Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18465 and BBR_RS21045 are separated by 70 nucleotides BBR_RS21045 and BBR_RS18470 are separated by 31 nucleotides BBR_RS18470 and BBR_RS18475 are separated by 367 nucleotides
BBR_RS18465: BBR_RS18465 - integrase, at 1,927,804 to 1,928,067
_RS18465
BBR_RS21045: BBR_RS21045 - hypothetical protein, at 1,928,138 to 1,928,536
_RS21045
BBR_RS18470: BBR_RS18470 - MFS transporter, at 1,928,568 to 1,930,049
_RS18470
BBR_RS18475: BBR_RS18475 - DUF4981 domain-containing protein, at 1,930,417 to 1,933,554
_RS18475
Position (kb) 1928
1929
1930
1931 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1927.590 kb on + strand at 1927.593 kb on - strand at 1927.593 kb on - strand at 1927.595 kb on + strand at 1927.595 kb on + strand at 1927.595 kb on + strand at 1927.595 kb on + strand at 1927.595 kb on + strand at 1927.595 kb on + strand at 1927.595 kb on + strand at 1927.600 kb on + strand at 1927.603 kb on - strand at 1927.603 kb on - strand at 1927.607 kb on + strand at 1927.608 kb on - strand at 1927.623 kb on + strand at 1927.623 kb on + strand at 1927.646 kb on - strand at 1927.646 kb on - strand at 1927.697 kb on + strand at 1927.697 kb on + strand at 1927.705 kb on + strand at 1927.705 kb on - strand at 1927.710 kb on + strand at 1927.813 kb on + strand at 1927.828 kb on + strand at 1927.828 kb on + strand at 1927.847 kb on - strand, within BBR_RS18465 at 1927.857 kb on - strand, within BBR_RS18465 at 1927.859 kb on + strand, within BBR_RS18465 at 1927.867 kb on - strand, within BBR_RS18465 at 1927.877 kb on - strand, within BBR_RS18465 at 1927.877 kb on - strand, within BBR_RS18465 at 1927.898 kb on + strand, within BBR_RS18465 at 1927.898 kb on + strand, within BBR_RS18465 at 1927.900 kb on + strand, within BBR_RS18465 at 1927.906 kb on + strand, within BBR_RS18465 at 1927.906 kb on + strand, within BBR_RS18465 at 1927.906 kb on - strand, within BBR_RS18465 at 1927.906 kb on - strand, within BBR_RS18465 at 1927.906 kb on - strand, within BBR_RS18465 at 1927.908 kb on - strand, within BBR_RS18465 at 1927.936 kb on - strand, within BBR_RS18465 at 1927.936 kb on - strand, within BBR_RS18465 at 1927.967 kb on + strand, within BBR_RS18465 at 1927.975 kb on + strand, within BBR_RS18465 at 1927.982 kb on - strand, within BBR_RS18465 at 1927.982 kb on - strand, within BBR_RS18465 at 1927.982 kb on - strand, within BBR_RS18465 at 1927.983 kb on + strand, within BBR_RS18465 at 1927.989 kb on + strand, within BBR_RS18465 at 1927.991 kb on - strand, within BBR_RS18465 at 1928.003 kb on + strand, within BBR_RS18465 at 1928.009 kb on + strand, within BBR_RS18465 at 1928.010 kb on - strand, within BBR_RS18465 at 1928.017 kb on - strand, within BBR_RS18465 at 1928.017 kb on - strand, within BBR_RS18465 at 1928.031 kb on + strand, within BBR_RS18465 at 1928.107 kb on + strand at 1928.113 