_RS18625 0.78 -0.2 1,965,593 - BBR_RS18625 0.78 +0.1 1,965,593 - BBR_RS18625 0.78 +0.4 1,965,612 - BBR_RS18625 0.79 -0.8 1,965,613 - BBR_RS18625 0.80 +0.0 1,965,690 - BBR_RS18625 0.84 +0.3 1,965,722 + BBR_RS18625 0.86 -0.1 1,965,753 - BBR_RS18625 0.88 -0.4 1,965,753 - BBR_RS18625 0.88 +0.9 1,965,841 - +1.1 1,965,847 + +0.5 1,965,847 + +0.8 1,965,855 - +0.9 1,965,859 + -0.1 1,965,867 - -0.0 1,965,891 - +0.8 1,965,902 - -0.0 1,965,946 - +0.3 1,965,981 - +0.4 1,965,986 + +0.7 1,965,986 + -0.4 1,965,987 + +0.0 1,965,988 + -0.4 1,965,994 - +0.8 1,966,017 + +0.2 1,966,025 - -0.6 1,966,121 - -1.0 1,966,835 - -1.6 1,966,910 - -0.6 1,966,917 - +0.4 1,966,936 - -1.2 1,966,953 - -1.7
Or see this region's nucleotide sequence