Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17870 and BBR_RS21025 overlap by 4 nucleotides BBR_RS21025 and BBR_RS17875 are separated by 56 nucleotides BBR_RS17875 and BBR_RS17880 are separated by 14 nucleotides BBR_RS17880 and BBR_RS17885 overlap by 14 nucleotides BBR_RS17885 and BBR_RS21030 are separated by 47 nucleotides
BBR_RS17870: BBR_RS17870 - glycosyl hydrolase, at 1,789,860 to 1,791,059
_RS17870
BBR_RS21025: BBR_RS21025 - hypothetical protein, at 1,791,056 to 1,791,396
_RS21025
BBR_RS17875: BBR_RS17875 - glucan 1,3-beta-glucosidase, at 1,791,453 to 1,792,658
_RS17875
BBR_RS17880: BBR_RS17880 - nucleotidyltransferase, at 1,792,673 to 1,793,074
_RS17880
BBR_RS17885: BBR_RS17885 - nucleotidyltransferase domain-containing protein, at 1,793,061 to 1,793,348
_RS17885
BBR_RS21030: BBR_RS21030 - hypothetical protein, at 1,793,396 to 1,793,761
_RS21030
Position (kb)
1791
1792
1793 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1790.461 kb on + strand, within BBR_RS17870 at 1790.466 kb on + strand, within BBR_RS17870 at 1790.471 kb on + strand, within BBR_RS17870 at 1790.471 kb on + strand, within BBR_RS17870 at 1790.479 kb on - strand, within BBR_RS17870 at 1790.479 kb on - strand, within BBR_RS17870 at 1790.486 kb on - strand, within BBR_RS17870 at 1790.498 kb on - strand, within BBR_RS17870 at 1790.509 kb on - strand, within BBR_RS17870 at 1790.509 kb on - strand, within BBR_RS17870 at 1790.510 kb on - strand, within BBR_RS17870 at 1790.575 kb on - strand, within BBR_RS17870 at 1790.594 kb on - strand, within BBR_RS17870 at 1790.638 kb on + strand, within BBR_RS17870 at 1790.638 kb on + strand, within BBR_RS17870 at 1790.646 kb on - strand, within BBR_RS17870 at 1790.646 kb on - strand, within BBR_RS17870 at 1790.677 kb on + strand, within BBR_RS17870 at 1790.685 kb on - strand, within BBR_RS17870 at 1790.721 kb on - strand, within BBR_RS17870 at 1790.743 kb on - strand, within BBR_RS17870 at 1790.747 kb on + strand, within BBR_RS17870 at 1790.755 kb on + strand, within BBR_RS17870 at 1790.763 kb on - strand, within BBR_RS17870 at 1790.763 kb on - strand, within BBR_RS17870 at 1790.763 kb on - strand, within BBR_RS17870 at 1790.763 kb on - strand, within BBR_RS17870 at 1790.776 kb on + strand, within BBR_RS17870 at 1790.808 kb on - strand, within BBR_RS17870 at 1790.810 kb on + strand, within BBR_RS17870 at 1790.814 kb on + strand, within BBR_RS17870 at 1790.827 kb on - strand, within BBR_RS17870 at 1790.892 kb on + strand, within BBR_RS17870 at 1790.892 kb on - strand, within BBR_RS17870 at 1790.899 kb on + strand, within BBR_RS17870 at 1790.954 kb on + strand