kb on - strand at 1928.115 kb on - strand at 1928.115 kb on - strand at 1928.115 kb on - strand at 1928.115 kb on - strand at 1928.115 kb on - strand at 1928.115 kb on - strand at 1928.115 kb on - strand at 1928.115 kb on - strand at 1928.115 kb on - strand at 1928.137 kb on + strand at 1928.145 kb on - strand at 1928.150 kb on - strand at 1928.179 kb on + strand, within BBR_RS21045 at 1928.182 kb on - strand, within BBR_RS21045 at 1928.182 kb on - strand, within BBR_RS21045 at 1928.223 kb on - strand, within BBR_RS21045 at 1928.229 kb on - strand, within BBR_RS21045 at 1928.247 kb on + strand, within BBR_RS21045 at 1928.311 kb on - strand, within BBR_RS21045 at 1928.311 kb on - strand, within BBR_RS21045 at 1928.319 kb on - strand, within BBR_RS21045 at 1928.326 kb on + strand, within BBR_RS21045 at 1928.405 kb on + strand, within BBR_RS21045 at 1928.408 kb on - strand, within BBR_RS21045 at 1928.502 kb on + strand at 1928.537 kb on + strand at 1928.557 kb on + strand at 1928.565 kb on - strand at 1928.574 kb on - strand at 1928.702 kb on + strand at 1928.704 kb on - strand at 1928.721 kb on - strand, within BBR_RS18470 at 1928.722 kb on + strand, within BBR_RS18470 at 1928.803 kb on + strand, within BBR_RS18470 at 1928.803 kb on + strand, within BBR_RS18470 at 1928.803 kb on + strand, within BBR_RS18470 at 1928.821 kb on - strand, within BBR_RS18470 at 1928.841 kb on - strand, within BBR_RS18470 at 1928.848 kb on + strand, within BBR_RS18470 at 1928.860 kb on - strand, within BBR_RS18470 at 1928.886 kb on - strand, within BBR_RS18470 at 1928.936 kb on - strand, within BBR_RS18470 at 1928.938 kb on - strand, within BBR_RS18470 at 1928.960 kb on - strand, within BBR_RS18470 at 1929.009 kb on - strand, within BBR_RS18470 at 1929.073 kb on + strand, within BBR_RS18470 at 1929.085 kb on + strand, within BBR_RS18470 at 1929.085 kb on + strand, within BBR_RS18470 at 1929.085 kb on + strand, within BBR_RS18470 at 1929.093 kb on - strand, within BBR_RS18470 at 1929.093 kb on - strand, within BBR_RS18470 at 1929.105 kb on - strand, within BBR_RS18470 at 1929.105 kb on - strand, within BBR_RS18470 at 1929.115 kb on - strand, within BBR_RS18470 at 1929.214 kb on + strand, within BBR_RS18470 at 1929.219 kb on - strand, within BBR_RS18470 at 1929.233 kb on + strand, within BBR_RS18470 at 1929.246 kb on - strand, within BBR_RS18470 at 1929.246 kb on - strand, within BBR_RS18470 at 1929.268 kb on + strand, within BBR_RS18470 at 1929.288 kb on - strand, within BBR_RS18470 at 1929.292 kb on + strand, within BBR_RS18470 at 1929.300 kb on - strand, within BBR_RS18470 at 1929.414 kb on + strand, within BBR_RS18470 at 1929.529 kb on - strand, within BBR_RS18470 at 1929.531 kb on - strand, within BBR_RS18470 at 1929.603 kb on - strand, within BBR_RS18470 at 1929.612 kb on - strand, within BBR_RS18470 at 1929.677 kb on + strand, within