at 1790.962 kb on - strand at 1790.962 kb on - strand at 1790.962 kb on - strand at 1790.962 kb on - strand at 1791.063 kb on + strand at 1791.068 kb on + strand at 1791.071 kb on - strand at 1791.089 kb on - strand at 1791.089 kb on - strand at 1791.096 kb on - strand, within BBR_RS21025 at 1791.179 kb on + strand, within BBR_RS21025 at 1791.187 kb on - strand, within BBR_RS21025 at 1791.195 kb on - strand, within BBR_RS21025 at 1791.197 kb on - strand, within BBR_RS21025 at 1791.200 kb on + strand, within BBR_RS21025 at 1791.223 kb on + strand, within BBR_RS21025 at 1791.243 kb on - strand, within BBR_RS21025 at 1791.246 kb on + strand, within BBR_RS21025 at 1791.272 kb on + strand, within BBR_RS21025 at 1791.278 kb on - strand, within BBR_RS21025 at 1791.301 kb on - strand, within BBR_RS21025 at 1791.301 kb on - strand, within BBR_RS21025 at 1791.301 kb on - strand, within BBR_RS21025 at 1791.370 kb on - strand at 1791.372 kb on + strand at 1791.372 kb on + strand at 1791.372 kb on + strand at 1791.372 kb on + strand at 1791.372 kb on + strand at 1791.372 kb on + strand at 1791.372 kb on + strand at 1791.372 kb on + strand at 1791.380 kb on - strand at 1791.380 kb on - strand at 1791.380 kb on - strand at 1791.380 kb on - strand at 1791.380 kb on - strand at 1791.380 kb on - strand at 1791.380 kb on - strand at 1791.380 kb on - strand at 1791.380 kb on - strand at 1791.380 kb on - strand at 1791.380 kb on - strand at 1791.396 kb on + strand at 1791.416 kb on + strand at 1791.439 kb on - strand at 1791.451 kb on + strand at 1791.472 kb on + strand at 1791.472 kb on + strand at 1791.472 kb on + strand at 1791.504 kb on - strand at 1791.505 kb on - strand at 1791.602 kb on - strand, within BBR_RS17875 at 1791.602 kb on - strand, within BBR_RS17875 at 1791.619 kb on - strand, within BBR_RS17875 at 1791.640 kb on - strand, within BBR_RS17875 at 1791.645 kb on + strand, within BBR_RS17875 at 1791.657 kb on + strand, within BBR_RS17875 at 1791.738 kb on - strand, within BBR_RS17875 at 1791.749 kb on - strand, within BBR_RS17875 at 1791.753 kb on - strand, within BBR_RS17875 at 1791.763 kb on - strand, within BBR_RS17875 at 1791.809 kb on + strand, within BBR_RS17875 at 1791.817 kb on - strand, within BBR_RS17875 at 1791.817 kb on - strand, within BBR_RS17875 at 1791.842 kb on - strand, within BBR_RS17875 at 1791.868 kb on + strand, within BBR_RS17875 at 1791.868 kb on + strand, within BBR_RS17875 at 1791.868 kb on + strand, within BBR_RS17875 at 1791.868 kb on + strand, within BBR_RS17875 at 1791.868 kb on + strand, within BBR_RS17875 at 1791.876 kb on - strand, within BBR_RS17875 at 1791.890 kb on - strand, within BBR_RS17875 at 1791.925 kb on + strand, within BBR_RS17875 at 1791.931 kb on - strand, within BBR_RS17875 at 1792.035 kb on - strand, within BBR_RS17875 at 1792.035 kb on - strand, within BBR_RS17875 at 1792.056 kb on + strand, within BBR_RS17875 at 1792.059 kb on - strand, within BBR_RS17875 at 1792.060 kb on - strand, within BBR_RS17875 at 1792.065 kb on - strand, within BBR_RS17875 at 1792.067 kb on - strand, within BBR_RS17875 at 1792.075 kb on - strand, within BBR_RS17875 at 1792.082 kb on - strand, within BBR_RS17875 at 1792.249 kb on + strand, within BBR_RS17875 at 1792.259 kb on - strand, within BBR_RS17875 at 1792.265 kb on + strand, within BBR_RS17875 at 1792.265 kb on + strand, within BBR_RS17875 at 1792.302 kb on - strand, within BBR_RS17875 at 1792.304 kb on - strand, within BBR_RS17875 at 1792.336 kb on - strand, within BBR_RS17875 at 1792.336 kb on - strand, within BBR_RS17875 at 1792.336 kb on - strand, within BBR_RS17875 at 1792.336 kb on - strand, within BBR_RS17875 at 1792.346 kb on - strand, within BBR_RS17875 at 1792.346 kb on - strand, within BBR_RS17875 at 1792.371 kb on - strand, within BBR_RS17875 at 1792.393 kb on - strand, within BBR_RS17875 at 1792.411 kb on + strand, within BBR_RS17875 at 1792.432 kb on - strand, within BBR_RS17875 at 1792.473 kb on - strand, within BBR_RS17875 at 1792.484 kb on - strand, within BBR_RS17875 at 1792.496 kb on - strand, within BBR_RS17875 at 1792.498 kb on + strand, within BBR_RS17875 at 1792.498 kb on + strand, within BBR_RS17875 at 1792.506 kb on - strand, within BBR_RS17875 at 1792.508 kb on - strand, within BBR_RS17875 at 1792.508 kb on - strand, within BBR_RS17875 at 1792.515 kb on + strand, within BBR_RS17875 at 1792.532 kb on + strand, within BBR_RS17875 at 1792.618 kb on + strand at 1792.626 kb on - strand at 1792.639 kb on - strand at 1792.641 kb on - strand at 1792.655 kb on - strand at 1792.663 kb on - strand at 1792.712 kb on - strand at 1792.759 kb on + strand, within BBR_RS17880 at 1792.780 kb on - strand, within BBR_RS17880 at 1792.783 kb on - strand, within BBR_RS17880 at 1792.791 kb on - strand, within BBR_RS17880 at 1792.879 kb on + strand, within BBR_RS17880 at 1792.923 kb on + strand, within BBR_RS17880 at 1792.932 kb on + strand, within BBR_RS17880 at 1793.434 kb on - strand, within BBR_RS21030 at 1793.445 kb on - strand, within BBR_RS21030 at 1793.560 kb on + strand, within BBR_RS21030 at 1793.560 kb on + strand, within BBR_RS21030 at 1793.563 kb on - strand, within BBR_RS21030 at 1793.568 kb on - strand, within BBR_RS21030 at 1793.591 kb on + strand, within BBR_RS21030 at 1793.591 kb on + strand, within BBR_RS21030 at 1793.613 kb on - strand, within BBR_RS21030 at 1793.613 kb on - strand, within BBR_RS21030