BBR_RS18470 at 1929.689 kb on - strand, within BBR_RS18470 at 1929.689 kb on - strand, within BBR_RS18470 at 1929.693 kb on + strand, within BBR_RS18470 at 1929.736 kb on + strand, within BBR_RS18470 at 1929.736 kb on + strand, within BBR_RS18470 at 1929.737 kb on - strand, within BBR_RS18470 at 1929.804 kb on - strand, within BBR_RS18470 at 1929.828 kb on - strand, within BBR_RS18470 at 1929.865 kb on + strand, within BBR_RS18470 at 1929.873 kb on - strand, within BBR_RS18470 at 1929.877 kb on + strand, within BBR_RS18470 at 1929.890 kb on - strand, within BBR_RS18470 at 1930.013 kb on - strand at 1930.018 kb on - strand at 1930.029 kb on - strand at 1930.099 kb on + strand at 1930.122 kb on + strand at 1930.128 kb on - strand at 1930.128 kb on - strand at 1930.128 kb on - strand at 1930.128 kb on - strand at 1930.128 kb on - strand at 1930.128 kb on - strand at 1930.197 kb on + strand at 1930.212 kb on + strand at 1930.219 kb on - strand at 1930.230 kb on + strand at 1930.266 kb on + strand at 1930.274 kb on - strand at 1930.388 kb on + strand at 1930.408 kb on + strand at 1930.408 kb on + strand at 1930.424 kb on + strand at 1930.482 kb on + strand at 1930.515 kb on + strand at 1930.515 kb on + strand at 1930.539 kb on - strand at 1930.576 kb on + strand at 1930.588 kb on + strand at 1930.604 kb on - strand at 1930.727 kb on + strand at 1930.732 kb on + strand, within BBR_RS18475 at 1930.732 kb on + strand, within BBR_RS18475 at 1930.735 kb on - strand, within BBR_RS18475 at 1930.740 kb on - strand, within BBR_RS18475 at 1930.740 kb on - strand, within BBR_RS18475 at 1930.740 kb on - strand, within BBR_RS18475 at 1930.740 kb on - strand, within BBR_RS18475 at 1930.742 kb on + strand, within BBR_RS18475 at 1930.742 kb on - strand, within BBR_RS18475 at 1930.750 kb on - strand, within BBR_RS18475 at 1930.750 kb on - strand, within BBR_RS18475 at 1930.774 kb on + strand, within BBR_RS18475 at 1930.782 kb on - strand, within BBR_RS18475 at 1930.803 kb on + strand, within BBR_RS18475 at 1930.809 kb on + strand, within BBR_RS18475 at 1930.810 kb on + strand, within BBR_RS18475 at 1930.817 kb on - strand, within BBR_RS18475 at 1930.878 kb on + strand, within BBR_RS18475 at 1930.878 kb on + strand, within BBR_RS18475 at 1930.878 kb on + strand, within BBR_RS18475 at 1930.880 kb on + strand, within BBR_RS18475 at 1930.924 kb on + strand, within BBR_RS18475 at 1930.944 kb on + strand, within BBR_RS18475 at 1930.950 kb on + strand, within BBR_RS18475 at 1930.950 kb on + strand, within BBR_RS18475 at 1930.983 kb on + strand, within BBR_RS18475 at 1930.985 kb on + strand, within BBR_RS18475 at 1930.985 kb on + strand, within BBR_RS18475 at 1930.985 kb on + strand, within BBR_RS18475 at 1930.985 kb on + strand, within BBR_RS18475 at 1930.985 kb on + strand, within BBR_RS18475 at 1930.993 kb on - strand, within BBR_RS18475 at 1931.026 kb on + strand, within BBR_RS18475