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,790,461 + BBR_RS17870 0.50 -1.2 1,790,466 + BBR_RS17870 0.51 -0.4 1,790,471 + BBR_RS17870 0.51 -0.1 1,790,471 + BBR_RS17870 0.51 +0.5 1,790,479 - BBR_RS17870 0.52 -0.4 1,790,479 - BBR_RS17870 0.52 +0.1 1,790,486 - BBR_RS17870 0.52 +0.5 1,790,498 - BBR_RS17870 0.53 +0.2 1,790,509 - BBR_RS17870 0.54 +0.4 1,790,509 - BBR_RS17870 0.54 -0.3 1,790,510 - BBR_RS17870 0.54 +0.3 1,790,575 - BBR_RS17870 0.60 +0.4 1,790,594 - BBR_RS17870 0.61 +0.5 1,790,638 + BBR_RS17870 0.65 +0.2 1,790,638 + BBR_RS17870 0.65 +0.2 1,790,646 - BBR_RS17870 0.66 -0.4 1,790,646 - BBR_RS17870 0.66 +0.2 1,790,677 + BBR_RS17870 0.68 -0.2 1,790,685 - BBR_RS17870 0.69 -0.1 1,790,721 - BBR_RS17870 0.72 -0.4 1,790,743 - BBR_RS17870 0.74 -0.5 1,790,747 + BBR_RS17870 0.74 -0.2 1,790,755 + BBR_RS17870 0.75 +1.7 1,790,763 - BBR_RS17870 0.75 -0.3 1,790,763 - BBR_RS17870 0.75 +0.5 1,790,763 - BBR_RS17870 0.75 +0.1 1,790,763 - BBR_RS17870 0.75 -0.4 1,790,776 + BBR_RS17870 0.76 +0.7 1,790,808 - BBR_RS17870 0.79 +1.0 1,790,810 + BBR_RS17870 0.79 -0.7 1,790,814 + BBR_RS17870 0.80 -0.4 1,790,827 - BBR_RS17870 0.81 -1.1 1,790,892 + BBR_RS17870 0.86 -1.7 1,790,892 - BBR_RS17870 0.86 -0.8 1,790,899 + BBR_RS17870 0.87 +0.2 1,790,954 + -0.5 1,790,962 - -1.9 1,790,962 - +0.0 1,790,962 - -1.2 1,790,962 - -0.4 1,791,063 + +0.8 1,791,068 + -0.0 1,791,071 - +0.1 1,791,089 - -0.5 1,791,089 - -0.9 1,791,096 - BBR_RS21025 0.12 -0.1 1,791,179 + BBR_RS21025 0.36 +0.1 1,791,187 - BBR_RS21025 0.38 -0.3 1,791,195 - BBR_RS21025 0.41 -0.3 1,791,197 - BBR_RS21025 0.41 -1.4 1,791,200 + BBR_RS21025 0.42 +0.1 1,791,223 + BBR_RS21025 0.49 -1.4 1,791,243 - BBR_RS21025 0.55 -0.1 1,791,246 + BBR_RS21025 0.56 -0.6 1,791,272 + BBR_RS21025 0.63 +1.2 1,791,278 - BBR_RS21025 0.65 -0.1 1,791,301 - BBR_RS21025 0.72 +0.3 1,791,301 - BBR_RS21025 0.72 +0.6 1,791,301 - BBR_RS21025 0.72 +0.1 1,791,370 - +0.2 1,791,372 + -0.8 1,791,372 + +0.1 1,791,372 + -0.3 1,791,372 + -0.2 1,791,372 + +0.1 1,791,372 + -0.7 1,791,372 + +1.1 1,791,372 + -0.5 1,791,380 - +0.1 1,791,380 - +0.3 1,791,380 - -0.5 1,791,380 - +0.9 1,791,380 - -0.1 1,791,380 - +0.4 1,791,380 - +0.6 1,791,380 - +0.3 1,791,380 - -1.6 1,791,380 - +0.1 1,791,380 - -1.7 1,791,396 + -0.6 1,791,416 + +0.5 1,791,439 - -0.2 1,791,451 + -0.7 1,791,472 + -0.5 1,791,472 + +1.3 1,791,472 + +0.3 1,791,504 - -0.2 1,791,505 - -0.1 1,791,602 - BBR_RS17875 0.12 +2.4 1,791,602 - BBR_RS17875 0.12 -1.0 1,791,619 - BBR_RS17875 0.14 -0.6 1,791,640 - BBR_RS17875 0.16 +0.7 1,791,645 + BBR_RS17875 0.16 +0.5 1,791,657 + BBR_RS17875 0.17 +0.0 1,791,738 - BBR_RS17875 0.24 +0.4 1,791,749 - BBR_RS17875 0.25 +0.3 1,791,753 - BBR_RS17875 0.25 +0.8 1,791,763 - BBR_RS17875 0.26 +1.1 1,791,809 + BBR_RS17875 0.30 -0.2 1,791,817 - BBR_RS17875 0.30 +0.1 1,791,817 - BBR_RS17875 0.30 +0.3 1,791,842 - BBR_RS17875 0.32 +0.4 1,791,868 + BBR_RS17875 0.34 +1.2 1,791,868 + BBR_RS17875 0.34 -1.3 1,791,868 + BBR_RS17875 0.34 -1.1 1,791,868 + BBR_RS17875 0.34 +0.1 1,791,868 + BBR_RS17875 0.34 +0.1 1,791,876 - BBR_RS17875 0.35 +0.4 1,791,890 - BBR_RS17875 0.36 +0.7 1,791,925 + BBR_RS17875 0.39 +0.6 1,791,931 - BBR_RS17875 0.40 -1.1 1,792,035 - BBR_RS17875 0.48 +0.6 1,792,035 - BBR_RS17875 0.48 -0.6 1,792,056 + BBR_RS17875 0.50 -0.2 1,792,059 - BBR_RS17875 0.50 +1.2 1,792,060 - BBR_RS17875 0.50 -1.4 1,792,065 - BBR_RS17875 0.51 +2.1 1,792,067 - BBR_RS17875 0.51 +0.0 1,792,075 - BBR_RS17875 0.52 +0.5 1,792,082 - BBR_RS17875 0.52 +0.5 1,792,249 + BBR_RS17875 0.66 +0.8 1,792,259 - BBR_RS17875 0.67 -0.1 1,792,265 + BBR_RS17875 0.67 +0.5 1,792,265 + BBR_RS17875 0.67 -0.8 1,792,302 - BBR_RS17875 0.70 -0.0 1,792,304 - BBR_RS17875 0.71 +1.0 1,792,336 - BBR_RS17875 0.73 +1.8 1,792,336 - BBR_RS17875 0.73 -0.5 1,792,336 - BBR_RS17875 0.73 +0.4 1,792,336 - BBR_RS17875 0.73 +0.6 1,792,346 - BBR_RS17875 0.74 +2.0 1,792,346 - BBR_RS17875 0.74 +0.6 1,792,371 - BBR_RS17875 0.76 +0.5 1,792,393 - BBR_RS17875 0.78 -0.1 1,792,411 + BBR_RS17875 0.79 +0.5 1,792,432 - BBR_RS17875 0.81 -1.1 1,792,473 - BBR_RS17875 0.85 -0.3 1,792,484 - BBR_RS17875 0.85 +1.2 1,792,496 - BBR_RS17875 0.86 +0.2 1,792,498 + BBR_RS17875 0.87 +0.5 1,792,498 + BBR_RS17875 0.87 +0.2 1,792,506 - BBR_RS17875 0.87 +0.2 1,792,508 - BBR_RS17875 0.87 -0.1 1,792,508 - BBR_RS17875 0.87 -0.9 1,792,515 + BBR_RS17875 0.88 +0.1 1,792,532 + BBR_RS17875 0.89 -0.2 1,792,618 + -0.1 1,792,626 - -1.4 1,792,639 - +0.4 1,792,641 - -0.5 1,792,655 - +0.1 1,792,663 - +0.2 1,792,712 - +2.0 1,792,759 + BBR_RS17880 0.21 +1.3 1,792,780 - BBR_RS17880 0.27 -1.3 1,792,783 - BBR_RS17880 0.27 +0.6 1,792,791 - BBR_RS17880 0.29 +0.7 1,792,879 + BBR_RS17880 0.51 -0.1 1,792,923 + BBR_RS17880 0.62 -1.1 1,792,932 + BBR_RS17880 0.64 -0.2 1,793,434 - BBR_RS21030 0.10 -0.5 1,793,445 - BBR_RS21030 0.13 -0.3 1,793,560 + BBR_RS21030 0.45 +0.1 1,793,560 + BBR_RS21030 0.45 -0.8 1,793,563 - BBR_RS21030 0.46 -0.6 1,793,568 - BBR_RS21030 0.47 -0.4 1,793,591 + BBR_RS21030 0.53 +0.7 1,793,591 + BBR_RS21030 0.53 -1.5 1,793,613 - BBR_RS21030 0.59 -0.7 1,793,613 - BBR_RS21030 0.59 -0.3
Or see this region's nucleotide sequence