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,927,590 + -0.4 1,927,593 - +0.3 1,927,593 - +0.1 1,927,595 + -0.2 1,927,595 + -0.7 1,927,595 + -0.3 1,927,595 + -0.3 1,927,595 + +0.5 1,927,595 + -1.1 1,927,595 + +1.0 1,927,600 + -0.6 1,927,603 - +0.5 1,927,603 - +0.2 1,927,607 + -0.4 1,927,608 - +1.5 1,927,623 + -1.6 1,927,623 + -0.4 1,927,646 - -1.2 1,927,646 - -1.2 1,927,697 + -0.0 1,927,697 + -0.1 1,927,705 + -1.5 1,927,705 - +1.0 1,927,710 + -0.6 1,927,813 + -0.1 1,927,828 + -0.3 1,927,828 + +0.2 1,927,847 - BBR_RS18465 0.16 +0.0 1,927,857 - BBR_RS18465 0.20 +0.2 1,927,859 + BBR_RS18465 0.21 -0.1 1,927,867 - BBR_RS18465 0.24 +0.7 1,927,877 - BBR_RS18465 0.28 -0.5 1,927,877 - BBR_RS18465 0.28 -0.7 1,927,898 + BBR_RS18465 0.36 +0.2 1,927,898 + BBR_RS18465 0.36 +0.4 1,927,900 + BBR_RS18465 0.36 -0.3 1,927,906 + BBR_RS18465 0.39 +0.8 1,927,906 + BBR_RS18465 0.39 +0.5 1,927,906 - BBR_RS18465 0.39 +1.1 1,927,906 - BBR_RS18465 0.39 +0.9 1,927,906 - BBR_RS18465 0.39 -0.1 1,927,908 - BBR_RS18465 0.39 +0.1 1,927,936 - BBR_RS18465 0.50 -1.2 1,927,936 - BBR_RS18465 0.50 -0.6 1,927,967 + BBR_RS18465 0.62 -0.3 1,927,975 + BBR_RS18465 0.65 +0.1 1,927,982 - BBR_RS18465 0.67 -0.7 1,927,982 - BBR_RS18465 0.67 +0.3 1,927,982 - BBR_RS18465 0.67 -0.9 1,927,983 + BBR_RS18465 0.68 +0.6 1,927,989 + BBR_RS18465 0.70 +0.7 1,927,991 - BBR_RS18465 0.71 +0.9 1,928,003 + BBR_RS18465 0.75 -0.3 1,928,009 + BBR_RS18465 0.78 +0.3 1,928,010 - BBR_RS18465 0.78 +0.5 1,928,017 - BBR_RS18465 0.81 +0.3 1,928,017 - BBR_RS18465 0.81 -0.1 1,928,031 + BBR_RS18465 0.86 -0.2 1,928,107 + +2.4 1,928,113 - +0.0 1,928,115 - -0.2 1,928,115 - +0.6 1,928,115 - -0.6 1,928,115 - +0.7 1,928,115 - -0.6 1,928,115 - +1.4 1,928,115 - +0.1 1,928,115 - +0.8 1,928,115 - +0.2 1,928,137 + +0.5 1,928,145 - -0.1 1,928,150 - +0.3 1,928,179 + BBR_RS21045 0.10 +0.8 1,928,182 - BBR_RS21045 0.11 -0.0 1,928,182 - BBR_RS21045 0.11 +0.7 1,928,223 - BBR_RS21045 0.21 +0.2 1,928,229 - BBR_RS21045 0.23 +0.2 1,928,247 + BBR_RS21045 0.27 +0.0 1,928,311 - BBR_RS21045 0.43 +0.3 1,928,311 - BBR_RS21045 0.43 -0.1 1,928,319 - BBR_RS21045 0.45 +0.6 1,928,326 + BBR_RS21045 0.47 -0.2 1,928,405 + BBR_RS21045 0.67 +0.1 1,928,408 - BBR_RS21045 0.68 -0.2 1,928,502 + +0.3 1,928,537 + -0.1 1,928,557 + +0.3 1,928,565 - -0.5 1,928,574 - -0.5 1,928,702 + +0.9 1,928,704 - +0.5 1,928,721 - BBR_RS18470 0.10 +0.9 1,928,722 + BBR_RS18470 0.10 +0.3 1,928,803 + BBR_RS18470 0.16 +0.0 1,928,803 + BBR_RS18470 0.16 -0.1 1,928,803 + BBR_RS18470 0.16 -0.3 1,928,821 - BBR_RS18470 0.17 +0.2 1,928,841 - BBR_RS18470 0.18 +0.9 1,928,848 + BBR_RS18470 0.19 +0.7 1,928,860 - BBR_RS18470 0.20 -0.2 1,928,886 - BBR_RS18470 0.21 +0.5 1,928,936 - BBR_RS18470 0.25 +0.1 1,928,938 - BBR_RS18470 0.25 +0.3 1,928,960 - BBR_RS18470 0.26 +0.5 1,929,009 - BBR_RS18470 0.30 -0.2 1,929,073 + BBR_RS18470 0.34 +0.5 1,929,085 + BBR_RS18470 0.35 +0.3 1,929,085 + BBR_RS18470 0.35 +0.1 1,929,085 + BBR_RS18470 0.35 +0.4 1,929,093 - BBR_RS18470 0.35 +0.5 1,929,093 - BBR_RS18470 0.35 +0.7 1,929,105 - BBR_RS18470 0.36 -0.0 1,929,105 - BBR_RS18470 0.36 -0.3 1,929,115 - BBR_RS18470 0.37 +0.4 1,929,214 + BBR_RS18470 0.44 -0.4 1,929,219 - BBR_RS18470 0.44 +0.5 1,929,233 + BBR_RS18470 0.45 -0.3 1,929,246 - BBR_RS18470 0.46 +0.4 1,929,246 - BBR_RS18470 0.46 +0.5 1,929,268 + BBR_RS18470 0.47 +1.1 1,929,288 - BBR_RS18470 0.49 +0.5 1,929,292 + BBR_RS18470 0.49 -0.9 1,929,300 - BBR_RS18470 0.49 -1.9 1,929,414 + BBR_RS18470 0.57 -0.2 1,929,529 - BBR_RS18470 0.65 +0.4 1,929,531 - BBR_RS18470 0.65 +0.4 1,929,603 - BBR_RS18470 0.70 +0.4 1,929,612 - BBR_RS18470 0.70 -0.7 1,929,677 + BBR_RS18470 0.75 +0.3 1,929,689 - BBR_RS18470 0.76 -0.4 1,929,689 - BBR_RS18470 0.76 +0.2 1,929,693 + BBR_RS18470 0.76 +0.4 1,929,736 + BBR_RS18470 0.79 -0.8 1,929,736 + BBR_RS18470 0.79 +0.4 1,929,737 - BBR_RS18470 0.79 +0.2 1,929,804 - BBR_RS18470 0.83 -0.1 1,929,828 - BBR_RS18470 0.85 -0.3 1,929,865 + BBR_RS18470 0.88 +0.9 1,929,873 - BBR_RS18470 0.88 -0.8 1,929,877 + BBR_RS18470 0.88 +0.3 1,929,890 - BBR_RS18470 0.89 +0.3 1,930,013 - +0.4 1,930,018 - +0.6 1,930,029 - +0.8 1,930,099 + +0.1 1,930,122 + +0.2 1,930,128 - +0.2 1,930,128 - -0.0 1,930,128 - -0.0 1,930,128 - -2.0 1,930,128 - +0.8 1,930,128 - -0.5 1,930,197 + +0.2 1,930,212 + +0.0 1,930,219 - -0.5 1,930,230 + +0.3 1,930,266 + +0.0 1,930,274 - -0.2 1,930,388 + +0.5 1,930,408 + +0.2 1,930,408 + -0.7 1,930,424 + +0.6 1,930,482 + +0.4 1,930,515 + -0.7 1,930,515 + +0.4 1,930,539 - -0.9 1,930,576 + +1.1 1,930,588 + +0.4 1,930,604 - -0.4 1,930,727 + -1.1 1,930,732 + BBR_RS18475 0.10 -0.2 1,930,732 + BBR_RS18475 0.10 +0.1 1,930,735 - BBR_RS18475 0.10 +0.1 1,930,740 - BBR_RS18475 0.10 +0.6 1,930,740 - BBR_RS18475 0.10 +0.4 1,930,740 - BBR_RS18475 0.10 -0.3 1,930,740 - BBR_RS18475 0.10 +0.4 1,930,742 + BBR_RS18475 0.10 -0.5 1,930,742 - BBR_RS18475 0.10 +0.6 1,930,750 - BBR_RS18475 0.11 -0.4 1,930,750 - BBR_RS18475 0.11 +0.3 1,930,774 + BBR_RS18475 0.11 +0.1 1,930,782 - BBR_RS18475 0.12 -0.0 1,930,803 + BBR_RS18475 0.12 -0.6 1,930,809 + BBR_RS18475 0.12 -0.3 1,930,810 + BBR_RS18475 0.13 +0.2 1,930,817 - BBR_RS18475 0.13 -0.0 1,930,878 + BBR_RS18475 0.15 +0.5 1,930,878 + BBR_RS18475 0.15 +0.2 1,930,878 + BBR_RS18475 0.15 +0.5 1,930,880 + BBR_RS18475 0.15 -0.4 1,930,924 + BBR_RS18475 0.16 +0.5 1,930,944 + BBR_RS18475 0.17 -0.0 1,930,950 + BBR_RS18475 0.17 +0.2 1,930,950 + BBR_RS18475 0.17 +0.4 1,930,983 + BBR_RS18475 0.18 -0.1 1,930,985 + BBR_RS18475 0.18 -0.6 1,930,985 + BBR_RS18475 0.18 -0.1 1,930,985 + BBR_RS18475 0.18 +0.4 1,930,985 + BBR_RS18475 0.18 -0.5 1,930,985 + BBR_RS18475 0.18 -1.2 1,930,993 - BBR_RS18475 0.18 +2.1 1,931,026 + BBR_RS18475 0.19 +0.3
Or see this region's nucleotide sequence